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Citations for Pall :
101 - 150 of 221 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... were resuspended in 500 μL of sterilized water in a 96-well filter plate (1 mL well, 0.45 μm Supor membrane, PALL, #8129). Then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and transferred 900 μl of the supernatants to AcroPrep Advance 96-well 1 ml filter plates (with a 0.2 μm supor membrane; Pall Corporation, USA), attached to an autoclaved 1.2 ml 96-well PCR plate (VWR) ...
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bioRxiv - Bioengineering 2022Quote: The total volumes of 1 L of influent and effluent were filtered through 0.22 μm polyvinylidene fluoride (PVDF) membranes (Pall Corporation, USA). The filtrates were used to isolate the exDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... and separated on a 4% (v/v) polyacrylamide (29:1) gel containing 8 M urea and transferred to Biodyne nylon membrane (Pall Corporation) at 400 mA for 1 hour in 0.5× TBE buffer ...
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bioRxiv - Microbiology 2021Quote: ... Ten micrograms of RNA were separated on a denaturing 8% polyacrylamide gel or a denaturing 1% agarose gel and transferred to a nylon membrane (Hybond N+ membrane or Pall membrane). For hybridisation experiments radioactively labeled PCR probes against the desired targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... samples were centrifuged at 14,000 g for 1 min and 300 µL supernatant was transferred into Omega 3K MWCO AcroPrep 96-well filter plates (Pall, USA). After centrifuging for 45 min at 3,000 g ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The insoluble fraction of the lysate was removed via centrifugation through 1 mm glass fiber Acroprep Advance 96-well filter plates (8031, Pall, USA). The soluble flow-through fraction was analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess dye and other reagents were removed using solid phase extraction (SPE) with an Acroprep 1 mL 0.2 μm wwPTFE plate (Pall, NY, USA) used as stationary phase (Pucic et al. ...
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bioRxiv - Plant Biology 2020Quote: Proteins separated on SDS polyacrylamide gels were transferred onto 0.2 μm nitrocellulose membrane (Pall Corporation). The blotted membrane was blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... biotinylated SARS-CoV-2 S trimer protein was loaded onto streptavidin (SA) biosensors (Pall FortéBio). S-trimer-bound biosensors were dipped into wells containing varying concentrations of ACE2 protein and the interactions were monitored over a 500-sec association period ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were resolved by SDS/PAGE and transferred to 0.22 um nitrocellulose membrane (PALL), which then was incubated overnight with primary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Protein (100 μg) was loaded onto 10-kD cutoff filter devices (Pall, Port Washington, NY) and washed sequentially with UA (8 mol/L urea ...
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bioRxiv - Microbiology 2021Quote: ... and tag-free proteins were collected and concentrated using a 10K MWCO centrifugal filter (PALL). Concentrated proteins were further purified by size exclusion chromatography using Superdex 75 Increase 10/300 GL (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were stained by Brilliant Blue Coomassie or transferred to nitro-cellulose membranes (Pall Corporation) for immunodetection with custom-made α-FLVA ...
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bioRxiv - Molecular Biology 2024Quote: ... Secreted proteins were concentrated and exchanged into PBS using a tangential flow filtration cassette (PALL), then purified by Strep-Tactin Sepharose resin (IBA Lifesciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transferred to Hybond Nx nylon membranes (Amersham) and crosslinked using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Sigma) (Pall & Hamilton 2008). Membranes were pre-hybridized in Ultrahyb Oligo hybridization buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... The adjusted supernatant was loaded into a pre-packed CMM HyperCel column (1-mL or 5-mL) (Pall Corporation, Port Washington, NY), re-equilibrated with 20 mM sodium citrate pH 4.5 ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μg of the protein sample was loaded on the 10-kD filter unit (Pall, USA). The protein solution was reduced with 4.5 mM DTT for 1 h at 37°C and then alkylated with 10 mM of indoleacetic acid for 30 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... 100 µg of proteins was loaded onto 10-kD cutoff filter devices (Pall, Port Washington, NY) and washed twice with UA (8 M urea in 0.1 M Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were concentrated using Microsep™ Advance Centrifugal Devices 10,000 MWCO centrifugal filter units (PALL) and were stored at −80°C following flash-freezing in liquid nitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Denatured proteins were separated in 12% SDS–PAGE and then transferred onto nitrocellulose papers (Pall, USA). After blotting ...
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bioRxiv - Physiology 2020Quote: ... Proteins were then transferred on to BioTrace NT nitrocellulose membranes (Pall Life Sciences, Pensacola, Florida, USA) using a wet transfer system at 100 V for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The peak fractions were pooled and concentrated using Macrosep 20 mL 10K MWCO protein concentrators (Pall). Aliquots were flash-frozen in liquid nitrogen and stored at −80°C until use for crystallization or in preparation of hE-cadherin EC1-2-Activating Fab complexes.
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bioRxiv - Biophysics 2022Quote: ... Proteins were resolved using 8% SDS-PAGE and subsequently transferred to a nitrocellulose membrane (Pall Corporation). Membranes were probed with a rabbit polyclonal antibody against the C-terminal 19aa of IP3R1 (CT1 ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions were pooled and concentrated using Macrosep 20 mL 10K MWCO protein concentrators (Pall). Aliquots were flash-frozen in liquid nitrogen and stored at −80°C until use for preparation of hE-cadherin EC1-2-Activating Fab complexes.
