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Citations for TriLink BioTechnologies :
51 - 91 of 91 citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... we used T7 RNA polymerase in the presence of a CleanCap Reagent AG (3’ OMe) (TriLink) on linearized plasmids encoding codon-optimized SARS-CoV-2 gene ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’- TCGTCGTTGTCGTTTTGTCGTT-3’) (TriLink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Immunology 2023Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’-TCGTCGTTGTCGTTTTGTCGTT-3’) (Trilink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Immunology 2019Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Cancer Biology 2021Quote: ... KYAT1 mRNA with pseudouridine incorporating a 3XmiR122 antisense site in the same relative locations within the 3’ UTR as described by Ruchi et al (18) was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Immunology 2021Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Biochemistry 2020Quote: ... RNA sequencing ladders were generated by walking elongation complexes to +15 before adding NTPs supplemented with a chain terminating 3’-deoxyNTP (TriLink Biotechnologies) to 100 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... The lipid mixture was obtained with 25 mM sodium acetate buffer (pH 3; T&I, Gangwon-do, Korea) containing Renilla luciferase mRNA (TriLink, CA, USA) at a N/P ratio of 5.6 ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Immunology 2023Quote: ... with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies, #N-1081-1) for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2022Quote: ... 1-methylpseudouridine-5′-triphosphate (TriLink) was used instead of UTP to generate modified nucleoside-containing mRNA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Genomics 2022Quote: ... 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink ...
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bioRxiv - Molecular Biology 2021Quote: ... or 7-deaza-ATP (TriLink, N-1061-1) during in vitro transcription using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 mM m1Ψ (TriLink, Cat#N-1019-1). The IVT reaction can be scaled down by a half ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Genomics 2023Quote: ... using 5-Methyluridine-5’-Triphosphate (5-mUTP, Trilink, N-1024-1) instead of UTP ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) and synthesized in vitro as described in RNA preparation ...
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bioRxiv - Bioengineering 2023Quote: There were two sources for the mRNA used in this study: 1) commercial synthesis by TriLink Biotechnologies (TriLink mRNA was used in Fig ...
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bioRxiv - Immunology 2023Quote: ... for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies, # N-7113-1). For large-scale mRNA production ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP or ATP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) or 7-deaza-ATP (TriLink ...
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bioRxiv - Systems Biology 2019Quote: eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2020Quote: ... 1 ng was used as template for initial error-prone PCR using 8-oxo-GTP and dPTP (TriLink Biotechnologies). Four 50 µl PCR reactions were performed on each template with increasing concentrations of mutagenic nucleotides (5 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... with complete substitution of uridine to 1- methylpseudouridine and CleanCap AG analog (N-1081 and N-7113, TriLink Biotechnologies).
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bioRxiv - Synthetic Biology 2024Quote: ... transduced activation reporter cells were enriched by puromycin selection (1µg mL-1) following nucleofection of dCas9-VPR mRNA (TriLink) and a Cas9 sgRNA targeting the ESR protospacer (IDT) ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the single-site RosaBxb-GT host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired attP-GT carrier strains ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the dual-site RosaBxb-GT/GA host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired strain ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 104 cells were harvested and incubated in 10 ml Neon Buffer R with 1 ul (1ug/ul) of Clean-Cap Cas9 mRNA (TriLink Biotechnologies) and 1 ul (100 pmol/ul ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) and placed in the 20µl cuvette provided and CB-150 was the Amaxa protocol used ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were electroporated with 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) codon optimized ABE7.10 mRNA (TriLink Biotechnologies ...
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bioRxiv - Immunology 2023Quote: ... was prepared in injection buffer by mixing 500 ng/μl of each of the sgRNAs (left and right) with Cas9 mRNA (1 μg/μl, TriLink, cat # L-6125). Fertilized eggs collected from B6/129 mice were microinjected at the CHOP transgenic core and transferred into pseudo-pregnant B6 females ...