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Citations for Megazyme :
1 - 50 of 78 citations for 6 Amino 4 hydroxy 3 7 sulfo 4 4 sulfophenyl azo 1 naphthyl azo naphthalene 2 7 disulfonic acid sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 0.1% Azo-Xyloglucan (Megazyme, S-AZXG) or 0.1% polygalacturonic acid in 1M sodium phosphate buffer pH 7.0 (PGA ...
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bioRxiv - Biochemistry 2024Quote: ... AZO-Carob Galactomannan were purchased from Megazyme (Ireland).
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bioRxiv - Genetics 2019Quote: ... Azo-CMCase activity assays were done according to manufacturer’s protocols (Megazyme, Ireland, S-ACMC), slightly modified according to [36].
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bioRxiv - Microbiology 2023Quote: ... in a 24-well plate (6×4) using 100 mg/L of each resin acid: dehydroabietic acid (Megazyme ∼90%), abietic acid (Sigma ∼75%) ...
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bioRxiv - Plant Biology 2023Quote: ... E-XEGP (xyloglucan-specific endo-β-(1→4)-glucanase (Megazyme, Wicklow, Ireland) by adding 10 μl endoglucanase solution (0.4U/μl ...
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bioRxiv - Genetics 2019Quote: ... 80 μg 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) and 0.0025 μg of purified enzyme ...
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bioRxiv - Immunology 2021Quote: β-1,3(1-6) glucan (Yeast Beta Glucan, Megazyme, Bray, Ireland; 3 mg) was suspended in 4.9 mL MilliQ H2O contaning 100 μL of 1 M NaOH ...
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bioRxiv - Genetics 2022Quote: ... 3 mM of chitooligosaccharides (DP 3-6) (Megazyme, Wicklow, Ireland) were treated with 0.12 mg/mL of ChitO (Gecco Biotech ...
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bioRxiv - Genetics 2019Quote: The β-mannopyranosidase activity was then assayed using 4-Nitrophenyl-β-D-mannopyranoside (Megazyme, Ireland, O-PNPBM) as a substrate according to [66] with the following modification ...
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bioRxiv - Bioengineering 2022Quote: ... Xylanase activity was assayed using 100μl lysate and 0.5mM O-PNPX2 substrate (4-nitrophenyl beta-D-xylobioside, Megazyme) or cellPNP substrate (4-nitrophenyl beta-cellobioside ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The incubation was carried out at room temperature for 4 hours and followed by adding GlcNAc5 (A5; Megazyme Cat# O-CHI5) to a final concentration of 0.625 mg/ml and continued the incubation at 37 ℃ for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... Cat.# P0705S); endo-1,4-β-D-glucanase (cellulase from Trichoderma longibrachiatum, glycoside hydrolase family 7; Cat.# E-CELTR) from Megazyme. BsEXLX1 (expansin from Bacillus subtilis ...
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bioRxiv - Microbiology 2022Quote: ... Citric acid kit: Citric Acid Assay Kit (Megazyme, product Code: K-CITR). Phosphate kit ...
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bioRxiv - Microbiology 2023Quote: Growth on dehydroabietic acid and abietic acid was done in cultures of M9 medium containing 100 mg/L of dehydroabietic acid (Megazyme ∼90%) or abietic acid (Sigma ∼75% ...
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bioRxiv - Biochemistry 2021Quote: ... The mass spectrometer was calibrated with a mixture of manno-oligosaccharides or xylo-oligosaccharides (DP, 2 to 6) obtained from Megazyme. For sample preparation ...
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bioRxiv - Plant Biology 2021Quote: ... polygalacturonic acid (Megazyme, Wicklow, Ireland), rhamnogalacturonan (R3875 ...
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bioRxiv - Microbiology 2022Quote: ... mixtures of (GlcNAc)6 (Megazyme) and dialyzed BcLysM1 were incubated for 2 hours at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... the gluconic acid Kit (Megazyme, Ireland) was used ...
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bioRxiv - Microbiology 2022Quote: ... were inoculated separately with a starting OD600= 0.1 or in different ratios (1:1 and 1:10) in Delft medium + 2% beechwood glucuronoxylan (Megazyme, Ireland) for 120 h at RT ...
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bioRxiv - Plant Biology 2023Quote: ... and laminaripentaose β-1-3-(Glc)5 at a concentration of 1.5 mg·mL−1 were used as standards (Megazyme, Bray, Ireland). To visualize the glucan fragments ...
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bioRxiv - Synthetic Biology 2022Quote: ... and Acetic Acid Assay kits (K-ACET, Megazyme), respectively ...
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bioRxiv - Microbiology 2021Quote: ... aureus supernatant using the Citric Acid Assay Kit (Megazyme) according to the microplate assay instructions ...
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bioRxiv - Biochemistry 2023Quote: ... arabinan and polygalacturonic acid were purchased from Megazyme (Wicklow, Ireland)..
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bioRxiv - Systems Biology 2021Quote: ... The pyruvate concentration was estimated using a pyruvic acid kit (Megazyme, Bray, Ireland). The two amino acids in the defined medium ...
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bioRxiv - Synthetic Biology 2022Quote: ... was achieved via enzymatic assay using Succinic Acid Assay Kit (K-SUCC, Megazyme) and Acetic Acid Assay kits (K-ACET ...
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bioRxiv - Genetics 2023Quote: ... and a d-gluconic acid/d-glucono-δ-lactone assay kit (Megazyme International), were used according to the manufacturer’s instructions 1 g of berry pericarp powdered material was diluted in 10 mL of buffer containing 500 µL Carrez 1 solution ...
