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Citations for Megazyme :
51 - 89 of 89 citations for 6 chloro 9H 3 C methyl β D ribofuranosyl purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The procedures for β-glucan measurement in the cell wall planktonic cells and ECM of biofilm were performed using enzymatic yeast β-glucan kit purchased from Megazyme International (Ireland) ...
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bioRxiv - Microbiology 2022Quote: Endo-β-1,4-xylanase activity was assayed using a 200 μL mixture of 10 g L−1 beechwood GX (Megazyme, Ireland) in 50 mM sodium acetate buffer (pH 5.5 ...
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bioRxiv - Microbiology 2021Quote: ... For the spectrometric glycogen determination the Sucrose/D-Glucose Assay Kit from Megazyme (K-SUCGL) was applied according to the manufacturer’s specifications ...
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bioRxiv - Plant Biology 2020Quote: ... five mature grains were milled and used to quantify (1,3;1,4)-β-glucan content using a modified version of the Mixed Linkage β-Glucan Assay Kit (K-BGLU, AACC Method 32-23.01, AOAC Method 995.16, Megazyme Int. (Wicklow, Ireland)) and based on the method described by McCleary & Codd ...
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bioRxiv - Genetics 2019Quote: ... and glucose was measured by incubation with 150 μL D-Glucose Assay reagent (Megazyme, K-GLUC) at 37°C for 5 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Secreted sucrose was quantified from supernatants using the Sucrose/D-Glucose Assay Kit (K-SUCGL; Megazyme).
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bioRxiv - Bioengineering 2022Quote: Secreted sucrose was quantified from supernatants using the Sucrose/D-Glucose Assay Kit (K-SUCGL; Megazyme) and converted into cell basis via OD750 standard curve calibration.
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bioRxiv - Biochemistry 2022Quote: ... either by HPLC (see HPLC quantification of glucose) or with the D-Glucose HK Assay kit (Megazyme) following the microplate procedure ...
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bioRxiv - Microbiology 2021Quote: The hydrolysis of scopolin was followed by glucose quantification using the D-Glucose HK Assay kit (Megazyme) following the microplate procedure ...
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bioRxiv - Microbiology 2021Quote: ... Sucrose quantification from culture supernatants was determined using the Sucrose/ d-Glucose Assay Kit (Megazyme: K-SUCGL) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The increase in absorbance at 340 nm after the supplementary addition of 1.2 U of hexokinase and 0.6 U of glucose-6-phosphate dehydrogenase (Megazyme) allowed us to estimate the amount of glucose units from a standard curve ...
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bioRxiv - Zoology 2023Quote: ... at 37°C for 30 min using glucose assay reagent (Megazyme; K-GLUC). We subtracted the amount of free glucose from the measurement and then normalized the subtracted values to protein levels in the supernatant ...
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bioRxiv - Zoology 2023Quote: ... at 37°C for 30 min using glucose assay reagent (Megazyme; K-GLUC) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
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bioRxiv - Plant Biology 2023Quote: 10 mg of dried alcohol insoluble cell wall residues were incubated with 10U.ml-1 of endo-1,4-D-xylanase (E-XYLNP) from MEGAZYME previously desalted with centrifulgal filters (Amicon Ultra-0.5ml ...
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bioRxiv - Microbiology 2023Quote: ... in a 24-well plate (6×4) using 100 mg/L of each resin acid: dehydroabietic acid (Megazyme ∼90%), abietic acid (Sigma ∼75%) ...
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bioRxiv - Microbiology 2022Quote: ... The dissolved agarose solution was used to determine the concentration of glucose according to manufacturer’s instruction (D-Glucose assay kit (GOPOD format, Megazyme).
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bioRxiv - Microbiology 2020Quote: ... α-D-galactose-1-phosphate (Gal1P) and α-L-fucose (Fuc) were form Carbosynth and xylotetraose was from Megazyme. Galactose (Gal) ...
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bioRxiv - Biochemistry 2021Quote: ... The mass spectrometer was calibrated with a mixture of manno-oligosaccharides or xylo-oligosaccharides (DP, 2 to 6) obtained from Megazyme. For sample preparation ...
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bioRxiv - Biochemistry 2021Quote: ... The counterstain was performed by mixing with 25 ug mL-1 Calcofluor (Megazyme C-CLFR) for 5 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were pelleted and sucrose was measured from the supernatant and lysed pellet fractions using sucrose/D-glucose assay kits (Megazyme). Biological replicates were performed in triplicate.
