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Citations for Mirus Bio :
51 - 97 of 97 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Aag2 and U4.4 cells were transfected with 100 ng dsRNA and 250 ng RepDV2Rluc RNA per well using the TransIT-mRNA transfection kit (Mirus). As a positive control ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Microbiology 2022Quote: ... Control and KO cells were seeded at 40,000 per 48 well and transfected with 200 μg RNA in 25 μL OptiMEM using Trans-IT mRNA Transfection Kit (Mirus) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs are transfected with ETV2 mmRNA in mTeSR™-E8™ complete media using TransIT reagent and mRNA boost ((Mirus Bio) One hour prior to transfection ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 cell monolayers were mock-transfected or transfected with sHCoV genomic RNA (1 μg/ml) using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C ...
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bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
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bioRxiv - Molecular Biology 2022Quote: ... RNAs encoding green fluorescent protein (GFP) were complexed with TransIT (Mirus Bio, Madison, WI) according to the manufacturer’s instructions by diluting in EMEM without serum or antibiotics and incubating for 2-5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... MEF cells were grown in 6-well plates to 80% confluency before transfection with RP-sgRNA11204G (1.5ug/well) using TransIT®-mRNA Transfection Kit (Mirus Bio LLC). After 24h post transfection ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transfected with 1 μg of in vitro-transcribed J6/JFH-1 Rluc RNA or H77S.3/GLuc RNA using the TransIT mRNA transfection kit (Mirus Bio LLC) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... were used to transfect 1 × 106 Huh-7 cells using the TransIT® mRNA transfection kit according to the manufacturer’s instructions (Mirus Bio LLC). At 72 h post transfection (p.t.) ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of viral genomic RNA was transfected per well in 12-well plates through the TransIT®-mRNA Transfection Kit (Mirus Bio) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... or control XRNA (RNA segment derived from the hepatitis C virus genome adjacent to and equivalent in length to polyU/UC that cannot induce RIG-I-dependent innate immune signaling8) using the TransIT®-mRNA Transfection kit (Mirus Bio LLC). Cells were collected in RLT buffer (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... SEAP expressing plasmid pSEAP was generated by substitution of eGFP coding sequence in pEGFP-C1 with sequence coding for SEAP in pLIVE® In Vivo Expression/Reporter Vectors (Mirus MIR 5620). SEAP integration into pSEAP plasmid sequence was checked by sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... overnight and transiently co-transfected with 0.25 μg IFITM3-YFP and 0.25 μg mCherry or 0.25 μg IFITM3-YFP and 0.25 μg IFITM3-mCherry using TransIT-293 (Mirus). Constructs encoded WT IFITM3 or IFITM3 G95L and YFP/mCherry was fused to the amino-terminus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... overnight and transiently with 0.50 μg IFITM3-mCherry using TransIT-293 (Mirus). Living cells in Fluorobrite DMEM (Gibco ...
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bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
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bioRxiv - Cell Biology 2023Quote: ... pCMV R8.74 and pCDH1-CMV-ATG3-mCherry-SV40-Hygromycin using TransIT-LT1 reagent (Mirus). After 72 hr post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... plasmid vectors coding the glycoproteins were transfected into BSR-T7 cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR 2300). At 16 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with the chemicals for 30 min before a transfection mixture containing 0.2 μg mCherry-expressing plasmid and 0.6 μl TransIT293 (Mirus) was added to the cells ...
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bioRxiv - Developmental Biology 2023Quote: ... we transfected S2 cells with pAC5.1-mcd8-CIBN-NTEV-NICD-GFP and pAC5.1-Cry2-mCherry-CTEV using the TransIT-Insect Reagent (Mirus, MIR #6104) according to manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were grown on coverslips and transiently transfected with mCherry Parkin and PPP3CB-Flag using Trans-IT transfection reagent (Mirus Bio) for 18 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 250 ng of plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h cells were treated with 1 µM Pon A or DMSO as a control ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h the cells were treated with either DMSO or 1 µM ponasterone A (Pon A) ...
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1.5µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167µg of pMD2.G and 1.3µg of p8.91) with TransIT-293 (Mirus Bio). After 2-3 days of transfection ...
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bioRxiv - Microbiology 2021Quote: ... Transient transfections for protein expression in 293T were performed with Transit-LT1 transfection reagent (Mirus Biotech) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... individual wells were transfected with plasmids encoding Venus fused BH3-proteins using TransIT-X2 reagent (Mirus). Non-transfected wells were treated with transfection reagent alone (no DNA added) ...
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bioRxiv - Developmental Biology 2021Quote: ... protein expression (15 ng/well) and target (4.5 ng/well) plasmids using TransIT-2020 Transfection Reagent (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Constructs were mixed with 0.5μg helper (encoding gag/pol) and 0.1μg encoding coat protein (pMD2) along with 6μL TransIT-Lenti transfection reagent (MIR6603; Mirus Bio; Madison, WI) in 200uL Opti-MEM (31985062 ...
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bioRxiv - Systems Biology 2021Quote: ... and grown in complete media for 1 day before co-transfection of the mammalian expression vectors encoding GFP and viral protein of interest using TransIT-2020 transfection reagent (#MIR5404, Mirus). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in RPE1 or HeLa cells for 24–48 h using TransIT®-LT1 Transfection Reagent (Mirus Bio) or Neon Electroporation (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 250 ng of a pCDNA3.1(+) vector encoding for the SARS-CoV-2 spike protein using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Six hours post transfection (p.t.) ...
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bioRxiv - Cell Biology 2022Quote: ... cells in each well were transfected with a plasmid encoding GFP-2A-POI (protein of interest) using TransIT-2020 transfection reagent (Mirus #5404) as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...