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Citations for TriLink BioTechnologies :
51 - 100 of 120 citations for 6 METHYL 7 1 METHYL 2 OXO PROPOXY 2 3 DIHYDRO 1H CYCLOPENTA C CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044) plus 0.75 mM GTP was used ...
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bioRxiv - Microbiology 2022Quote: ... 500 μM 3’-dUTP (TriLink), or serially diluted s2UTP (0.625 to 10 mM)] were added and incubated at 30°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... which was used to attach Cy3B/BHQ-2 labeled DNA oligonucleotides via RNA/DNA hybridization (labeled DNA oligonucleotides purchased from TriLink Technologies). Individual tRNA species used were purchased from Chemical Block Ltd ...
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bioRxiv - Molecular Biology 2022Quote: ... a fixed total RNA volume of 2 μl was used for library preparation using the CleanTag kit (TriLink, San Diego, USA). Adapter dilution (1:4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal volumes of total RNA (2 μL) were used for small RNA library preparation using the Clean Tag small RNA library preparation kit (TriLink Biotechnologies, US). that utilizes chemically modified adapters to prevent formation of adapter dimers 53 ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Cancer Biology 2021Quote: ... Two sgRNAs against SCP4 and one sgRNA against MYC were synthesized by TriLink BioTechnologies ...
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bioRxiv - Microbiology 2021Quote: ... UTP was replaced with One-methylpseudouridine (m1Ψ)-5’-triphosphate (TriLink, Cat# N-1081) for producing nucleoside-modified mRNAs ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.2 mM CleanCap AG (3′ OMe) (Trilink) was added to the IVT reaction by T7 RNAP or T7 RNAP-G47W to obtain 5′-capped GFP RNA ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Physiology 2021Quote: ... and CleanCap® Reagent AG (6 mM, N-7113, TriLink Biotechnologies, USA) (Zangi et al. ...
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bioRxiv - Genomics 2021Quote: ... 0.5 mM 3′-azido-ddGTP (Trilink, #N-4008), 0.2 mM S-adenosylmethionine (SAM ...
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bioRxiv - Biophysics 2021Quote: ... Germany) and 3’-dATP was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Genomics 2022Quote: ... 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated 4-thiouridine (TriLink, N-1025). Different strands of pre-mRNA were generated for each construct that included different quantities of 4sU ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dUTP (ref N-3005) was purchased from Trilink. Quant-it Picogreen® dsDNA assay kit (ref P11496 ...
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bioRxiv - Immunology 2024Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG ...
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bioRxiv - Immunology 2023Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog (3′- OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG (Biolabmix) ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μM capped 16-nucleotide RNAs (5’-m7GpppGAAUGCUAUAAUAGC-3’, Trilink), 2 U μL-1 RNasin (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 mM ApA RNA dinucleotide (5’ to 3’; TriLink BioTechnologies) was suspended in degassed 20 mM HEPES/NaOH pH 7.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10×Buffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG) ...
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bioRxiv - Immunology 2019Quote: ... Mouse C57BL/6 zygotes (Taconic) were injected with a mix of Cas9 mRNA (Trilink Biotechnologies) (100 ng/uL ...
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bioRxiv - Biochemistry 2021Quote: ... 3′-amino (NH2) dTTP and dideoxy TTP (ddTTP) were from TriLink Bio Technologies ...
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bioRxiv - Immunology 2023Quote: ... The CleanCap Reagent AG (3′ OMe) (TriLink; cat. no. N-7413) was added to the in vitro transcription reaction mixture for co-transcriptional 5′-end capping ...
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bioRxiv - Bioengineering 2023Quote: dAbs were generated by conjugating amine-modified oligonucleotides (15–25 OD260 units) to polyclonal antibodies (100 µg) using the Antibody-Oligonucleotide All-in-One Conjugation Kit from TriLink Biotechnologies (A-9202) ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was further 3’-end-labeled with azide-dUTP (TriLink biotechnologies) using yeast poly(A ...
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bioRxiv - Bioengineering 2019Quote: ... 6 μg of Cas9 (Integrated DNA Technologies, IA, Cat: 1074182) and 3.2 μg of MS-sgRNA (Trilink Biotechnologies, CA) (molar ratio = 1:2.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... and Buffer C is the buffer recommended by the CleanCap® AG manufacturer (Trilink) for use with WT T7RNAP ...
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bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction included CleanCap®Reagent AG (3’ OMe) (Trilink, CA, USA) for co-transcriptional capping of mRNA and complete replacement of uridine by N1-methyl-pseudouridine (Trilink ...
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bioRxiv - Microbiology 2021Quote: ... Capped 14-mer ending by “AG” (5’-m7Gppp GAA UGC UAU AAU AG-3’) (TriLink Biotechnologies) and in vitro produced capped 14-mer ending by “AGU” (5’-m7Gppp GGA UGC UAU AUA GU-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... dRT (1.5-3 ng/μl) and Cas9 mRNA (100 ng/μl, Trilink Biotechnologies, San Diego, CA) was microinjected into pronuclei and cytoplasm of one-cell stage embryos obtained from superovulated B6D2F1 female mice (Jackson Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: ... we used T7 RNA polymerase in the presence of a CleanCap Reagent AG (3’ OMe) (TriLink) on linearized plasmids encoding codon-optimized SARS-CoV-2 gene ...
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bioRxiv - Immunology 2022Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’- TCGTCGTTGTCGTTTTGTCGTT-3’) (TriLink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Immunology 2023Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’-TCGTCGTTGTCGTTTTGTCGTT-3’) (Trilink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Synthetic Biology 2023Quote: ... along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink, N-7113). 5 mM N1-Methylpseudouridine-5’-triphosphate (Trilink ...
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bioRxiv - Immunology 2019Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Cancer Biology 2021Quote: ... KYAT1 mRNA with pseudouridine incorporating a 3XmiR122 antisense site in the same relative locations within the 3’ UTR as described by Ruchi et al (18) was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Immunology 2021Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Immunology 2022Quote: ... of c-srRNA with Cap1 and polyA was performed using in vitro transcription of a plasmid DNA with Cleancap AU (Trilink) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... RNA sequencing ladders were generated by walking elongation complexes to +15 before adding NTPs supplemented with a chain terminating 3’-deoxyNTP (TriLink Biotechnologies) to 100 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... The lipid mixture was obtained with 25 mM sodium acetate buffer (pH 3; T&I, Gangwon-do, Korea) containing Renilla luciferase mRNA (TriLink, CA, USA) at a N/P ratio of 5.6 ...
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bioRxiv - Bioengineering 2023Quote: ... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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bioRxiv - Bioengineering 2023Quote: ... was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA that was either purchased by TriLink (most experiments) or made in-house via in vitro transcription (IVT)40 at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Bioengineering 2022Quote: ... 1-methylpseudouridine-5′-triphosphate (TriLink) was used instead of UTP to generate modified nucleoside-containing mRNA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...