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bioRxiv - Cell Biology 2020Quote: Analyses of D-glucose and L-lactate concentrations from spent culture medium samples were performed using the D-Glucose-HK and L-Lactic acid kits from Megazyme following manufacturer's protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and centrifuged to obtain the supernatant which was used for sucrose measurement using the sucrose/D-glucose assay kit (Megazyme) (10 ...
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bioRxiv - Plant Biology 2022Quote: ... Starch was gelatinised at 95°C and digested to glucose using a mix of amyloglucosidase (Megazyme) and ɑ-amylase (Megazyme) ...
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bioRxiv - Systems Biology 2021Quote: ... Afterwards the glucose concentration resulting from sucrose hydrolysis by invertase was determined using a D-Glucose assay kit (Megazyme, Bray, Ireland). The glucose production rate was calculated in μMol·min-1·g dry weight-1.
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bioRxiv - Biochemistry 2022Quote: ... The precipitated protein was pelleted by centrifugation and the L-lactate concentration was measured using the D-/L-lactate assay kit (Megazyme Inc.), as previously described.3 To examine the reversibility of inactivation ...
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bioRxiv - Biochemistry 2024Quote: The kinetic parameters of the BT4241 GH2 β-galactosidase against disaccharide substrates was determined by the continuous monitoring of galactose release using a D-Gal detection kit (Megazyme International) at 37°C in 20 mM MOPS buffer ...
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bioRxiv - Physiology 2021Quote: ... 1/100 or 1/1000 in PBS 1X as follows: lactose content was measured from 200 μl of diluted samples with Lactose/D-Galactose (Rapid) Assay Kit (Megazyme, Wicklow, Ireland) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: D- and L-lactic acid were quantified in cell-free swab supernatants using the sequential method of the D-/L-Lactic Acid Rapid Assay Kit (Megazyme, Darmstadt, Germany). Adapted from the manufacturer’s instructions for a 96 well plate format ...
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bioRxiv - Plant Biology 2022Quote: ... The residue was dissolved in 100 µl ddH2O and a 5-40 µl aliquot was used for analysis in duplicates using the D-Glucose Assay Kit (Megazyme International, Ireland) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... 10 μg/mL peroxidase from horseradish (Boehringer-Mannheim) and 200 nM of one of the tested elicitors: bacterial Ruminococcus flavefaciens glucuronoyl esterase (RfGCE) (Megazyme 9016-18-6), flg22 (AnaSpec AS-62633) ...
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bioRxiv - Plant Biology 2021Quote: ... were first subjected to a 2 h digestion in ammonium acetate buffer 100mM pH4 at 40 °C by 2.9 U.mL−1 of Aspergillus acuelatus endo-polygalacturonase M2 (Megazyme, Bray, Ireland) to generate OGs which differ in their degrees of polymerization and methylesterification ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cell pellets were resuspended into BMM-C and then inoculated into 5 mL BMM or BMM-xyloglucan media (5 g L-1 xyloglucan, Megazyme) to an initial OD600 of 0.05 ...
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bioRxiv - Plant Biology 2021Quote: ... or in vitro reaction products were digested with Neocallimastix patriciarum GH11 enzyme overnight at 30 °C by amending the suspension with 2 μL of enzyme stock (Megazyme). These conditions achieved complete digestion of accessible xylan to xylose ...
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bioRxiv - Plant Biology 2022Quote: Alcohol-insoluble material from ground seeds or hypocotyls was incubated during 16h at 37°C in 50 mM ammonium formate pH 5 with 5 units of Aspergillus aculeatus endo-polygalacturonase (Megazyme), which hydrolyzed the HG between at least two non-esterified GalA residues and was tolerant to acetyl groups (Voxeur et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Pellets were dried for 15 min at 55 °C and starch was assayed with HK Assay Kit (Megazyme, Bray, Ireland), following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... Tissue sections were removed from the slide surface by incubation for one hour at 37°C in a hydrolytic enzyme mixture consisting of pectate lyase (Megazyme), xyloglucanase (Megazyme) ...
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bioRxiv - Plant Biology 2022Quote: ... Starch in the insoluble fraction of the extraction was gelatinised at 95°C and digested to glucose with α-amylase (Megazyme) and amyloglucosidase (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultivated at 30 C with shaking (180 rpm) and growth was followed by OD600 and ethanol determined enzymatically with a kit (Megazyme International Ireland Ltd).