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Citations for Bachem :
1 - 50 of 117 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Z-Arg-Leu-Arg-Gly-Gly-AMC (Bachem Bioscience), and its activity was continuously monitored at 360 nm (excitation ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 mM H-Gly-Pro-Arg-Pro-OH (GPRP, Bachem) added to the HEPES platelet dilution buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 1/50 (v/v) in the presence of 10 mM of Gly-Pro-Arg-Pro-NH2 peptide (Bachem H-1998), Allophycocyanin (APC)-labeled anti-hCD41 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteolytic activity was measured using Z-Arg-Leu-Arg-Gly-Gly-AMC substrate (Bachem, 369 I1690) as described previously in Ashhurst et al ...
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bioRxiv - Microbiology 2021Quote: Protease activity of recombinant Mpro was measured using the quenched fluorogenic substrate {DABCYL}-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu- (EDANS)-NH2 (Bachem, Vista, CA, USA). 5 μL of 25 nM Mpro diluted in assay buffer [25 mM HEPES (pH 7.4) ...
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bioRxiv - Molecular Biology 2024Quote: ... assay using a custom synthesized peptide substrate with (7-Methoxycoumarin-4-yl)acetyl [MCA] as fluorophore and 2,4-Dinitrophenyl [DNP] as fluorescence quencher: MCA-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lsy-NH2-trifluoroacetate salt (Bachem AG, Bubendorf CH). This peptide substrate amino acid sequence corresponds to the nsp4/nsp5 (Mpro ...
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bioRxiv - Biophysics 2023Quote: ... as fluorophore and 2,4-Dinitrophenyl (DNP) as fluorescence quencher: MCA-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lsy-NH2-trifluoroacetate salt (Bachem AG, Bubendorf CH). This peptide substrate amino acid sequence corresponds to the nsp4/nsp5 (3CLpro ...
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bioRxiv - Bioengineering 2023Quote: ... A peptide derived from the extracellular Ig domain of rabbit CD47 (Ac-Gly-Asn-Tyr-ThrCys-Glu-Val-Thr-Glu-Leu-Ser-Arg-Glu-Gly-Lys-Thr-Val-Ile-Glu-Leu-Lys-LINKER-Cys-OH) was custom synthesized by Bachem (Torrance, CA, USA). A terminal reactive cysteine residue was attached to the pepCD47 core sequence through a dual hydrophilic spacer (AEEAc-AEEAc) ...
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bioRxiv - Biochemistry 2021Quote: ... a fluorogenic MPro substrate DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS from Bachem, and K777 as a gift from Prof ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We acquired Sub3 with the sequence as DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS from Bachem Inc.
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bioRxiv - Microbiology 2021Quote: ... reaction was started without further incubation by adding the substrate Boc-Leu-Arg-Arg-AMC (Bachem. Bubendorf BL ...
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bioRxiv - Immunology 2021Quote: ... and incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM ...
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bioRxiv - Biochemistry 2022Quote: ... Residual uPA activity was assayed by the rate of proteolysis of the fluorescent substrate Z-Gly-Gly-Arg-AMC (Bachem) over a 10 min time course (λem = 370 nM ...
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bioRxiv - Biochemistry 2024Quote: ... For human trypsin (PH5.1) and human thrombin (PH5.3) we used the fluorogenic substrate Z-Gly-Gly-Arg-AMC (Bachem, Bubendorf, Switzerland), while for human plasmin (PH5.2 ...
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bioRxiv - Microbiology 2020Quote: The proteolytic activity of NS2B-NS3pro was measured using a fluorescence resonance energy transfer-based assay with a fluorogenic peptide substrate (Boc-Gly-Arg-Arg-AMC, No: I-1565, Bachem) as the substrate ...
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bioRxiv - Biochemistry 2021Quote: ... Plasma was incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Gly-ol5]-enkephalin (DAMGO; H-2535, Bachem), GABAA receptor antagonist picrotoxin (PTX ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorogenic peptide Mca-Pro-Leu-OH (Bachem, M-1975) was used as calibration standard to obtain the molarity (pmol ...
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bioRxiv - Biochemistry 2022Quote: ... All assays were performed with 75 ng enzyme on 96-well plates (BIOFIL®) in purification butter supplemented with 0.3 mM h-Gly-Pro-βNA (BaChem) at 30 °C ...
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bioRxiv - Neuroscience 2022Quote: ... or C (H-Gly-Phe-AMC, 56 μM, Bachem) in an assay buffer comprising 150 mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... inhibition of NOTCH signalling in P1 organoids was achieved by adding either 50 µM [(2R,4R,5S)-2-benzyl-5-(Boc-amino)-4-hydroxy-6-phenyl-hexanoyl]-Leu-Phe-NH2 trifluoroacetate salt (L-685,458; Bachem) or 10 µM Deshydroxy LY-411575 (DBZ ...
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bioRxiv - Biochemistry 2020Quote: ... and H-Glu(Thz-Gly-OH)-OH trifluoroacetate) were synthesized by Bachem (UK) Ltd ...
