Labshake search
Citations for Pall :
1 - 50 of 63 citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... gel electrophoresis and transferred to PVDF membrane (Pall laboratory) by semi-wet blotting.
-
bioRxiv - Systems Biology 2021Quote: ... mixed by pipette and transferred to 96 well nucleic acid binding plates (Pall). After washing with 500 μL Buffer AW1 and AW2 (Qiagen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein were separated on 12% denaturing polyacrylamide gel electrophoresis (PAGE) gels and transferred to a nitrocellulose membrane (Pall Biosciences). The analysis of these proteins was conducted using specific antibodies.
-
bioRxiv - Systems Biology 2021Quote: ... 200μL of 100% ethanol (Koptec) was added to each sample before mixing and samples were transferred to a Nucleic Acid Binding (NAB) plate (Pall) on a vacuum manifold with a 96DW collection plate ...
-
bioRxiv - Systems Biology 2020Quote: ... 200 μL of 100% ethanol (Koptec) was added to each sample before mixing and samples were transferred to a Nucleic Acid Binding (NAB) plate (Pall) on a vacuum manifold with a 96DW collection plate ...
-
bioRxiv - Microbiology 2020Quote: ... Cells for measuring nucleic acids were collected similarly on 0.22 µm Supor-200 Membrane Disc Filters (25 mm; Pall Corporation) and preserved in storage buffer (40 mM EDTA ...
-
bioRxiv - Systems Biology 2023Quote: ... 200 μL of 100% ethanol (Koptec) was added to each sample before mixing and samples were transferred to a nucleic acid binding (NAB) plate (Pall) on a vacuum manifold with a 96DW collection plate ...
-
bioRxiv - Microbiology 2021Quote: ... Approximately 1 L of seawater was prefiltered using an 85 μm Nitex mesh and subsequently collected on a 25-mm diameter 0.1-μm pore-sized polyethersulfone (PES) membrane for nucleic acids (Supor-100, Pall Gelman Inc., Ann Arbor, MI). The filters were submerged in DNA lysis buffer [40,41] and stored at −80°C until extraction.
-
bioRxiv - Cancer Biology 2022Quote: ... Extracted proteins were analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis and then transferred onto a polyvinylidene fluoride membrane (Pall). Western blotting was performed using a polyclonal anti-ghrelin primary antibody (1:250 ...
-
bioRxiv - Neuroscience 2023Quote: ... Then proteins were loaded on 6% or 8% sodium dodecyl sulphate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose filter membrane (PALL). Non-specific binding sites were blocked with Tris-buffered saline-Tween (0.02 M Tris ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein extracts (20 µg for brain and 40 μg for LMMP per lane) were subjected to electrophoresis (10% (vol/vol) SDS-polyacrylamide gel) and electroblotted onto nitrocellulose membranes (0.2 μm; Pall Life Science). Immunodetection was accomplished by enhanced chemiluminescence (ECL ...
-
bioRxiv - Cell Biology 2021Quote: Whole cell extracts were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on SuperSep™ Ace 5-20% gradient gels (Wako Pure Chemical Industries) and electrotransferred to nitrocellulose membranes (Pall Corporation). Membranes were blocked in Blocking One (Nacalai Tesque ...
-
bioRxiv - Cell Biology 2021Quote: ... Fifty micrograms protein per sample was loaded in each lane and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Pall Corporation, USA) in Tris-glycine buffer (48 mM Tris ...
-
bioRxiv - Neuroscience 2022Quote: Equal amounts of protein (100 μg) were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to a PVDF membrane (Pall Co., Taiwan) that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) and transferred to a BioTrace™ polyvinylidene fluoride membrane (Pall Corporation, New York, NY, USA) using a semi-dry blotting system (PEQLAB ...
