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Citations for Molecular Cloning Laboratory :
1 - 50 of 73 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Glutathione agarose beads (MCLAB # GAB-300) were equilibrated with wash buffer (50 mM Tris HCl pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were purified (BioBasic, USA) and sent for sequencing (Molecular Cloning Lab, USA). The correct acr-8 clone was then transferred to the pTD2 expression vector ...
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bioRxiv - Biophysics 2024Quote: ... 0.2 mg/mL acetylated BSA (Molecular Cloning Laboratories), 0.0025% P-20 surfactant (GE healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µL 2X HotStart PCR Master Mix (HMM, MCLAB), 0.7 µL SYTO9 and mouse primer pairs topped up with nuclease-free H2O ...
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bioRxiv - Microbiology 2024Quote: ... The integrity of all constructs was confirmed by sequencing (McLab).
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bioRxiv - Biophysics 2024Quote: ... DNA sequences confirmed with sequencing from Molecular Cloning Labs.
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bioRxiv - Biochemistry 2024Quote: ... Cleavage reactions were analyzed by capillary electrophoresis on an Applied Biosystems 3130xl instrument equipped with a 36 cm capillary array and nanoPOP-6 polymer (Molecular Cloning Laboratories, San Francisco CA) as previously described252523 ...
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bioRxiv - Synthetic Biology 2024Quote: ... insertion regions were verified by Sanger sequencing (McLAB or Azenta/Genewiz). For larger inserts ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and confirmed by Sanger sequencing (McLab Sequencing, San Francisco, CA). Chemically competent E ...
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bioRxiv - Biophysics 2024Quote: ... coli single-strand DNA binding protein (SSB) and hnRNP K were obtained commercially from MCLAB and LSBio ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were then applied to 100 μL polylysine magnetic beads (Molecular Cloning Laboratories) for 15-30 minutes at 4 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were extracted from these overnight cultures using the homemade miniprep kit and subsequently validated by Sanger sequencing (McLab) or NGS sequencing (Plasmidsaurus).
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bioRxiv - Biochemistry 2024Quote: ... equipped with a 36 cm capillary array and nanoPOP7 polymer (MCLab)37 ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was incubated with nickel-nitrilotriacetic acid (NiNTA) resin (MCLAB) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... or 100U Bst LF polymerase (McLab #BPL-200). After the 15 min preincubation 1 µM of oligo pool mix was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific, USA) and sequence verified (McLab, USA). All sequences were cloned in the pTD2 oocyte expression vector which contains the gene of interest flanked by 3’- and 5’- X ...
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bioRxiv - Molecular Biology 2023Quote: ... and transformed into DH5α (ThermoScientific, USA) and sequence verified (McLab, USA).
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bioRxiv - Microbiology 2023Quote: ... and MC1061 (Molecular Cloning Laboratories #MC1061), Staphylococcus aureus ...
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bioRxiv - Microbiology 2023Quote: ... coli MC1061 strain (Molecular Cloning Laboratories #MC1061) was used as the cloning host for all pTRK892-based plasmids.
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bioRxiv - Synthetic Biology 2023Quote: ... All sensor and ligand proteins except for SH2 were purified by nickel-nitrilotriacetate chromatography according to the manufacturer’s instructions (Molecular Cloning Laboratories). SH2 did not have a His-Tag and was purified using an SP Sepharose column as described37 ...
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bioRxiv - Genomics 2023Quote: ... Element insertion was confirmed by Sanger sequencing (MCLAB). Each element was individually prepped 3 times for a total of 6 individual plasmid preparations per nominated region ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated chimeras were transformed into DH5α (ThermoScientific, USA) and sequence verified (McLab, USA).
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bioRxiv - Microbiology 2023Quote: ... followed by verification and species identification using Sanger Sequencing (Molecular Cloning Lab). Phylogenetic trees were created using Phylogeny.fr23 based on ITS sequences ...
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bioRxiv - Molecular Biology 2023Quote: Conversion of dsDNAs to ssDNAs was determined by their binding to SSB (MCLAB - Molecular Cloning Laboratories, CA). DNA samples were mixed with 1 ...
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bioRxiv - Molecular Biology 2023Quote: Conversion of dsDNAs to ssDNAs was determined by their binding to SSB (MCLAB - Molecular Cloning Laboratories, CA). DNA samples were mixed with 1 ...