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resolved by 12.5% SDS-PAGE gel and then transferred to nitrocellulose membranes (Pall Corporation). Membranes were blocked with 5% non-fat milk in TBST (0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... the protein solutions were concentrated by centrifugation (Spin filters 10k, Pall Laboratory, Port Washington, NY, USA), and their purities were analyzed by SDS- PAGE (MilliporeSigma).
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bioRxiv - Microbiology 2020Quote: ... proteins in the gel were transferred to a pure nitrocellulose blotting membrane (Pall® Life Sciences). The membrane with proteins was first blocked with PBST (0.1 % Tween-20/PBS ...
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bioRxiv - Microbiology 2022Quote: ... The separated proteins were transferred to a 0.2 μm PVDF membrane (Catalog no. BSP0161; Pall Corporation) for 1 h and 20 min at 90 V and 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Protein samples were resolved by SDS-PAGE and transferred onto a nitrocellulose membrane (Pall life sciences). Blocking the membrane was performed with 5% BSA (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were resolved in 7.5% SDS-PAGE gels and transferred to PVFD membranes (Pall Corporation, PVM020C099). The membrane was stained with Ponceau-S solution (PanReac AppliChem ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was cross-linked to the membrane (60°C, 1 h) using freshly prepared EDC cross-linking solution (Kim et al., 2010)(Pall et al., 2007). Pre-hybridization and hybridization were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... Aliquots of each final product were diluted five folds in PBS and then 1 μl was spotted on silica gel impregnated glass fibre sheets (PALL Life Sciences, UK). The strips were developed with a mobile phase of 50mM EDTA in 0.1M ammonium acetate and allowed to dry before analysis ...
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bioRxiv - Immunology 2020Quote: ... diluted in binding buffer (1:10 vol/vol, Tris 20 mM, pH 8.0, 150 mM NaCl) and filtered (PALL Lifesciences #4612, 0.2 µM). Samples were passed through the column (1 mL/min. ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were transferred to Hybond-NX membranes (Amersham) and crosslinked to the membranes with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Pall and Hamilton, 2008). Antisense probes were radiolabeled at their 5′ ends with [γ-32P] ATP by T4 polynucleotide (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... Sediment supernatant and water samples were concentrated by filtration through the Pall Envirochek™ 1 μm HV capsule filter (Pall South Africa (Pty) Ltd. ...
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bioRxiv - Microbiology 2024Quote: ... The glycans were preloaded onto streptavidin-coated biosensors at up to 0.3 µg/ml for 5 minutes in 1 × kinetic buffer (Pall FortéBio, Menlo Park, CA). Each test virus was diluted to a final concentration of 100 pM with 1 × kinetic buffer containing 10 µM oseltamivir carboxylate (American Radiolabeled Chemicals ...
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bioRxiv - Immunology 2021Quote: ... Supernatant containing RBD protein was then concentrated using a 10 kDa MW cutoff Centramate cassette (Pall Corporation) followed by affinity chromatography using Ni-NTA resin (QIAGEN ...
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bioRxiv - Plant Biology 2022Quote: ... the protein extract was concentrated over a tangential flow filtration (MinimateTM Tangantial Flow Filtration Systems, Pall Corporation) using a molecular weight cutoff of 1 kDa (i.e ...
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bioRxiv - Genomics 2019Quote: ... The DNA-protein complex was transferred on positively charged Biodyne nylon membrane (Pall Industries, Fort Washington, NY) using 0.5% TBE for 45 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... One hundred micrograms of protein was loaded onto 10-kD cutoff filter devices (Pall, Port Washington, NY). The samples were washed sequentially with UA (8 mol/L urea ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved in 7.5% SDS-PAGE gels and transferred to PVFD membranes (Pall Corporation, PVM020C-099). For protein phosphorylation states ...
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bioRxiv - Biochemistry 2022Quote: ... The urine protein was loaded on the filter membrane of a 10 kDa ultrafiltration tube (PALL, USA) and washed twice with UA (8 mol/L urea ...
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bioRxiv - Biochemistry 2023Quote: Protein samples for size-exclusion chromatography were filtered through Nanosep filters with 0.45 μm wwPTFE membrane (Pall) to avoid the presence of aggregates after the freeze and thaw cycle ...
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bioRxiv - Bioengineering 2019Quote: ... and the buffer exchanged with PBS containing 1 mM MgCl2 (PBSM) using a Microsep 10 kDa Omega centrifugation spin column (PALL Corp; Port Washington, NY). Purification was confirmed via SD-PAGE analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl of the affinity matrix was transferred to a 0.45 μm 96-wells filter plate (AcroPrep Advance 1 mL, PALL, New York, New York, USA). The affinity matrix was washed three times with 400 μl PBS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... samples were filtered using a 0.2 μM low protein-binding filter with a HT Tuffryn Membrane (Pall Corporation). Samples were injected into a High Precision Quartz SUPRSIL cuvette with 0.1 cm pathlength (Hellma Analytics) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein in each sample was loaded into a 10-kDa filter device (Pall, Port Washington, NY, USA). After washing two times with urea buffer (UA ...
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bioRxiv - Cell Biology 2021Quote: ... Fifty microliter of supernatant was saved for protein assay (BCA assay, Pierce) while the rest was filtered (Pall Nanosep® OD003C33 centrifugal device with 3K Omega membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The protein in each sample was loaded into a 10-kDa filter device (Pall, Port Washington, NY, USA). After washing two times with urea buffer (UA ...