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Citations for Megazyme :
101 - 150 of 151 citations for Mouse Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... One third volume of individual sample was used to measure the glucose level with the Glucose Kit (Megazyme; K-GLUC). Another 1/3 volume was used to determine the trehalose level ...
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bioRxiv - Plant Biology 2021Quote: ... leaves decolorized by 80% ethanol and starch concentration was subsequently measured using a starch assay kit (Megazyme, K-TSTA-100A).
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bioRxiv - Plant Biology 2020Quote: The determination of free Asn content in storage roots was carried out with an L-asparagine kit (Megazyme, K-ASNAM). Measurements were carried out according to(Lecart et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots (10 to 50 µl) were analyzed for sucrose and glucose contents with the K-SUFRG Kit (Megazyme, Bray, Ireland) in triplicate measurements in 96-well plates using a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were pelleted and sucrose was measured from the supernatant and lysed pellet fractions using sucrose/D-glucose assay kits (Megazyme). Biological replicates were performed in triplicate.
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bioRxiv - Physiology 2020Quote: ... The analysis of insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) was completed using a Megazyme kit (Total dietary fibre assay procedure, Megazyme International Ireland Ltd. ...
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bioRxiv - Physiology 2020Quote: ... Ingredients were analyzed for total starch using the AOAC method 996.11 and the AACC method 76-13.01 using a Megazyme kit (Total starch assay procedure, Amyloglucosidase/α-amylase method, Megazyme International Ireland Ltd. ...
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bioRxiv - Plant Biology 2021Quote: ... Pellets were dried for 15 min at 55 °C and starch was assayed with HK Assay Kit (Megazyme, Bray, Ireland), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: Analyses of D-glucose and L-lactate concentrations from spent culture medium samples were performed using the D-Glucose-HK and L-Lactic acid kits from Megazyme following manufacturer's protocol ...
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bioRxiv - Microbiology 2022Quote: ... Continuous mannose release was determined using a linked enzyme assay system (D-glucose/D-fructose/D-mannose detection kit, Megazyme), as described in (41 ...
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bioRxiv - Microbiology 2022Quote: ... The concentrations of D-/L-lactate were measured by D- /L-lactic acid Assay Kits according to the manual assay procedure (Megazyme) using ultraviolet spectrophotometry ...
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bioRxiv - Plant Biology 2023Quote: The acetylation of cell wall polymers in MSC surface wall abrasive samples was analyzed directly in the collection tube following adaptation of a previously described method (Stranne et al., 2018) using the K-ACET enzymatic Acetic Acid Assay Kit (Megazyme). The detailed protocol is provided in Supplemental Methods.
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bioRxiv - Synthetic Biology 2024Quote: ... and centrifuged to obtain the supernatant which was used for sucrose measurement using the sucrose/D-glucose assay kit (Megazyme) (10 ...
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bioRxiv - Plant Biology 2024Quote: The total starch content of samples collected daily was determined using Total Starch Assay Kit (K-TSTA-100A, Megazyme, Ireland) as described in its instruction with modifications ...
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bioRxiv - Plant Biology 2024Quote: ... 100 mg flour was used and the content was masured using the Megazyme Total Starch Assay Kit (Megazyme; KTSTA-50A) following manufacturer’s method (three replicates per sample) ...
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bioRxiv - Microbiology 2023Quote: ʟ-arabinose was quantified from homogenised and filtered luminal and colon tissue samples of BALB/c mice using an L-arabinose/D-galactose assay kit (Megazyme) as per the manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... Afterwards the glucose concentration resulting from sucrose hydrolysis by invertase was determined using a D-Glucose assay kit (Megazyme, Bray, Ireland). The glucose production rate was calculated in μMol·min-1·g dry weight-1.
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bioRxiv - Microbiology 2020Quote: The procedures for β-glucan measurement in the cell wall planktonic cells and ECM of biofilm were performed using enzymatic yeast β-glucan kit purchased from Megazyme International (Ireland) ...
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bioRxiv - Plant Biology 2022Quote: ... Organic nitrogen (Norg) values were calculated by subtracting total N levels by spectrophotometrically determined ammonium (Ammonia assay kit, Megazyme, Bray, Ireland) and nitrate levels (Bloom et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated protein was pelleted by centrifugation and the L-lactate concentration was measured using the D-/L-lactate assay kit (Megazyme Inc.), as previously described.3 To examine the reversibility of inactivation ...
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bioRxiv - Biochemistry 2024Quote: The kinetic parameters of the BT4241 GH2 β-galactosidase against disaccharide substrates was determined by the continuous monitoring of galactose release using a D-Gal detection kit (Megazyme International) at 37°C in 20 mM MOPS buffer ...
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bioRxiv - Genetics 2021Quote: ... Glycerol and malic acid concentration were determined by enzymatic assay (Peltier et al. 2018) using K-GCROLGK and K-LMAL-116A enzymatic kits (Megazyme, Bray, Ireland), following the instructions of the manufacturer.
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bioRxiv - Plant Biology 2020Quote: ... five mature grains were milled and used to quantify (1,3;1,4)-β-glucan content using a modified version of the Mixed Linkage β-Glucan Assay Kit (K-BGLU, AACC Method 32-23.01, AOAC Method 995.16, Megazyme Int. (Wicklow, Ireland)) and based on the method described by McCleary & Codd ...
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bioRxiv - Physiology 2021Quote: ... 1/100 or 1/1000 in PBS 1X as follows: lactose content was measured from 200 μl of diluted samples with Lactose/D-Galactose (Rapid) Assay Kit (Megazyme, Wicklow, Ireland) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... acetic acid and ethanol dosages of fermented and unfermented purées were performed with kits from Megazyme (K-FRUGL, K-ACET, K-ETOH).
