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Citations for TriLink BioTechnologies :
51 - 93 of 93 citations for 5 tert butoxy 5 oxopentanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and of N1-methylpseudouridine-5’-triphosphate (Cat: N-1081, TriLink) in place of uridine-5’-triphosphate (UTP) ...
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bioRxiv - Immunology 2022Quote: ... Nucleoside-5’-Triphosphate (NTP) Set (TriLink cat. no. N-1505), T7 RNA polymerase (New England BioLabs cat ...
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bioRxiv - Immunology 2022Quote: ... with 2.5 mM N1-Methylpseudouridine-5’-triphosphate nucleoside analog (TriLink BioTechnologies) instead of unmodified UTP ...
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bioRxiv - Bioengineering 2022Quote: CleanCap Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) was purchased from TriLink Biotechnologies ...
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bioRxiv - Molecular Biology 2019Quote: ... ATP was replaced by N6-Methyladenosine-5’-Triphosphate(m6ATP) (Trilink-N-1013;) for the IVT reaction of m6A-modified RNA ...
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bioRxiv - Biochemistry 2021Quote: An RNA 16mer was chemically synthesized with either 5’ Cap (TriLink BioTechnologies) or 5’ Triphosphate (Chemgenes) ...
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bioRxiv - Molecular Biology 2022Quote: ... UTP was replaced with either pseudouridine-5’-triphosphate (N-1019, TriLink Biotechnologies) or N1-Methylpseudouridine-5’-Triphosphate (N-1081 ...
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bioRxiv - Biochemistry 2023Quote: ... and full replacement of UTP with N1-Methylpseudouridine-5’-triphosphate (TriLink Biotechnologies). After 1 hour of in vitro transcription ...
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bioRxiv - Microbiology 2021Quote: ... UTP was replaced with One-methylpseudouridine (m1Ψ)-5’-triphosphate (TriLink, Cat# N-1081) for producing nucleoside-modified mRNAs ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction was set up using ARCA (NEB, S1411L or Trilink, N-7003-5) and N1-Methylpseudouridine-5’-Triphosphate (Trilink ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: The 5’-triphosphorylated 29-nt RNAs used for capping reactions were synthesized by Trilink. The 22- ...
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bioRxiv - Immunology 2023Quote: ... with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies, #N-1081-1) for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Microbiology 2021Quote: ... Capped 14-mer ending by “AG” (5’-m7Gppp GAA UGC UAU AAU AG-3’) (TriLink Biotechnologies) and in vitro produced capped 14-mer ending by “AGU” (5’-m7Gppp GGA UGC UAU AUA GU-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 mM 5-methylcytidine triphosphate and 7.5 mM pseudo-uridine triphosphate (TriLink Biotechnologies, San Diego, CA). Transfections of modRNA and multiple siRNA targeted against Sirt1 and Satb2 (both from Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription in the presence of a mixture of 5’-biotin-G-monophosphate (TriLink Biotechnologies) and GTP (molar ratio 3:2 ...
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bioRxiv - Systems Biology 2023Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Genomics 2024Quote: ... and co-transcriptional 5’ capping with the CleanCap AG analog (Trilink BioTechnologies Cat No. N-7113) to generate a 5’ Cap1 structure ...
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bioRxiv - Immunology 2022Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’- TCGTCGTTGTCGTTTTGTCGTT-3’) (TriLink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Immunology 2023Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’-TCGTCGTTGTCGTTTTGTCGTT-3’) (Trilink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Bioengineering 2020Quote: ... All mRNAs were synthesized by in vitro transcription with 100% substitution of UTP by pseudouridine-5’-triphosphate (TriLink) using AmpliScribe T7-Flash Transcription Kit (Lucigen ...
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bioRxiv - Cell Biology 2021Quote: ... and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7701).
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bioRxiv - Molecular Biology 2023Quote: ... and either GTP or 7-deaza-GTP 10mM each (7-Deazaguanosine-5’-Triphosphate, TriLink, San Diego, CA, USA). One-cell staged embryos were microinjected with 200 and 400 pg of the transcripts and incubated at 28°C until they reached the stage for collection indicated for each experiment ...
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bioRxiv - Molecular Biology 2022Quote: ... the 3’-end azide functionalization in presence of 2’-azido-2’-deoxyadenosine-5’triphosphate (ATP-azide, Trilink Biotechnologies) using yeast poly(A ...
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bioRxiv - Immunology 2020Quote: ... Uridine 5’-triphosphate (UTP) was replaced with the triphosphate derivative of m1Ψ (m1ΨTP) (TriLink, San Diego, CA, USA Biotechnologies) in the transcription reaction ...
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Evaluation of mRNA-LNP and adjuvanted protein SARS-CoV-2 vaccines in a maternal antibody mouse modelbioRxiv - Immunology 2023Quote: ... diproline-modified spike protein from the SARS-CoV-2 Wuhan-Hu-1 strain were produced as previously described15,16 with m1Ψ-5-triphosphate (TriLink) instead of UTP and capped cotranscriptionally using the trinucleotide cap1 analog ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at ∼70% confluency and reverse transfected with indicated concentrations of mRNA-Cas9-HA containing 5-methoxyuridine (5moU) or mRNA-eGFP 5moU (TriLink) in MessengerMAX (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Unmodified synthesized ANKRD55 mRNA transcript was capped at the 5’ end using wild-type bases CleanCap® AG (TriLink BioTechnologies) to generate a natural Cap 1 structure ...
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bioRxiv - Immunology 2021Quote: ... 5′ amino-oligonucleotides were conjugated directly to each antigen using the SoluLINK Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Short mitochondrial DNA fragments (221bp) were amplified from gDNA from A549 cells with and without 8-Oxo-2’-deoxyguanosine-5’-Triphosphate (TriLink Biotechnologies) using GoTaq Mastermix ...
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bioRxiv - Immunology 2020Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 19uL of the solution was combined with 1.5uL 10mM dATP-NH2 (7-Deaza-7-Propargylamino-2’-deoxyadenosine-5’-Triphosphate from Trilink N-2068), 8.0uL 3.75mM 2kD Biotin-PEG-NH2 (Laysan Bio Item# Biotin-PEG-NH2-2K-1g ...
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bioRxiv - Molecular Biology 2022Quote: ... 21-mer-sd or 21-mer-d 3′ and 14-mer 5′ ends vRNAs (IBA) were used in combination with synthetic 12-or 13-mer capped RNA (TriLink Biotechnologies) as primer (Table 2 ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Bioengineering 2023Quote: ... synthesis of mRNA was carried out using MEGAscript™ T7 Transcription Kit (Waltham, MA, USA) with the addition of ACRA 5’ cap (Trilink biotechnologies) and m1Ψ-5’-triphosphate (Trilink biotechnologies ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genes were targeted using two sgRNAs designed against regions at the 5’ end of each gene following the method outlined by Bassett et al.76 sgRNAs and Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) were injected into D ...
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bioRxiv - Genetics 2019Quote: ... Nucleic acid dosages were as follows: 1.5 μg Cas9 mRNA (Trilink Biotechnologies), 1 μg each chemically modified sgRNA (Synthego) ...
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bioRxiv - Microbiology 2021Quote: ... pseudouridine-substituted Fc-conjugated RBD mRNA (amino acids 331– 524; GenBank: QHD43416.1) was purchased from TriLink Bio Technologies (San Diego ...