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Citations for TriLink BioTechnologies :
101 - 150 of 178 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: The 5’-triphosphorylated 29-nt RNAs used for capping reactions were synthesized by Trilink. The 22- ...
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bioRxiv - Synthetic Biology 2023Quote: ... along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink, N-7113). 5 mM N1-Methylpseudouridine-5’-triphosphate (Trilink ...
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bioRxiv - Biophysics 2019Quote: ... transcription was chased by adding 40 µM NTPs mix together with 50 µM of each type of 3’-deoxynucleotide RNA chain terminators (3’dATP, 3’dCTP, 3’dGTP, 3’dUTP, TriLink Biotechnologies). The reactions were allowed to proceed at room temperature for 10 min before terminated by adding the 2× urea stop buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 mM 5-methylcytidine triphosphate and 7.5 mM pseudo-uridine triphosphate (TriLink Biotechnologies, San Diego, CA). Transfections of modRNA and multiple siRNA targeted against Sirt1 and Satb2 (both from Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription in the presence of a mixture of 5’-biotin-G-monophosphate (TriLink Biotechnologies) and GTP (molar ratio 3:2 ...
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bioRxiv - Systems Biology 2023Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Genomics 2024Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Cell Biology 2021Quote: ... and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7701).
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bioRxiv - Bioengineering 2023Quote: ... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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bioRxiv - Immunology 2020Quote: ... Uridine 5’-triphosphate (UTP) was replaced with the triphosphate derivative of m1Ψ (m1ΨTP) (TriLink, San Diego, CA, USA Biotechnologies) in the transcription reaction ...
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bioRxiv - Bioengineering 2023Quote: ... was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA that was either purchased by TriLink (most experiments) or made in-house via in vitro transcription (IVT)40 at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at ∼70% confluency and reverse transfected with indicated concentrations of mRNA-Cas9-HA containing 5-methoxyuridine (5moU) or mRNA-eGFP 5moU (TriLink) in MessengerMAX (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Unmodified synthesized ANKRD55 mRNA transcript was capped at the 5’ end using wild-type bases CleanCap® AG (TriLink BioTechnologies) to generate a natural Cap 1 structure ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Molecular Biology 2022Quote: ... 21-mer-sd or 21-mer-d 3′ and 14-mer 5′ ends vRNAs (IBA) were used in combination with synthetic 12-or 13-mer capped RNA (TriLink Biotechnologies) as primer (Table 2 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Immunology 2023Quote: ... The adjuvanted microsphere vaccine formulation contained 3 µg/mg of survivin-specific class 1 and class 2 peptides and 0.5 µg/mg of the TLR-9 oligonucleotide agonist CpG (ODN-1018) (Trilink Biosciences, San Diego, CA, USA). The microspheres ...
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bioRxiv - Bioengineering 2023Quote: ... synthesis of mRNA was carried out using MEGAscript™ T7 Transcription Kit (Waltham, MA, USA) with the addition of ACRA 5’ cap (Trilink biotechnologies) and m1Ψ-5’-triphosphate (Trilink biotechnologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 mM Anti Reverse Cap Analog (TriLink BioTechnologies), and the template DNA ...
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bioRxiv - Microbiology 2022Quote: ... 500 μM 3’-dUTP (TriLink), or serially diluted s2UTP (0.625 to 10 mM)] were added and incubated at 30°C for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genes were targeted using two sgRNAs designed against regions at the 5’ end of each gene following the method outlined by Bassett et al.76 sgRNAs and Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) were injected into D ...
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.2 mM CleanCap AG (3′ OMe) (Trilink) was added to the IVT reaction by T7 RNAP or T7 RNAP-G47W to obtain 5′-capped GFP RNA ...
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bioRxiv - Biochemistry 2020Quote: ... and 2’-Azido-2’-dATP (TriLink BioTechnologies, San Diego, California) in 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Genetics 2019Quote: ... Nucleic acid dosages were as follows: 1.5 μg Cas9 mRNA (Trilink Biotechnologies), 1 μg each chemically modified sgRNA (Synthego) ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 mM m1Ψ (TriLink, Cat#N-1019-1). The IVT reaction can be scaled down by a half ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Physiology 2021Quote: ... and CleanCap® Reagent AG (6 mM, N-7113, TriLink Biotechnologies, USA) (Zangi et al. ...
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bioRxiv - Genomics 2021Quote: ... 0.5 mM 3′-azido-ddGTP (Trilink, #N-4008), 0.2 mM S-adenosylmethionine (SAM ...
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bioRxiv - Biophysics 2021Quote: ... Germany) and 3’-dATP was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dUTP (ref N-3005) was purchased from Trilink. Quant-it Picogreen® dsDNA assay kit (ref P11496 ...
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bioRxiv - Immunology 2023Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog (3′- OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG (Biolabmix) ...
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bioRxiv - Immunology 2024Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG ...
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bioRxiv - Cancer Biology 2024Quote: ... Modified aptamers that carried both 2’FY and 2’OMeR were purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10×Buffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG) ...
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bioRxiv - Immunology 2019Quote: ... Mouse C57BL/6 zygotes (Taconic) were injected with a mix of Cas9 mRNA (Trilink Biotechnologies) (100 ng/uL ...
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bioRxiv - Cell Biology 2023Quote: ... and CpG 2 µg/ml (TriLink) were added in a final volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: ... The CleanCap Reagent AG (3′ OMe) (TriLink; cat. no. N-7413) was added to the in vitro transcription reaction mixture for co-transcriptional 5′-end capping ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was further 3’-end-labeled with azide-dUTP (TriLink biotechnologies) using yeast poly(A ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μM dPTP (TriLink BioTechnologies; N-2037), and 2.5 U Taq polymerase in Thermopol Reaction Buffer (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: 2’O-methyl SSOs were provided by Trilink BioTechnologies ...
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bioRxiv - Biochemistry 2020Quote: ... and 2’-Azido-UTP were purchased from Trilink BioTechnologies (San Diego ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...