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Citations for TriLink BioTechnologies :
1 - 50 of 55 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Developmental Biology 2023Quote: ... Cas9 (100 ng/µl) (TriLink Biotechnologies), gRNA (50 ng/µl each guide ...
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bioRxiv - Neuroscience 2022Quote: ... Cas9 mRNA (100 ng/uL. Trilink, L-6125) and both sgRNAs (100 ng/uL each ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 Nickase mRNA (Trilink; 100 ng/μl) in injection buffer ...
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bioRxiv - Genetics 2024Quote: ... and Cas9 Nickase mRNA (Trilink; 100 ng/μl) in injection buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... dRT (1.5-3 ng/μl) and Cas9 mRNA (100 ng/μl, Trilink Biotechnologies, San Diego, CA) was microinjected into pronuclei and cytoplasm of one-cell stage embryos obtained from superovulated B6D2F1 female mice (Jackson Laboratory ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixes containing 100 ng/µl Cas9 mRNA (5meC,Ψ) (TriLink BioTechnologies), 50 ng/µl sgRNAs and 50 ng/µl ssODN or 50 ng/µl lssDNA were prepared in microinjection buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... sgRNA sequences (20 ng/μL) and its corresponding donor oligonucleotide (100 ng/μL) were co-microinjected with Cas9 nickase mRNA (50 ng/μL, Trilink Biotechnologies) into the cytoplasm of zygotes collected from B6D2F1/J mice (JAX #100006) ...
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bioRxiv - Genetics 2023Quote: ... The sgRNA (20 ng/ul) and donor oligonucleotides (100 ng/ul) were co-microinjected with Cas9 mRNA (20ng/ul, purchased from Trilink Biotechnologies) into the cytoplasm of zygotes collected from C57BL/6N mice (Charles River Laboratory) ...
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bioRxiv - Synthetic Biology 2023Quote: ... along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink, N-7113). 5 mM N1-Methylpseudouridine-5’-triphosphate (Trilink ...
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bioRxiv - Immunology 2019Quote: ... Mice were generated by injection of a mixture of mammalian optimized Cas9 mRNA (100 ng/μl, TriLink Biotechnologies), purified gRNA_16 and gRNA_23 (50 ng/μl ...
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bioRxiv - Molecular Biology 2019Quote: Equal amounts of total RNA (100 ng) were used for small RNA library preparation using the CleanTag small RNA library preparation kit (TriLink Biotechnologies). Adapter-ligated libraries were reverse transcribed and amplified (19 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions testing IRES nLuc reporters were additionally supplemented with 50 ng/µL (final) competitor FFLuc mRNA (TriLink Biotechnologies # L-7602-100) to increase the fidelity of the IRES nLuc signal ...
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bioRxiv - Bioengineering 2023Quote: ... and m1Ψ-modified spike mRNA with di-proline substitutions of K968 and V969 were obtained from Trilink biotechnologies (San Diego ...
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bioRxiv - Immunology 2023Quote: ... a spike-in mRNA standard was added at this step (CleanCap EGFP mRNA from TriLink, L-7601) to account for RNA loss during processing ...
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bioRxiv - Molecular Biology 2023Quote: ... using the wget command (http://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_38/gencode.v38.transcripts.fa.gz) and the sequence for the eGFP spike-in (TriLink L-7601) was manually appended to the reference transcriptome (https://www.trilinkbiotech.com/media/folio3/productattachments/product_insert/egfporf_catno_l-7201_l-7601_l-7701_.txt).
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bioRxiv - Genetics 2021Quote: ... both sgRNAs (20 ng/µl each) and Cas9 mRNA (50 ng/µl, TriLink Biotechnologies, L-7206) were injected into the pronucleus and cytoplasm of fertilized eggs (Transgenic Animal Modeling Core ...
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bioRxiv - Cancer Biology 2023Quote: ... These sgRNAs (20 ng/μl each) and Cas9 mRNA (50 ng/ μl, purchased from Trilink Biotechnologies) were co-microinjected into the cytoplasm of zygotes collected from C57BL/6N mice (Charles River Laboratories) ...
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bioRxiv - Genetics 2019Quote: ... and Cas9 mRNA (25 ng/μL, TriLink) were co-injected into zygotes (F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J ...
