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Citations for Mirus Bio :
51 - 82 of 82 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were seeded into 6-well plates and transfected the next day with 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). A3B-EGFP or A3A-EGFP expression was induced 6 hours after transfection with 50 ng/mL doxycycline ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus). Viruses from cell culture supernatants were passaged on M2-10B4 cells followed by virus purification and titration [3] ...
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bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
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bioRxiv - Molecular Biology 2024Quote: ... VERO and HEK293-Flp-In™-T-REx™ were produced by seeding 100,000 cells in a 24-well plate and transfecting 800 ng of the corresponding plasmids using TransIT®-LT1 (Mirus Bio). The medium was changed after 24 h incubation.
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bioRxiv - Immunology 2024Quote: HEK293T cells were seeded at 1–1.5 × 105 cells/well in 24-well plates the day before transfection using TransIT-LT1 reagent at 1.5 µL transfection reagent/well (Mirus Bio LLC). One hundred ng of indicated constructs encoding an N-terminal mCherry-tagged CARD8 were co-transfected into HEK293T cells with empty vector (‘–’) ...
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bioRxiv - Immunology 2024Quote: ... were seeded at 2.25 x 105 cell/well in 24-well plates the day before transfection using TransIT-LT1 reagent at 1.5uL transfection reagent/well (Mirus Bio LLC). Functional inflammasomes were reconstituted by transfecting in 5ng human CASP1 and 100ng human IL-1β ...
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bioRxiv - Microbiology 2021Quote: Transient transfection experiments were performed in six-well plates at 2.5 × 105 HEK 293 T cells per well using TransIT®-LT1 transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions unless indicated.
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bioRxiv - Cell Biology 2022Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7 μg of the vector ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: 5400), or siRNA treatment (50 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... MEF cells were grown in 6-well plates to 80% confluency before transfection with RP-sgRNA11204G (1.5ug/well) using TransIT®-mRNA Transfection Kit (Mirus Bio LLC). After 24h post transfection ...
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bioRxiv - Microbiology 2020Quote: ... Monolayers of BHK-T7 cells at ~50% confluency in 6-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.8 μg of each of ten plasmid constructs representing the T1L or T3DI reovirus genome ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of viral genomic RNA was transfected per well in 12-well plates through the TransIT®-mRNA Transfection Kit (Mirus Bio) following manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: 3×105cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Immunology 2024Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7Lμg of the vector ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 x 105 293T cells per well were seeded in 6-well plates and transfected one day later with GFP-Mpro-L plasmids using TransIT-pro (Mirus Bio LLC). After 10 hours ...
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bioRxiv - Microbiology 2023Quote: 3 x 105 cells were seeded per well in 6-well plates and transfected one day after seeding with 3CLpro plasmids using TransIT-PRO (Mirus Bio LLC) and incubated 8-9 hours for Mpro-On and 10 hours for Mpro-Off assays ...
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bioRxiv - Microbiology 2022Quote: 293T cells were seeded at 2 × 105 cells/well in 6-well plates the day before transfection using TransIT-LT1 reagent (Mirus Bio LLC) at 3 μL transfection reagent/well as previously described 57 ...
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bioRxiv - Microbiology 2022Quote: HEK293T were seeded at 2 × 105 cells/well in 6-well plates the day before transfection using TransIT-LT1 reagent (Mirus Bio LLC) at 5.8 μL transfection reagent/well ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were seeded in 6-well plates and transfected 24hrs later with 2ug of pBS-(CBR-TCR(PTC))-(CBG-TCR(WT)) plasmid68 using TransIT-LT1® (Mirus, MIR2300), following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: 293T cells were seeded at 2-3×105 cells/well in six-well plates the day before transfection using TransIT-LT1 reagent (Mirus Bio LLC) at 3 µL transfection reagent/well as previously described (OhAinle et al. ...
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bioRxiv - Microbiology 2022Quote: 3×105 cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Cell Biology 2022Quote: Monoclonal and polyclonal hIPSC-dCas9 lines were transfected with pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep or pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock vectors as replicates in 12 well plates using TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR2300) according to manufacturer’s instructions and as detailed above ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded in 10-cm dishes or 6-well plates and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... Vero E6 cells cultured in 24-well plates were transfected with a mixture of 25 nM siRNAs using the IT-X2 reagent (Mirus Bio LLC, USA). A second transfection was performed 24 h after the first transfection ...
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bioRxiv - Pathology 2021Quote: ... Ad293 cells were cultured in 96 well plates with DMEM containing 10% FB Essence (Avantor Seradigm, USA) and transfected using Transit-LT1 (Mirus Bio, Madison, WI, USA) with plasmid encoding 4 LXR response elements fused with luciferase ...
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bioRxiv - Microbiology 2023Quote: ... were grown in 6-well plates and transfected the next day with 2.5 μg DNA with TransIT-LT1 (Mirus Bio catalog number MIR-2304) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... was then transfected into SH-SY5Y cells (50-60% confluent) in a 6-well plate using TransIT LT-1 reagent (Mirus Bio, Cat# MIR-2300). After 48 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach 50%-70% confluency before transient transfection using TransIT-2020 (Mirus Bio, catalog #: MIR 5400) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... 293T cells were seeded in 24 well plates at 2×104 cells per plate and then transfected the next day with pYX014 or pYX017 (100 ng) using TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI53711, USA). All transfections were performed using equal number of tested plasmid DNA molecules ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10 cm dishes and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus Bio, Madison, WI, catalog #: MIR 5400) according to the manufacturer’s instruction ...