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Citations for TriLink BioTechnologies :
101 - 150 of 172 citations for 6 methyl 5 6 7 8 tetrahydro 1 3 dioxolo 4 5 g isoquinolin 6 iumchloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The NEB HiScribe High-Yield Kit was used as per the instruction manual but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (Trilink). Reaction cleanup was performed by lithium chloride precipitation ...
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bioRxiv - Microbiology 2020Quote: ... The standards Am and m6Am 5’ monophosphate were generated by digesting their triphosphate forms (TriLink N-1015 and N-1112) with 1 unit Apyrase (NEB M0398).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at ∼70% confluency and reverse transfected with indicated concentrations of mRNA-Cas9-HA containing 5-methoxyuridine (5moU) or mRNA-eGFP 5moU (TriLink) in MessengerMAX (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Unmodified synthesized ANKRD55 mRNA transcript was capped at the 5’ end using wild-type bases CleanCap® AG (TriLink BioTechnologies) to generate a natural Cap 1 structure ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μM 8-oxo-dGTP (TriLink BioTechnologies, N-2034), 2 μM dPTP (TriLink BioTechnologies ...
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bioRxiv - Genomics 2021Quote: ... was used according to the company’s recommended instruction but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (TriLink Biotechnologies). RNA was precipitated with lithium chloride (2.5 M final concentration) ...
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bioRxiv - Immunology 2021Quote: ... 5′ amino-oligonucleotides were conjugated directly to each antigen using the SoluLINK Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5’amino-oligonucleotides were conjugated directly to each antigen using the Solulink Protein-Oligonucleotide Conjugation Kit (TriLink cat no. S-9011) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 21-mer-sd or 21-mer-d 3′ and 14-mer 5′ ends vRNAs (IBA) were used in combination with synthetic 12-or 13-mer capped RNA (TriLink Biotechnologies) as primer (Table 2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and nucleotide analogs 8-oxo-dGTP and dPTP (TriLink Biotech) as described previously37,38 ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Bioengineering 2023Quote: ... synthesis of mRNA was carried out using MEGAscript™ T7 Transcription Kit (Waltham, MA, USA) with the addition of ACRA 5’ cap (Trilink biotechnologies) and m1Ψ-5’-triphosphate (Trilink biotechnologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044) plus 0.75 mM GTP was used ...
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bioRxiv - Microbiology 2022Quote: ... 500 μM 3’-dUTP (TriLink), or serially diluted s2UTP (0.625 to 10 mM)] were added and incubated at 30°C for 10 min ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... and the nucleotide analogs 8-oxo-dGTP and dPTP (TriLink Biotech). Six separate PCR reactions were performed in which the concentration of analogs and the number of cycles were varied to obtain a range of mutation frequencies ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Cell Biology 2021Quote: ... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genes were targeted using two sgRNAs designed against regions at the 5’ end of each gene following the method outlined by Bassett et al.76 sgRNAs and Cas9 mRNA (TriLink Biotechnologies, San Diego, CA) were injected into D ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.2 mM CleanCap AG (3′ OMe) (Trilink) was added to the IVT reaction by T7 RNAP or T7 RNAP-G47W to obtain 5′-capped GFP RNA ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Genomics 2021Quote: ... 0.5 mM 3′-azido-ddGTP (Trilink, #N-4008), 0.2 mM S-adenosylmethionine (SAM ...
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bioRxiv - Biophysics 2021Quote: ... Germany) and 3’-dATP was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 6-(2-deoxy-beta-D-ribofuranosyl)-3,4-dihydro-8H-pyrimido-[4,5-C] [1,2]oxazin-7-one triphosphate (dPTP, Trilink Biotechnologies), 200nM each of forward and reverse primers ...
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bioRxiv - Genomics 2022Quote: ... 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and phosphorylated 4-thiouridine (TriLink, N-1025). Different strands of pre-mRNA were generated for each construct that included different quantities of 4sU ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dUTP (ref N-3005) was purchased from Trilink. Quant-it Picogreen® dsDNA assay kit (ref P11496 ...
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bioRxiv - Immunology 2023Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog (3′- OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG (Biolabmix) ...
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bioRxiv - Immunology 2024Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10xBuffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl T7 RNA polymerase (Biolabmix) and 10×Buffer (TriLink), 4 mM trinucleotide cap 1 analog ((3′-OMe-m7G)-5′-ppp-5′-(2′-OMeA)pG) ...
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bioRxiv - Biochemistry 2021Quote: ... 3′-amino (NH2) dTTP and dideoxy TTP (ddTTP) were from TriLink Bio Technologies ...
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bioRxiv - Immunology 2023Quote: ... The CleanCap Reagent AG (3′ OMe) (TriLink; cat. no. N-7413) was added to the in vitro transcription reaction mixture for co-transcriptional 5′-end capping ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was further 3’-end-labeled with azide-dUTP (TriLink biotechnologies) using yeast poly(A ...
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bioRxiv - Evolutionary Biology 2023Quote: epPCR libraries - PTE libraries were generated by random mutagenesis with error-prone PCR (epPCR) using the ‘wobble’ base analogues dPTP and 8-oxo-dGTP (TriLink), with slight alterations from reference 16 ...
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bioRxiv - Biochemistry 2022Quote: ... by adding 2.5 μL each of 20 μM 8-oxo-dGTP TriLink Biotechnologies N-2034) and 20 μM dPTP (TriLink Biotechnologies N-2037) to a 50 μL Taq polymerase (Fisher Scientific 50-811-694 ...
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bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction included CleanCap®Reagent AG (3’ OMe) (Trilink, CA, USA) for co-transcriptional capping of mRNA and complete replacement of uridine by N1-methyl-pseudouridine (Trilink ...
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bioRxiv - Molecular Biology 2022Quote: ... dRT (1.5-3 ng/μl) and Cas9 mRNA (100 ng/μl, Trilink Biotechnologies, San Diego, CA) was microinjected into pronuclei and cytoplasm of one-cell stage embryos obtained from superovulated B6D2F1 female mice (Jackson Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: ... we used T7 RNA polymerase in the presence of a CleanCap Reagent AG (3’ OMe) (TriLink) on linearized plasmids encoding codon-optimized SARS-CoV-2 gene ...
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bioRxiv - Synthetic Biology 2023Quote: ... along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink, N-7113). 5 mM N1-Methylpseudouridine-5’-triphosphate (Trilink ...
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bioRxiv - Immunology 2019Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Cancer Biology 2021Quote: ... KYAT1 mRNA with pseudouridine incorporating a 3XmiR122 antisense site in the same relative locations within the 3’ UTR as described by Ruchi et al (18) was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Immunology 2021Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Biochemistry 2020Quote: ... RNA sequencing ladders were generated by walking elongation complexes to +15 before adding NTPs supplemented with a chain terminating 3’-deoxyNTP (TriLink Biotechnologies) to 100 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... The lipid mixture was obtained with 25 mM sodium acetate buffer (pH 3; T&I, Gangwon-do, Korea) containing Renilla luciferase mRNA (TriLink, CA, USA) at a N/P ratio of 5.6 ...
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bioRxiv - Bioengineering 2023Quote: ... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
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bioRxiv - Bioengineering 2023Quote: ... was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA that was either purchased by TriLink (most experiments) or made in-house via in vitro transcription (IVT)40 at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Microbiology 2020Quote: ... 2’-F-2’-dUTP (N-1010-1) and didanosine-TP (N-4017-1) were purchased from Trilink. Remdesivir-DP was synthesized by WuXi AppTech ...