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bioRxiv - Genetics 2019Quote: ... and Cas9 mRNA (25 ng/μL, TriLink) were co-injected into zygotes (F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J ...
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bioRxiv - Genetics 2023Quote: ... and Cas9 mRNA (25 ng/μL, TriLink) were co-injected into zygotes (F1 hybrids between strains FVB/NJ and B6(Cg)-Tyrc-2J/J ...
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bioRxiv - Neuroscience 2022Quote: ... or Cas9 mRNA (1000 ng/μl) (TriLink BioTechnologies), sgRNA (30 ng/μl ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 ng CleanCap Cas9 (5moU) mRNA (Trilink, L-7206) was electroporated into the cells using the Neon™ Transfection System (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: Base editor mRNA (evoCDAmax-SpCas9-NG) was provided by TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2023Quote: ... ABE8e-NG and BE4max were ordered as custom products from Trilink Biotechnologies ...
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bioRxiv - Bioengineering 2023Quote: ... 1.5 µL of 20 ng/µL Hpd-targeting sgRNA (Trilink Biotechnologies), and 4.9 µL of 61 µM SpCas9 V3 (Integrated DNA Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... A mixture of Cas9 mRNA (50 ng/ µl, #L-6125, TriLink Biotechnologies) and two specific gRNAs (25 ng/ µl each ...
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bioRxiv - Genomics 2022Quote: ... 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated 4-thiouridine (TriLink, N-1025). Different strands of pre-mRNA were generated for each construct that included different quantities of 4sU ...
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bioRxiv - Biophysics 2023Quote: ... and 100 µM GpA dinucleotides (TriLink) were added to the solution and incubated for additional 10 min at 37°C to allow the ternary complex to initiat transcription and stall at the first G in the template ...
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bioRxiv - Neuroscience 2023Quote: ... RNP complexes were supplemented with 500 ng EGFP mRNA (Trilink Biotechnologies, L-7201) per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 DL of 100 mM nonradioactive nucleotides [ppGpp (Trilink), ATP ...
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bioRxiv - Genetics 2020Quote: ... mice were microinjected with 50 ng/μl of wild type Cas9 mRNA (TriLink Biotechnologies, San Diego, CA), 50 ng/μl of sgRNA (Synthego Corp. ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Cancer Biology 2023Quote: ... The sgRNAs (20ng/ul each) were co-microinjected with Cas9 mRNA (50 ng/ul, purchased from Trilink Biotechnologies) into the cytoplasm of zygotes collected from B6D2F/J F1 hybrid mice (JAX Stock No ...
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bioRxiv - Bioengineering 2020Quote: ... 1 ng was used as template for initial error-prone PCR using 8-oxo-GTP and dPTP (TriLink Biotechnologies). Four 50 µl PCR reactions were performed on each template with increasing concentrations of mutagenic nucleotides (5 µM ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... Gene editing was performed by microinjection of RNA encoding the wild-type Cas9 nuclease (50 ng/μl, TriLink BioTechnologies, #L7606), 25 ng/μl single guide RNA (sgRNA ...
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bioRxiv - Cell Biology 2023Quote: ... and CpG 2 µg/ml (TriLink) were added in a final volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: ... was prepared in injection buffer by mixing 500 ng/μl of each of the sgRNAs (left and right) with Cas9 mRNA (1 μg/μl, TriLink, cat # L-6125). Fertilized eggs collected from B6/129 mice were microinjected at the CHOP transgenic core and transferred into pseudo-pregnant B6 females ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with the CleanCap® EGFP mRNA (#L-7601-100; TriLink Biotechnologies, San Diego, CA, USA) for the indicated times ...
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Bioengineering 2022Quote: ... however 2 μl of the appropriate canonical NTP (75 mM) was replaced by 2 μl of 100 mM 5-Hydroxymethyl-5’-triphosphate (e.g. 5-Hydroxymethyl-CTP) (TriLink).
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bioRxiv - Bioengineering 2023Quote: ... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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bioRxiv - Bioengineering 2023Quote: dAbs were generated by conjugating amine-modified oligonucleotides (15–25 OD260 units) to polyclonal antibodies (100 µg) using the Antibody-Oligonucleotide All-in-One Conjugation Kit from TriLink Biotechnologies (A-9202) ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA that was either purchased by TriLink (most experiments) or made in-house via in vitro transcription (IVT)40 at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Systems Biology 2019Quote: eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...