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bioRxiv - Cell Biology 2023Quote: ... Pyr-Arg-Thr-Lys-Arg-AMC (1 mM, Bachem) was prepared in 100 mM HEPES buffer (pH 7.0) ...
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bioRxiv - Microbiology 2021Quote: ... Suc-Leu-Leu-Val-Tyr-AMC (Bachem, Bubendorf BL, Switzerland) was used as a substrate at a final concentration of 52.5 μM.55 Proteolytic activity of matriptase-2 was measured in a final concentration of 2.5 nM Enzyme in 180 μL reaction buffer (50 mM TRIS-HCl (pH 8.0) ...
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bioRxiv - Systems Biology 2022Quote: ... a fluorogenic substrate was used (Suc-Leu-Leu-Val-Tyr-AMC, Bachem ## I-1395 ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide substrate Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC) (Bachem) was added at 10 µM to 10 µg of total protein ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) with 0.05 mM substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem). ChemiDoc MP (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... One unit of activity corresponds to the number of micromoles of Z-Arg-Arg-pNA (BACHEM) digested per min per mg of protein.
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bioRxiv - Biochemistry 2023Quote: ... the Z-Phe-Arg-AMC (Bachem) substrate was at 50 µM concentration in reaction buffer composed of 20 mM MES pH 6.0 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bz-Phe-Val-Arg-AMC.HCl (Thrombin substrate III) and Boc-Gln-Ala-Arg-AMC.HCl (Trypsin substrate) were from Bachem, Suc-Ala-Ala-Pro-Phe-AMC (Chymotrypsin substrate II ...
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bioRxiv - Pathology 2022Quote: ... 1 mM DTT) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) for 30 min at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... 1 mM MgCl2) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM ATP) supplemented with 100 mM of Suc-Leu-Leu-Val-Tyr-AMC (Bachem, Switzerland) substrate for chymotrypsin-like activity of 20S CP ...
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bioRxiv - Biochemistry 2024Quote: ... 40 µg z-Phe-Arg-pNA (Bachem) in 150 µl CtsK digestion buffer (100 mM sodium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... while for human plasmin (PH5.2) we used the fluorogenic substrate H-D-Val-Leu-Lys-AMC (Bachem, Bubendorf, Switzerland). All fluorogenic substrates were used at final concentration of 50 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Crustacean cardioactive peptide (CCAP: PFCNAFTGC-NH2; Bachem) was similarly dissolved into DDI-H2O to create a stock solution (10−3 M) ...
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bioRxiv - Microbiology 2021Quote: Cbz-Phe-Arg-AMC (Bachem, Bubendorf BL, Switzerland) was used as a substrate for Rhod ...
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bioRxiv - Cell Biology 2023Quote: ... Z-Phe-Arg-AMC HCl (1 mM, Bachem) was prepared in 50 mM Tris (pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... γ-Glu-Cys and Cys-Gly were from BACHEM Peptides (Germany) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... gly-ol] enkephalin acetate (DAMGO) (Bachem and Sigma-Aldrich), etonitazene hydrochloride fentanyl citrate ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl amoeba extract and 50 µl of the synthetic peptide Z-Arg-Arg-pNA (400 µM concentration; Bachem, Bubendorf, Switzerland) as substrate ...
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bioRxiv - Neuroscience 2020Quote: ... The competitor ligands were OT (CYIQNCPLG-NH2 ; Bachem), AVP (CYFQNCPRG-NH2 ...
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bioRxiv - Immunology 2024Quote: ... 500 μM Q-peptide (Ac-NQEQVSPLGGERCG-NH2) (Bachem) and 1 mM RGD (Ac-RGDSPGERCG-NH2 ...
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bioRxiv - Immunology 2024Quote: ... and 1 mM RGD (Ac-RGDSPGERCG-NH2) (Bachem). The other solution contained a 7.5 mM di-cysteine modified Matrix Metalloprotease (MMP ...
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bioRxiv - Cell Biology 2023Quote: ... Boc-Gln-Ala-Arg-AMC HCl (2.5 mM, Bachem) was prepared in 50 mM Tris (pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Boc-Gln-Ala-Arg-AMC·HCl (Bachem, Torrance, CA. USA) was dissolved in DMSO and diluted in reaction buffer (50 mM Tris pH 8 ...
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bioRxiv - Neuroscience 2021Quote: ... or 0.2 μg (Thr⁴,Gly⁷)-Oxytocin(TGOT) (BACHEM, lot #1062174) or 0.25 μg of Vasopressin (Phoenix Pharmaceuticals Inc. ...
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bioRxiv - Physiology 2023Quote: We assessed the in vitro plasma disappearance of GLP-1(7–36)NH2 and GLP-1(9–36)NH2 (Bachem, Switzerland) independent of use of inhibitors ...
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bioRxiv - Cell Biology 2024Quote: GLP-1(7-36)NH2 was purchased from Bachem; semaglutide was obtained from Imperial College London Healthcare NHS Trust pharmacy ...