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Citations for TriLink BioTechnologies :
101 - 150 of 167 citations for 6H Benzo g 1 4 diazonino 7 6 5 cd indol 6 one 10 ethenyl 1 3 4 5 7 8 10 11 12 13 decahydro 4 hydroxymethyl 8 10 13 trimethyl 7 13 bis 1 methylethyl 4S 4R* 7R* 10S* 13S* 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Immunology 2022Quote: ... with 2.5 mM N1-Methylpseudouridine-5’-triphosphate nucleoside analog (TriLink BioTechnologies) instead of unmodified UTP ...
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Bioengineering 2022Quote: CleanCap Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) was purchased from TriLink Biotechnologies ...
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bioRxiv - Molecular Biology 2019Quote: ... ATP was replaced by N6-Methyladenosine-5’-Triphosphate(m6ATP) (Trilink-N-1013;) for the IVT reaction of m6A-modified RNA ...
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bioRxiv - Biochemistry 2021Quote: An RNA 16mer was chemically synthesized with either 5’ Cap (TriLink BioTechnologies) or 5’ Triphosphate (Chemgenes) ...
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bioRxiv - Molecular Biology 2022Quote: ... UTP was replaced with either pseudouridine-5’-triphosphate (N-1019, TriLink Biotechnologies) or N1-Methylpseudouridine-5’-Triphosphate (N-1081 ...
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bioRxiv - Biochemistry 2023Quote: ... and full replacement of UTP with N1-Methylpseudouridine-5’-triphosphate (TriLink Biotechnologies). After 1 hour of in vitro transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction was set up using ARCA (NEB, S1411L or Trilink, N-7003-5) and N1-Methylpseudouridine-5’-Triphosphate (Trilink ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: The 5’-triphosphorylated 29-nt RNAs used for capping reactions were synthesized by Trilink. The 22- ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Biophysics 2019Quote: ... transcription was chased by adding 40 µM NTPs mix together with 50 µM of each type of 3’-deoxynucleotide RNA chain terminators (3’dATP, 3’dCTP, 3’dGTP, 3’dUTP, TriLink Biotechnologies). The reactions were allowed to proceed at room temperature for 10 min before terminated by adding the 2× urea stop buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 mM 5-methylcytidine triphosphate and 7.5 mM pseudo-uridine triphosphate (TriLink Biotechnologies, San Diego, CA). Transfections of modRNA and multiple siRNA targeted against Sirt1 and Satb2 (both from Santa Cruz ...
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bioRxiv - Systems Biology 2023Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Genomics 2024Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Cell Biology 2021Quote: ... and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7701).
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bioRxiv - Immunology 2020Quote: ... Uridine 5’-triphosphate (UTP) was replaced with the triphosphate derivative of m1Ψ (m1ΨTP) (TriLink, San Diego, CA, USA Biotechnologies) in the transcription reaction ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at ∼70% confluency and reverse transfected with indicated concentrations of mRNA-Cas9-HA containing 5-methoxyuridine (5moU) or mRNA-eGFP 5moU (TriLink) in MessengerMAX (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Unmodified synthesized ANKRD55 mRNA transcript was capped at the 5’ end using wild-type bases CleanCap® AG (TriLink BioTechnologies) to generate a natural Cap 1 structure ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP, TriLink Biotechnologies), 1 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: There were two sources for the mRNA used in this study: 1) commercial synthesis by TriLink Biotechnologies (TriLink mRNA was used in Fig ...
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bioRxiv - Immunology 2023Quote: ... for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies, # N-7113-1). For large-scale mRNA production ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Immunology 2021Quote: ... 5′ amino-oligonucleotides were conjugated directly to each antigen using the SoluLINK Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... synthesis of mRNA was carried out using MEGAscript™ T7 Transcription Kit (Waltham, MA, USA) with the addition of ACRA 5’ cap (Trilink biotechnologies) and m1Ψ-5’-triphosphate (Trilink biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... 500 μM 3’-dUTP (TriLink), or serially diluted s2UTP (0.625 to 10 mM)] were added and incubated at 30°C for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genes were targeted using two sgRNAs designed against regions at the 5’ end of each gene following the method outlined by Bassett et al.76 sgRNAs and Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) were injected into D ...
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bioRxiv - Molecular Biology 2023Quote: ... with complete substitution of uridine to 1- methylpseudouridine and CleanCap AG analog (N-1081 and N-7113, TriLink Biotechnologies).
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bioRxiv - Synthetic Biology 2024Quote: ... transduced activation reporter cells were enriched by puromycin selection (1µg mL-1) following nucleofection of dCas9-VPR mRNA (TriLink) and a Cas9 sgRNA targeting the ESR protospacer (IDT) ...
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bioRxiv - Cancer Biology 2021Quote: ... Two sgRNAs against SCP4 and one sgRNA against MYC were synthesized by TriLink BioTechnologies ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the single-site RosaBxb-GT host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired attP-GT carrier strains ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the dual-site RosaBxb-GT/GA host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired strain ...
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bioRxiv - Biophysics 2022Quote: ... methodology described by Samnuan (11) and the capping using the CleanCap ® Reagent AG performed as per the manufactures protocol (Trilink Biotechnologies, USA). After the IVT reaction was complete the DNA template was digested using DNase 1 enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.2 mM CleanCap AG (3′ OMe) (Trilink) was added to the IVT reaction by T7 RNAP or T7 RNAP-G47W to obtain 5′-capped GFP RNA ...
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bioRxiv - Genomics 2021Quote: ... 0.5 mM 3′-azido-ddGTP (Trilink, #N-4008), 0.2 mM S-adenosylmethionine (SAM ...
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bioRxiv - Biophysics 2021Quote: ... Germany) and 3’-dATP was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) and placed in the 20µl cuvette provided and CB-150 was the Amaxa protocol used ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were electroporated with 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) codon optimized ABE7.10 mRNA (TriLink Biotechnologies ...