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bioRxiv - Microbiology 2020Quote: ... 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme) or no amyloglucosidase to then quantify the amount of free glucose and maltose after addition of 100 µL of a buffer (500 mM triethanolamine hydrochloride ...
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bioRxiv - Developmental Biology 2019Quote: Citrate was quantified in medium samples using the Megazyme Citric Acid Kit (Megazyme, Ireland).
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bioRxiv - Neuroscience 2021Quote: ... Three further rounds of centrifugation and suspension in 67% ethanol with 15 mM LiCl followed after which the pellet was resuspended in 100 mM sodium acetate pH 4.5 and digested with amyloglucosidase (Megazyme) in a 55°C oven for 1 hour along with digestion of blank controls ...
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bioRxiv - Plant Biology 2023Quote: ... was added to 90 µL enzyme solution containing 50 mM sodium-phosphate buffer (pH4.5) and endo-β-1,4-galactanase from Aspergillus niger (1.3 units, Megazyme, Ireland) and incubated at 37°C for 12 h ...
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bioRxiv - Plant Biology 2023Quote: ... Activity assays of rTBL38 was performed with the acetic acid assay kit (K-ACETRM, Megazyme) using the activity buffer at 40°C and with three different acetylated substrates ...
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bioRxiv - Biochemistry 2023Quote: EcOpgD (3.6 μg/mL) was incubated in 50 mM sodium acetate buffer (pH 5.0) containing each substrate (0.05% glucomannan, Megazyme (Wicklow, Ireland); 0.0025% polygalacturonic acid ...
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bioRxiv - Microbiology 2023Quote: Supernatant were used to measure acetate concentrations using Acetate assay kit (Megazyme Acetic Acid Assay Kit). The slope of the plot of acetate concentrations versus OD600 for all replicates (multiplied with the measured growth rate ...
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bioRxiv - Microbiology 2022Quote: ... and xylan (2% w/v, Megazyme) were prepared with nanopure water and filter sterilized using 0.4 μm surfactant-free cellulose acetate filters (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... Transaminase activity was tested using an L-glutamic acid detection kit (K-GLUT, Megazyme Inc., Wicklow, Ireland), according to the manufacture’s microplate assay procedure with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... The increase in absorbance at 340 nm after the supplementary addition of 1.2 U of hexokinase and 0.6 U of glucose-6-phosphate dehydrogenase (Megazyme) allowed us to estimate the amount of glucose units from a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... During fermentation, sugars (glucose, fructose) and malic acid consumption were monitored using modified commercial enzymatic kits (Megazyme, Bray, Ireland) as described in (Walker et al. ...
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bioRxiv - Bioengineering 2022Quote: ... Both gluconate and glucono-δ-lactone were quantified using D-Gluconic Acid/D-Glucono-δ-lactone Assay Kit (Megazyme) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The highest mean intensity detected in the anoxic brine core was obtained by the mAb LM19 (anti-homogalacturonan) binding to a polysaccharide standard (polygalacturonic acid (Megazyme) at 0.25 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: Analyses of D-glucose and L-lactate concentrations from spent culture medium samples were performed using the D-Glucose-HK and L-Lactic acid kits from Megazyme following manufacturer's protocol ...
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bioRxiv - Microbiology 2022Quote: ... The concentrations of D-/L-lactate were measured by D- /L-lactic acid Assay Kits according to the manual assay procedure (Megazyme) using ultraviolet spectrophotometry ...
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bioRxiv - Plant Biology 2023Quote: The acetylation of cell wall polymers in MSC surface wall abrasive samples was analyzed directly in the collection tube following adaptation of a previously described method (Stranne et al., 2018) using the K-ACET enzymatic Acetic Acid Assay Kit (Megazyme). The detailed protocol is provided in Supplemental Methods.
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bioRxiv - Biochemistry 2022Quote: ... l-arabino-oligosaccharides (DP = 2-9) obtained commercially (Megazyme) were used as standards at a concentration of 25 μM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... acetic acid and ethanol dosages of fermented and unfermented purées were performed with kits from Megazyme (K-FRUGL, K-ACET, K-ETOH).
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bioRxiv - Microbiology 2022Quote: D- and L-lactic acid were quantified in cell-free swab supernatants using the sequential method of the D-/L-Lactic Acid Rapid Assay Kit (Megazyme, Darmstadt, Germany). Adapted from the manufacturer’s instructions for a 96 well plate format ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrations of D– and L-lactate isomers were measured in technical duplicates with the D-/L-lactic acid kit (Megazyme, Bray, Ireland). We measured the carboxylates acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg/mL peroxidase from horseradish (Boehringer-Mannheim) and 200 nM of one of the tested elicitors: bacterial Ruminococcus flavefaciens glucuronoyl esterase (RfGCE) (Megazyme 9016-18-6), flg22 (AnaSpec AS-62633) ...
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bioRxiv - Plant Biology 2021Quote: ... or in vitro reaction products were digested with Neocallimastix patriciarum GH11 enzyme overnight at 30 °C by amending the suspension with 2 μL of enzyme stock (Megazyme). These conditions achieved complete digestion of accessible xylan to xylose ...
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bioRxiv - Microbiology 2022Quote: ... with a starting OD600= 5 were pipetted onto Delft minimal medium agar plates (2 %) containing 0.4 % beechwood glucuronoxylan (Megazyme, Ireland) or wheat arabinoxylan (Megazyme ...
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bioRxiv - Biochemistry 2023Quote: ... 300 μL reactions were set up containing substrate (0.0048-10 mg mL-1 glycogen; 0.0012-1.25 mg mL-1 Pullulan (Megazyme); or 0.0048-1.25 mg mL-1 amylose in a final concentration of 2% DMSO) ...