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Citations for TriLink BioTechnologies :
51 - 99 of 99 citations for 6 Hydroxy 2 oxo 2H 1 benzopyran 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... we used T7 RNA polymerase in the presence of a CleanCap Reagent AG (3’ OMe) (TriLink) on linearized plasmids encoding codon-optimized SARS-CoV-2 gene ...
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bioRxiv - Biophysics 2020Quote: ... except each nucleotide was present at a final concentration of 4 mM and the reaction was supplemented with 6 mM of 5’-biotin-G-Monophosphate (Trilink, #N-6003). Reactions were incubated at 37 °C for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
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bioRxiv - Immunology 2022Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’- TCGTCGTTGTCGTTTTGTCGTT-3’) (TriLink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Immunology 2023Quote: ... Animals were mock-immunized with the CpG oligonucleotide (5’-TCGTCGTTGTCGTTTTGTCGTT-3’) (Trilink Inc., Santa Fe Springs, CA) using 100 μg CpG/guinea pig and 150μg alum (Alhydrogel ...
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bioRxiv - Neuroscience 2021Quote: ... which were then plated at 500,000 cells per well in a Matrigel-coated 6-well plate for transfection with 1.2 ug of Cre recombinase mRNA (TriLink Biotechnologies cat. no. L-7211) using Lipofectamine Stem to remove the puromycin resistance gene and mCherry ...
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bioRxiv - Immunology 2019Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Cancer Biology 2021Quote: ... KYAT1 mRNA with pseudouridine incorporating a 3XmiR122 antisense site in the same relative locations within the 3’ UTR as described by Ruchi et al (18) was purchased from TriLink Biotechnologies (San Diego ...
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bioRxiv - Immunology 2021Quote: ... ODN 5’ TCG TCG TCG TTC GAA CGA CGT TGA T 3’ as the sodium salt on a phosphorothioate backbone (ODN M362) was purchased from TriLink BioTechnologies (San Diego ...
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bioRxiv - Biochemistry 2020Quote: ... RNA sequencing ladders were generated by walking elongation complexes to +15 before adding NTPs supplemented with a chain terminating 3’-deoxyNTP (TriLink Biotechnologies) to 100 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... The lipid mixture was obtained with 25 mM sodium acetate buffer (pH 3; T&I, Gangwon-do, Korea) containing Renilla luciferase mRNA (TriLink, CA, USA) at a N/P ratio of 5.6 ...
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bioRxiv - Bioengineering 2024Quote: ... a 1:1 mixture of CRISPR-Cas9 mRNA (TriLink, San Diego, CA, USA) to sgRNA (IDT ...
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bioRxiv - Immunology 2023Quote: ... with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies, #N-1081-1) for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Bioengineering 2022Quote: ... however 2 μl of the appropriate canonical NTP (75 mM) was replaced by 2 μl of 100 mM 5-Hydroxymethyl-5’-triphosphate (e.g. 5-Hydroxymethyl-CTP) (TriLink).
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bioRxiv - Cancer Biology 2023Quote: The long strand of the AsiC synthesized with 2′-fluoropyrimidines was annealed to the short antisense strand (TriLink Biotechnologies) with a 2-fold molar excess of the short strand ...
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bioRxiv - Bioengineering 2022Quote: ... 1-methylpseudouridine-5′-triphosphate (TriLink) was used instead of UTP to generate modified nucleoside-containing mRNA ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM XTP (TriLink Biotechnologies), or no nucleotide to acquire baseline measurement for 30 seconds ...
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bioRxiv - Molecular Biology 2019Quote: ... which was used to attach Cy3B/BHQ-2 labeled DNA oligonucleotides via RNA/DNA hybridization (labeled DNA oligonucleotides purchased from TriLink Technologies). Individual tRNA species used were purchased from Chemical Block Ltd ...
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bioRxiv - Genomics 2022Quote: ... 19uL of the solution was combined with 1.5uL 10mM dATP-NH2 (7-Deaza-7-Propargylamino-2’-deoxyadenosine-5’-Triphosphate from Trilink N-2068), 8.0uL 3.75mM 2kD Biotin-PEG-NH2 (Laysan Bio Item# Biotin-PEG-NH2-2K-1g ...
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bioRxiv - Molecular Biology 2022Quote: ... a fixed total RNA volume of 2 μl was used for library preparation using the CleanTag kit (TriLink, San Diego, USA). Adapter dilution (1:4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal volumes of total RNA (2 μL) were used for small RNA library preparation using the Clean Tag small RNA library preparation kit (TriLink Biotechnologies, US). that utilizes chemically modified adapters to prevent formation of adapter dimers 53 ...
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bioRxiv - Bioengineering 2022Quote: ... IVT reactions performed as described for unmodified with 2 μl of the appropriate unmodified 75 mM NTP replaced by 5 μl of 10 mM Sp α-thiophosphate NTP (Biolog) or 2 μl 100 mM Rp/Sp α-thiophosphate NTP (TriLink).
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bioRxiv - Biochemistry 2022Quote: ... λ271 nm = 7,800 L mol-1cm-1) or N1-propylpseudouridine triphosphate (p1ΨTP: λ271 nm = 8,900 L mol-1cm-1; Trilink Biotechnologies). All experiments were conducted in duplicate.