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged for 10 min at 4 °C and 13000 rpm and the supernatant was collected into a new tube for sugar quantifications that were performed using ©Megazyme Sucrose/D-Fructose/D-Glucose Assay Kit (K-SUFRG, ©Megazyme) with a few modifications ...
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bioRxiv - Bioengineering 2022Quote: Extracellular ammonium in the culture supernatants and medium samples was measured spectrophotometrically using a commercial MegaZyme Ammonium Assay Kit (cat. no. KURAMR, Megazyme, Bray, Ireland) and a standard curve with ammonium chloride (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: D- and L-lactic acid were quantified in cell-free swab supernatants using the sequential method of the D-/L-Lactic Acid Rapid Assay Kit (Megazyme, Darmstadt, Germany). Adapted from the manufacturer’s instructions for a 96 well plate format ...
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bioRxiv - Plant Biology 2022Quote: ... The residue was dissolved in 100 µl ddH2O and a 5-40 µl aliquot was used for analysis in duplicates using the D-Glucose Assay Kit (Megazyme International, Ireland) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... The concentrations of D– and L-lactate isomers were measured in technical duplicates with the D-/L-lactic acid kit (Megazyme, Bray, Ireland). We measured the carboxylates acetate ...
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bioRxiv - Biochemistry 2020Quote: The hydrolysis of α-maltose-1-phosphate was tested using Aspergillus niger amyloglucosidase from Megazyme (high purity for use in Megazyme Total Starch method ...
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bioRxiv - Biochemistry 2021Quote: ... The counterstain was performed by mixing with 25 ug mL-1 Calcofluor (Megazyme C-CLFR) for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... The first step involved manually adding 75 µl of 1% w/v Beechwood xylan (Megazyme) solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultivated at 30 C with shaking (180 rpm) and growth was followed by OD600 and ethanol determined enzymatically with a kit (Megazyme International Ireland Ltd).
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bioRxiv - Plant Biology 2024Quote: Starch content was extracted from 50 mg of dry matter and analyzed using the “Total Starch” enzymatic kit (Megazyme International, County Wicklow, Ireland). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 nM flg22 [u1959ek130 Genescript] or 100 μg mL−1 chitin hexamers(O-ch16 Megazyme) was added to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... phaffii was determined by mixing 1 mg/mL AZCL-HEC substrate (Megazyme, County Wicklow, Bray, Ireland), with 1 mM ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: Free Asparagine content was quantified in 3 months stored potato samples with the help of the L-Asparagine/L-Glutamine/Ammonia kit as described in the assay procedure (Megazyme, K-ASNAM 07/17), with some modifications ...
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bioRxiv - Plant Biology 2020Quote: ... The glucose content of samples and controls was quantified using the K-SUFRG kit as described in the assay procedure (Megazyme, K-SUFRG 04/17), and the amount of glucose produced was determined by calculating the difference in glucose content between samples and controls ...
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bioRxiv - Plant Biology 2020Quote: ... A standard curve for β-1,3-glucan was constructed by using 0–50 mg ml-1 curdlan (Megazyme) dissolved in 0.1 M NaOH and also heating for 30 min at 50 °C in 1 N NaOH.
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bioRxiv - Plant Biology 2023Quote: ... Samples were digested with 1 U/mg DW sample of Aspergillus aculeatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) (Paterlini et al. ...
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bioRxiv - Plant Biology 2023Quote: 10 mg of dried alcohol insoluble cell wall residues were incubated with 10U.ml-1 of endo-1,4-D-xylanase (E-XYLNP) from MEGAZYME previously desalted with centrifulgal filters (Amicon Ultra-0.5ml ...
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bioRxiv - Microbiology 2020Quote: ... α-D-galactose-1-phosphate (Gal1P) and α-L-fucose (Fuc) were form Carbosynth and xylotetraose was from Megazyme. Galactose (Gal) ...
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bioRxiv - Biochemistry 2023Quote: 5 μL of CVF fluid (not centrifuged) was added to 95 μL of 10 mg mL-1 pullulan (Megazyme) in assay buffer (20 mM sodium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... in 100 μL of 0.2 M potassium phosphate buffer (pH 7.0) containing 1 U of endo-1,4-β-Mannanase (Megazyme, E-BMABC). After incubation ...
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bioRxiv - Plant Biology 2021Quote: ... were first subjected to a 2 h digestion in ammonium acetate buffer 100mM pH4 at 40 °C by 2.9 U.mL−1 of Aspergillus acuelatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) to generate OGs which differ in their degrees of polymerization and methylesterification ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cell pellets were resuspended into BMM-C and then inoculated into 5 mL BMM or BMM-xyloglucan media (5 g L-1 xyloglucan, Megazyme) to an initial OD600 of 0.05 ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: L-lactate levels were measured in serum or plasma samples (1:5 diluted in PBS) and in cell culture supernatants (1:10 diluted in PBS) according to manufacturer’s instructions (Megazyme, Wicklow, Ireland). Lactate level in cell culture supernatant were normalized to cell count.
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bioRxiv - Microbiology 2022Quote: Endo-β-1,4-xylanase activity was assayed using a 200 μL mixture of 10 g L−1 beechwood GX (Megazyme, Ireland) in 50 mM sodium acetate buffer (pH 5.5 ...
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bioRxiv - Microbiology 2022Quote: ... vitamins and trace metals as described previously (35) additionally containing either 20 g L−1 (w/v) of beechwood glucuronoxylan (Megazyme, Ireland) or 20 g L−1 xylose (w/v) ...