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Citations for TriLink BioTechnologies :
101 - 145 of 145 citations for 7 CHLORO 3 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Cell Biology 2021Quote: ... and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7701).
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bioRxiv - Immunology 2020Quote: ... Uridine 5’-triphosphate (UTP) was replaced with the triphosphate derivative of m1Ψ (m1ΨTP) (TriLink, San Diego, CA, USA Biotechnologies) in the transcription reaction ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at ∼70% confluency and reverse transfected with indicated concentrations of mRNA-Cas9-HA containing 5-methoxyuridine (5moU) or mRNA-eGFP 5moU (TriLink) in MessengerMAX (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Unmodified synthesized ANKRD55 mRNA transcript was capped at the 5’ end using wild-type bases CleanCap® AG (TriLink BioTechnologies) to generate a natural Cap 1 structure ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Immunology 2021Quote: ... 5′ amino-oligonucleotides were conjugated directly to each antigen using the SoluLINK Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Short mitochondrial DNA fragments (221bp) were amplified from gDNA from A549 cells with and without 8-Oxo-2’-deoxyguanosine-5’-Triphosphate (TriLink Biotechnologies) using GoTaq Mastermix ...
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bioRxiv - Immunology 2020Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 21-mer-sd or 21-mer-d 3′ and 14-mer 5′ ends vRNAs (IBA) were used in combination with synthetic 12-or 13-mer capped RNA (TriLink Biotechnologies) as primer (Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Bioengineering 2023Quote: ... synthesis of mRNA was carried out using MEGAscript™ T7 Transcription Kit (Waltham, MA, USA) with the addition of ACRA 5’ cap (Trilink biotechnologies) and m1Ψ-5’-triphosphate (Trilink biotechnologies ...
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bioRxiv - Microbiology 2020Quote: ... 2’-F-2’-dUTP (N-1010-1) and didanosine-TP (N-4017-1) were purchased from Trilink. Remdesivir-DP was synthesized by WuXi AppTech ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genes were targeted using two sgRNAs designed against regions at the 5’ end of each gene following the method outlined by Bassett et al.76 sgRNAs and Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) were injected into D ...
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 mM m1Ψ (TriLink, Cat#N-1019-1). The IVT reaction can be scaled down by a half ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP, TriLink Biotechnologies), 1 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: There were two sources for the mRNA used in this study: 1) commercial synthesis by TriLink Biotechnologies (TriLink mRNA was used in Fig ...
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bioRxiv - Immunology 2023Quote: ... for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies, # N-7113-1). For large-scale mRNA production ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Systems Biology 2019Quote: eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2020Quote: ... 1 ng was used as template for initial error-prone PCR using 8-oxo-GTP and dPTP (TriLink Biotechnologies). Four 50 µl PCR reactions were performed on each template with increasing concentrations of mutagenic nucleotides (5 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... with complete substitution of uridine to 1- methylpseudouridine and CleanCap AG analog (N-1081 and N-7113, TriLink Biotechnologies).
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bioRxiv - Synthetic Biology 2024Quote: ... transduced activation reporter cells were enriched by puromycin selection (1µg mL-1) following nucleofection of dCas9-VPR mRNA (TriLink) and a Cas9 sgRNA targeting the ESR protospacer (IDT) ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the single-site RosaBxb-GT host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired attP-GT carrier strains ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the dual-site RosaBxb-GT/GA host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired strain ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 104 cells were harvested and incubated in 10 ml Neon Buffer R with 1 ul (1ug/ul) of Clean-Cap Cas9 mRNA (TriLink Biotechnologies) and 1 ul (100 pmol/ul ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) and placed in the 20µl cuvette provided and CB-150 was the Amaxa protocol used ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were electroporated with 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) codon optimized ABE7.10 mRNA (TriLink Biotechnologies ...
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bioRxiv - Immunology 2023Quote: ... was prepared in injection buffer by mixing 500 ng/μl of each of the sgRNAs (left and right) with Cas9 mRNA (1 μg/μl, TriLink, cat # L-6125). Fertilized eggs collected from B6/129 mice were microinjected at the CHOP transgenic core and transferred into pseudo-pregnant B6 females ...
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bioRxiv - Immunology 2023Quote: ... The adjuvanted microsphere vaccine formulation contained 3 µg/mg of survivin-specific class 1 and class 2 peptides and 0.5 µg/mg of the TLR-9 oligonucleotide agonist CpG (ODN-1018) (Trilink Biosciences, San Diego, CA, USA). The microspheres ...