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Citations for TriLink BioTechnologies :
1 - 50 of 62 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... or 7-deaza-ATP (TriLink, N-1061-1) during in vitro transcription using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 7-deaza-GTP (Trilink Biotechnologies, USA) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... and either GTP or 7-deaza-GTP 10mM each (7-Deazaguanosine-5’-Triphosphate, TriLink, San Diego, CA, USA). One-cell staged embryos were microinjected with 200 and 400 pg of the transcripts and incubated at 28°C until they reached the stage for collection indicated for each experiment ...
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bioRxiv - Genomics 2022Quote: ... 19uL of the solution was combined with 1.5uL 10mM dATP-NH2 (7-Deaza-7-Propargylamino-2’-deoxyadenosine-5’-Triphosphate from Trilink N-2068), 8.0uL 3.75mM 2kD Biotin-PEG-NH2 (Laysan Bio Item# Biotin-PEG-NH2-2K-1g ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) and synthesized in vitro as described in RNA preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP or ATP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) or 7-deaza-ATP (TriLink ...
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bioRxiv - Cancer Biology 2024Quote: ... and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044) plus 0.75 mM GTP was used ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 6-(2-deoxy-beta-D-ribofuranosyl)-3,4-dihydro-8H-pyrimido-[4,5-C] [1,2]oxazin-7-one triphosphate (dPTP, Trilink Biotechnologies), 200nM each of forward and reverse primers ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Bioengineering 2019Quote: ... 8-oxo-dGT (TriLink, N-2034), each at 400 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 8-oxo-2’-deoxyguanosine-5’-triphosphate (8-oxo-dGTP) and 2’-deoxy-P-nucleoside-5’-triphosphate (dPTP) (TriLink). For each library ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we used the following reaction mixture: 150nM each of the nucleotide analogs 8-oxodeoxyguanosine triphosphate (8-oxo-dGTP, Trilink Biotechnologies) and 6-(2-deoxy-beta-D-ribofuranosyl)-3,4-dihydro-8H-pyrimido-[4,5-C] [1,2]oxazin-7-one triphosphate (dPTP ...
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bioRxiv - Bioengineering 2020Quote: ... 1 ng was used as template for initial error-prone PCR using 8-oxo-GTP and dPTP (TriLink Biotechnologies). Four 50 µl PCR reactions were performed on each template with increasing concentrations of mutagenic nucleotides (5 µM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 3 μM of 8-oxo-GTP/dPTP (Trilink Biotechnologies), and 400 nM of each primer (MutafpF-GAAGGAGATATACctcgag /MutafpR- AGACCGTTTAAACaagctt) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μM 8-oxo-dGTP (TriLink BioTechnologies, N-2034), 2 μM dPTP (TriLink BioTechnologies ...
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bioRxiv - Cancer Biology 2019Quote: ... and nucleotide analogs 8-oxo-dGTP and dPTP (TriLink Biotech) as described previously37,38 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 mM Anti Reverse Cap Analog (TriLink BioTechnologies), and the template DNA ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... and the nucleotide analogs 8-oxo-dGTP and dPTP (TriLink Biotech). Six separate PCR reactions were performed in which the concentration of analogs and the number of cycles were varied to obtain a range of mutation frequencies ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Physiology 2021Quote: ... and CleanCap® Reagent AG (6 mM, N-7113, TriLink Biotechnologies, USA) (Zangi et al. ...
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bioRxiv - Genomics 2022Quote: ... 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated 4-thiouridine (TriLink, N-1025). Different strands of pre-mRNA were generated for each construct that included different quantities of 4sU ...
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bioRxiv - Immunology 2019Quote: ... Mouse C57BL/6 zygotes (Taconic) were injected with a mix of Cas9 mRNA (Trilink Biotechnologies) (100 ng/uL ...
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bioRxiv - Evolutionary Biology 2023Quote: epPCR libraries - PTE libraries were generated by random mutagenesis with error-prone PCR (epPCR) using the ‘wobble’ base analogues dPTP and 8-oxo-dGTP (TriLink), with slight alterations from reference 16 ...
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bioRxiv - Immunology 2021Quote: Short mitochondrial DNA fragments (221bp) were amplified from gDNA from A549 cells with and without 8-Oxo-2’-deoxyguanosine-5’-Triphosphate (TriLink Biotechnologies) using GoTaq Mastermix ...
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bioRxiv - Biochemistry 2022Quote: ... by adding 2.5 μL each of 20 μM 8-oxo-dGTP TriLink Biotechnologies N-2034) and 20 μM dPTP (TriLink Biotechnologies N-2037) to a 50 μL Taq polymerase (Fisher Scientific 50-811-694 ...
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bioRxiv - Bioengineering 2019Quote: ... 6 μg of Cas9 (Integrated DNA Technologies, IA, Cat: 1074182) and 3.2 μg of MS-sgRNA (Trilink Biotechnologies, CA) (molar ratio = 1:2.5 ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Synthetic Biology 2023Quote: ... along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink, N-7113). 5 mM N1-Methylpseudouridine-5’-triphosphate (Trilink ...
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bioRxiv - Bioengineering 2023Quote: ... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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bioRxiv - Bioengineering 2023Quote: ... was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA that was either purchased by TriLink (most experiments) or made in-house via in vitro transcription (IVT)40 at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Immunology 2023Quote: ... with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies, #N-1081-1) for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2022Quote: ... 1-methylpseudouridine-5′-triphosphate (TriLink) was used instead of UTP to generate modified nucleoside-containing mRNA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Microbiology 2020Quote: ... 2’-F-2’-dUTP (N-1010-1) and didanosine-TP (N-4017-1) were purchased from Trilink. Remdesivir-DP was synthesized by WuXi AppTech ...
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Genomics 2022Quote: ... 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 mM m1Ψ (TriLink, Cat#N-1019-1). The IVT reaction can be scaled down by a half ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Genomics 2023Quote: ... using 5-Methyluridine-5’-Triphosphate (5-mUTP, Trilink, N-1024-1) instead of UTP ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 5’-O-(1-thiotriphosphate) and 3’-deoxycytidine 5’-triphosphate were from TriLink. Adenosine 5’-O-(1-thiotriphosphate ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP, TriLink Biotechnologies), 1 mM ATP ...
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bioRxiv - Bioengineering 2023Quote: There were two sources for the mRNA used in this study: 1) commercial synthesis by TriLink Biotechnologies (TriLink mRNA was used in Fig ...
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bioRxiv - Immunology 2023Quote: ... for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies, # N-7113-1). For large-scale mRNA production ...