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bioRxiv - Genetics 2022Quote: ... PCR amplification of the genomic DNA from fish tail clips was also sent for sequencing (Molecular Cloning Laboratories, MCLAB, https://www.mclab.com/home.php). The sequencing primer sequences used to verify successful editing are provided in Supplemental Table 1.
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bioRxiv - Genetics 2022Quote: ... PCR amplification of the genomic DNA from fish tail clips was also sent for sequencing (Molecular Cloning Laboratories, MCLAB, https://www.mclab.com/home.php). The sequencing primer sequences used to verify successful editing are provided in Supplemental Table 1.
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bioRxiv - Biophysics 2022Quote: ... 1 mg/ml ultrapure Bovine Serum Albumin (BSA; MCLAB, UBSA-100) in F-buffer was injected and incubated for 2 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Circular form of circRNA was confirmed by resistance to the RNaseR (McLab) treatment (10 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were loaded onto a Ni-NTA column (MCLAB), washed with 25 mM imidazole and eluted in 300 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... All the gene sequences were verified by sequencing (MCLab, San Francisco, CA). The pET11a-hSUMO1 was kindly provided by Fauke Mechior (Addgene plasmid #53138).
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bioRxiv - Bioengineering 2022Quote: ... LwaCas13a proteins were purchased from MCLAB (cat# CAS13a-100). Cas13a and crRNA were mixed in 1×PBS to form the non-activated Cas13a/crRNA at room temperature for 20 min and stored at -80°C ...
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bioRxiv - Genomics 2022Quote: ... Element insertion was confirmed by Sanger sequencing (MCLAB). Each element was individually prepped 3 times for a total of 6 individual plasmid preparations per nominated region.
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bioRxiv - Genomics 2022Quote: ... 0.5uL of BST enzyme (McLab, BPL-300), and 44.5uL water (see Table S9 for oligo sequences) ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing the desired protein were pooled and mixed with SUMO Protease (MCLAB, CA, USA) with the provided SUMO buffer (salt-free) ...
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bioRxiv - Molecular Biology 2022Quote: ... reciprocal exchange through ligation and confirmed by sequencing (McLab, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... and the products were sequenced using Sanger sequencing by MCLab Molecular Cloning Laboratories (South San Francisco ...
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bioRxiv - Genetics 2021Quote: ... sent for Sanger sequencing by MCLAB (South San Francisco, California) or Quintara Biosciences (South San Francisco ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used for bi-directional Sanger sequencing on an ABI 3730 automated sequencer (MCLAB). Sequence chromatograms were imported and visualized using SEQUENCHER 5.2 (Gene Codes Corporation) ...
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bioRxiv - Immunology 2021Quote: ... and 0.1 µL ATP sulfurylase (MCLab). The reaction mixture was then incubated at 37°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... coli lysate (MCLAB #ECCL-100) was resuspended in MQ water and added to a final concentration of 15 µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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bioRxiv - Bioengineering 2021Quote: ... coli lysate (MCLab, ECCL-100) were prepared in 15 mM Na2CO3 ...
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bioRxiv - Biophysics 2021Quote: ... TEV protease (MCLAB #TEV-200) was added during dialysis at 10 μg/ml ...
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bioRxiv - Genomics 2021Quote: ... 1 µL of each synthetic sgRNA were incubated at room temperature with 1 µL of recombinant purified dCas9 (MCLab dCAS9B-200) for 20 minutes ...
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bioRxiv - Genomics 2021Quote: ... 6,250 ng of Cas9 (MCLAB CAS9-200) or dCas9 (MCLAB dCAS9B-200 ...
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bioRxiv - Genomics 2021Quote: ... followed by SPRI magnetic beads-based DNA extraction (MCLAB, MBC-200) using 80% ethanol for purification ...
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bioRxiv - Immunology 2021Quote: ... and 0.5 µL ATP sulfurylase (MCLab). The reaction mixture was then incubated at 37°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Quenched samples were analyzed by capillary electrophoresis on an Applied Biosystems 3130xl Genetic Analyzer instrument with a 36 cm capillary array filled with POP-6 polymer (MCLab). Before injection ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR product clean up and sequencing was done by Molecular Cloning Laboratories (www.mclab.com ...