-
bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Cat. #BSP0161; Pall Corporation, New York, NY, USA). The membranes were blocked with 5% non-fat milk and incubated with the following antibodies ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Gel extraction by diffusion at 65°C and gel filtration (NanoSep, Pall Life Sciences) followed by Agencourt AMPure bead purification was used to generate the final fully purified fragments ...
-
bioRxiv - Cell Biology 2021Quote: ... Gels were electroeluted onto Nitrocellulose (BioTrace NT, Pall) 1.2 mA/cm2 for 1 hr at RT under semi-dry blotting conditions (25 mM Tris ...
-
bioRxiv - Cell Biology 2020Quote: ... Gels were transferred to a nitrocellulose membrane (PALL), and subsequently incubated with indicated primary and secondary antibodies in 5% skim-milk Tris-buffered saline solution with 0.1% Tween 20.
-
bioRxiv - Microbiology 2021Quote: ... Ten micrograms of RNA were separated on a denaturing 8% polyacrylamide gel or a denaturing 1% agarose gel and transferred to a nylon membrane (Hybond N+ membrane or Pall membrane). For hybridisation experiments radioactively labeled PCR probes against the desired targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... Gels were then transferred to FluoroTrans PVDF Membranes (Pall). For antibody staining ...
-
bioRxiv - Genetics 2020Quote: ... The gels were transferred to Biodyne B membranes (Pall) for Southern hybridization ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were then transferred to BioTrace NT nitrocellulose paper (Pall Corporation #66485) for 200 minutes at 200 mAmps at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins on the gels were transferred to FluoroTrans W PVDF Transfer Membranes (Pall; 90 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... separated on a 1% agarose gel and transferred onto a nylon membrane (Pall membrane). For hybridisation experiments radioactively labeled PCR probes against the selected targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies ...
-
bioRxiv - Genetics 2020Quote: ... The gel slurry was transferred to a 0.45 μm NanoSep MF Tube (Pall Lifesciences) and centrifuged at maximum speed for 5 min at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Proteins in the gels were then blotted onto to a PVDF membrane (BioTrace, PALL) at 150 V for 90 min in ice-cold transfer buffer (1:5 volume of 100% Methanol in TGS buffer) ...
-
bioRxiv - Bioengineering 2021Quote: ... Both sample and standards were brought up to 55 μl with 0.1% formic acid and purified on 3.5 MWCO/Nanosep 3K spin columns (Pall) for 10 minutes at 14,000 RCF ...
-
bioRxiv - Biochemistry 2020Quote: ... the sample was again dried and subsequently resuspended in solvent A (0.1% formic acid in water) and passed through a 0.2 μm filter (Nanosep, PALL) prior to analysis by liquid chromatography-tandem mass spectrometric analysis (LC-MS/MS).
-
bioRxiv - Biochemistry 2023Quote: ... Biomass was collected after 24hr by vacuum filtering each incubation sample at 34.5 kPa (5 psi) onto an acid-cleaned 3μm pore-size acrylic copolymer Versapore filter (Pall) mounted on an acid-cleaned plastic filtration rig ...
-
bioRxiv - Plant Biology 2020Quote: Proteins separated on SDS polyacrylamide gels were transferred onto 0.2 μm nitrocellulose membrane (Pall Corporation). The blotted membrane was blocked with 5% (w/v ...
-
bioRxiv - Biophysics 2022Quote: Sandwich the gel between two sheets of DNA/RNA transfer membrane (Pall BiodyneB 0.45μm #60207). Sandwich the membrane-gel-membrane assembly between two sheets of Whatman paper (GE Healthcare #3030-917) ...
-
bioRxiv - Molecular Biology 2020Quote: The micro bicinchoninic acid (BCA) protein assay kit and Nanosep® 30K Omega Centrifugal Devices were purchased from Pall Life Sciences (Ann Arbor MI ...