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bioRxiv - Bioengineering 2019Quote: ... each sample was mixed in a 1.5mL tube with the appropriate volume of EGFP mRNA (996 nucleotides translated into a 26.9 kDa protein, 1 mg mL−1 L-7601, TriLink BioTechnologies) at final concentrations ranging from 10 μg mL−1 to 160 μg mL−1 (30 nM to 473 nM) ...
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bioRxiv - Genomics 2022Quote: ... 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink ...
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bioRxiv - Molecular Biology 2021Quote: ... or 7-deaza-ATP (TriLink, N-1061-1) during in vitro transcription using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... but with a dNTP mixture in which ATP and CTP were completely substituted with 2-amino-dATP and 5-propynyl-dCTP (TriLink Biotechnologies, San Diego, CA). Template DNA was then precipitated ...
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bioRxiv - Molecular Biology 2020Quote: ... each parental line was transfected with 1 µL of 100 µM sgRNA (Synthego Corporation) and 1 µg Cas9 mRNA (TriLink #L-7206) using the Neon Transfection System following standard protocols ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 mM m1Ψ (TriLink, Cat#N-1019-1). The IVT reaction can be scaled down by a half ...
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bioRxiv - Genomics 2024Quote: ... 1 mM ATP or a modified ATP analog (Trilink, 2’-O-Methyladenosine-5’-Triphosphate ...
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bioRxiv - Developmental Biology 2021Quote: ... Me-CTP (100 mM, TriLink; Cat. No. N-1014-1) and pseudo-UTP (100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and replacing UTP with 1-methylpseudouridine triphosphate (N-1081, TriLink Biotechnologies) as previously described(14) ...
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bioRxiv - Genomics 2023Quote: ... using 5-Methyluridine-5’-Triphosphate (5-mUTP, Trilink, N-1024-1) instead of UTP ...
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bioRxiv - Immunology 2020Quote: ... we first diluted 0.42 μL of the GFP mRNA (Trilink, stored at 1 μg/μl) into 7.26 μL of PBS (pH 5.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) and synthesized in vitro as described in RNA preparation ...
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bioRxiv - Bioengineering 2023Quote: There were two sources for the mRNA used in this study: 1) commercial synthesis by TriLink Biotechnologies (TriLink mRNA was used in Fig ...
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bioRxiv - Immunology 2023Quote: ... for UTP and co-transcriptional capping with CleanCap® Reagent AG (TriLink Biotechnologies, # N-7113-1). For large-scale mRNA production ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 30 pg - 1 ng of eGFP spike-in RNA (TriLink Biotechnologies L-7601-100), vortexed ...
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bioRxiv - Molecular Biology 2021Quote: ... GTP or ATP was substituted with equal molar concentrations of 7-deaza-GTP (TriLink, N-1044-1) or 7-deaza-ATP (TriLink ...
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bioRxiv - Systems Biology 2019Quote: eGFP mRNA and eGFP mRNA with Cy5 label nucleotides (996 nucleotides, 1 mg/mL in 10 mM Tris-HCl and pH 7.5) were purchased from TriLink Biotechnologies ...
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bioRxiv - Molecular Biology 2023Quote: ... with complete substitution of uridine to 1- methylpseudouridine and CleanCap AG analog (N-1081 and N-7113, TriLink Biotechnologies).
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bioRxiv - Synthetic Biology 2024Quote: ... transduced activation reporter cells were enriched by puromycin selection (1µg mL-1) following nucleofection of dCas9-VPR mRNA (TriLink) and a Cas9 sgRNA targeting the ESR protospacer (IDT) ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the single-site RosaBxb-GT host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired attP-GT carrier strains ...
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bioRxiv - Genetics 2021Quote: ... of donor DNA into the dual-site RosaBxb-GT/GA host strains is achieved by microinjection of 1-30ng/µl donor DNA with 100ng/µl Bxb1 mRNA (Trilink) into zygotes of the desired strain ...
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bioRxiv - Bioengineering 2023Quote: ... An organic phase containing a mixture of lipids dissolved in ethanol at a designated molar ratio (Fig 1C and Supp Table 1) was mixed with an aqueous phase (50 mM citrate buffer, pH 4) containing Luciferase mRNA (TriLink) at a flow rate ratio of 1:3 and at a total lipid/mRNA weight ratio of 40:1 in a microfluidic mixing device (NanoAssemblr Ignite ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 104 cells were harvested and incubated in 10 ml Neon Buffer R with 1 ul (1ug/ul) of Clean-Cap Cas9 mRNA (TriLink Biotechnologies) and 1 ul (100 pmol/ul ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) and placed in the 20µl cuvette provided and CB-150 was the Amaxa protocol used ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were electroporated with 1 µg of chemically modified sgRNA (Synthego, Menlo Park, CA) and 1.5 µg codon optimized BE4 mRNA (TriLink Biotechnologies, San Diego, CA) codon optimized ABE7.10 mRNA (TriLink Biotechnologies ...
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bioRxiv - Immunology 2023Quote: ... was prepared in injection buffer by mixing 500 ng/μl of each of the sgRNAs (left and right) with Cas9 mRNA (1 μg/μl, TriLink, cat # L-6125). Fertilized eggs collected from B6/129 mice were microinjected at the CHOP transgenic core and transferred into pseudo-pregnant B6 females ...