-
bioRxiv - Biochemistry 2019Quote: ... peptides were dried down and reconstituted with 5% acetonitrile in 0.1% formic acid and spun through a 0.2um bio-inert membrane tube (PALL, ODM02C35) prior to autosampler tube transfer ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were resolved by 12.5% SDS-PAGE gel and then transferred to nitrocellulose membranes (Pall Corporation). Membranes were blocked with 5% non-fat milk in TBST (0.1% Tween 20 ...
-
bioRxiv - Microbiology 2020Quote: ... proteins in the gel were transferred to a pure nitrocellulose blotting membrane (Pall® Life Sciences). The membrane with proteins was first blocked with PBST (0.1 % Tween-20/PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were resolved in 7.5% SDS-PAGE gels and transferred to PVFD membranes (Pall Corporation, PVM020C099). The membrane was stained with Ponceau-S solution (PanReac AppliChem ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in 7.5% SDS-PAGE gels and transferred to PVFD membranes (Pall Corporation, PVM020C-099). For protein phosphorylation states ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 μL of 0.1 % formic acid (FA) was added to the digested sample and then loaded to a 10-kDa-size exclusion membrane (PALL, Nanosep), and centrifuged at 15,000 x g for 5 min to filter out peptides ...
-
bioRxiv - Cell Biology 2019Quote: ... Released sialic acids were isolated by spin-filtration using a 3,000 kDa molecular weight cutoff centrifugal device (Nanosep 3K Omega, PALL Life Sciences, OD003C34). The flow-through containing released sialic acid was dried and derivatized using 1,2-diamino-4,5-methylene-dioxybenzene (DMB ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysates (10 μg each) were separated on 7.5% polyacrylamide gels and transferred on to PVDF membranes (Pall). Then the membranes were incubated in Blocking One (Nacalai Tesque ...
-
bioRxiv - Systems Biology 2023Quote: The slices were transferred from the gel-slicing tool to a 96-well glassfiber (GF) filter plate (Pall), which contained 200 µL H2O ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were resolved in general in 7.5% SDS-PAGE gels and transferred to PVFD membranes (Pall Corporation, PVM020C099). The membrane was stained with Ponceau S solution (PanReac AppliChem ...
-
bioRxiv - Physiology 2020Quote: ... were resolved on an 8% native gel and proteins were transferred onto PVDF membranes (Pall Canada, Mississauga, ON, Canada) by a semi-dry transfer system and stained with Ponceau S ...
-
bioRxiv - Neuroscience 2022Quote: ... About 40 µg total protein of the supernatants were fractioned on 8–20% precast protein gels and transferred to 0.2 µm pore-size nitrocellulose membranes (66485, Pall). For monomer Aβ detection ...
-
bioRxiv - Molecular Biology 2019Quote: ... Ten µg RNA was separated on a 1.5% agarose gel and subsequently transferred to a nylon membrane (Biodyne® A, PALL). After UV-crosslinking the membrane was hybridised with a radioactively labelled probe against the 5’-part of the bgaHa mRNA to detect termination events ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein samples were separated in a 10% SDS-PAGE gel and transferred to a PVDF membrane (66543, Pall Gelman Laboratory). Immunodetection of OsNAC2 ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins were separated in 7.5% SDS-polyacrylamide gels (SuperSep™ Ace; Fujifilm) and transferred to polyvinylidene fluoride membranes (FluoroTrans W; Pall) with EzFastBlot transfer buffer (ATTO ...
-
bioRxiv - Cancer Biology 2020Quote: ... and equal amount of proteins were loaded into a 6%-15% SDS-polyacrylamide gradient gel and transferred onto nitrocellulose membrane (BioTraceNT; Pall Corp.). Membranes were washed in Tris Buffer Saline with 0.1% Tween 20 (TBS-T ...
-
bioRxiv - Plant Biology 2020Quote: 10 μL each of the thylakoid membrane proteins extracted from blue native gel bands was spotted on a nitrocellulose membrane (Pall Corporation). After spots on the membrane were completely air-dried ...