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Citations for Biosearch Technologies :
1 - 50 of 55 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 3 ug of total RNA from cultured keratinocytes or fibroblasts was treated without (control) or with 10 units of RNase R in 10X RNase R reaction buffer (LGC Biosearch Technologies, Hoddesdon, UK). Treatment was conducted at 37°C for 12 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were incubated with 125 nM of each probeset (one for histone genes and second one for a control gene FOS in the Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies; SMF-HB1-10) overnight at 37°C in humified chamber ...
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bioRxiv - Genetics 2024Quote: ... QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK, cat.: QE09050) was used to collect genomic DNA (gDNA ...
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bioRxiv - Immunology 2023Quote: ... 50μg NP(30-39)-CGG (Biosearch Tech, cat: N-5055D-5) was mixed with Alum (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... 50μg NP(30-39)-CGG (Biosearch Tech, cat: N-5055D-5) in PBS was mixed with Imject Alum (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat. # 60242). Five hundred thousand colonies were pooled ...
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bioRxiv - Genetics 2024Quote: ... HSPCs were harvested with QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050) to collect gDNA ...
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bioRxiv - Cell Biology 2022Quote: ... and stored in Wash Buffer B (Cat# SMF-WA1-60, LGC Biosearch Technologies) for imaging ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with wash buffer B (Biosearch Technologies, Cat# SMF-WB1-20) and resuspend in a small drop (approximately 30 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with Wash Buffer A (cat. #SMF-WA1-60, Biosearch technologies) for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with Wash Buffer B (cat. # SMF-WB1-20, Biosearch technologies) for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... 20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) in nuclease-free water (Ambion ...
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bioRxiv - Immunology 2023Quote: ... Phosphorylcholine Keyhole Limpet Hemocyanin (PC-KLH, LGC Biosearch Technologies, Cat# PC-1013-5) was applied at a concentration of 100 ng per well ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 50 µl Hybridization Buffer (LGC Biosearch Tech, Cat# SMF-HB1-10) containing RNA probe set overnight at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... and then proceeded for hybridization with 100ul hybridization buffer (Biosearch Technologies, Cat# SMFHB1-10) containing Hmrhl probe (working concentration 125 nM ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Immunology 2023Quote: ... mice were immunized with 100ug of NP(24)-CGG (Cat. N5055C-5, Biosearch technologies) emulsified in alum (Cat ...
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bioRxiv - Developmental Biology 2019Quote: ... Then ovaries were washed with Wash Buffer A (LGC Biosearch Tech, Cat# SMF-WA1-60) at room temperature for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellet was washed once with washed buffer A (Biosearch Technologies, Cat# SMF-WA1-60) and then proceeded for hybridization with 100ul hybridization buffer (Biosearch Technologies ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were incubated in Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies Cat# SMF-HB1-10) with 10% deionized formamide and 125 µM Quasar 640-labeled oligonucleotide probes ...
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bioRxiv - Genetics 2023Quote: ... and finally with Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) for 5 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with 200 μL of wash buffer B (Biosearch Technologies Cat# SMF-WB1-20) and incubated with it at room temperature for 2-5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... A final wash step was performed using Wash buffer B (Biosearch Technologies Cat# SMF-WB1-2) for 5 mins before mounting coverslips with ProLong Gold Anti-Fade reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... then in 1 mL Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) with Hoescht stain at 1:10,000 dilution for 5 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Individual colonies were picked and gDNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). Knock-in was determined by ddPCR and INDELs were determined by ICE analysis as described above.
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). The following primer sequences were used to amplify the CCR5 locus around the sgRNA cut site as previously described:50
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). In-out PCR was performed with 3 forward primers and one reverse primer to selectively amplify knock-in events of the Ibalizumab ...
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bioRxiv - Cancer Biology 2021Quote: ... Dll4 mRNA and Notch1 mRNA probes were mixed with the hybridization buffer (cat. # SMF-HB1-10, Biosearch technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Hybridization mix (200 nM probes in Stellaris hybridization buffer, Biosearch Technologies Cat# SMF-HB1-10 and 10% Formamide) was added after aspirating the wash buffer A and the sample was incubated upside-down facing the buffer at 37°C in the dark for about 18–24 h (within assembled humidified chamber) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL Hybridization Buffer (90 μL Stellaris® RNA FISH Hybridization Buffer (Cat# SMF-HB1-10, LGC Biosearch Technologies), 10 μL deionized formamide ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed/equilibrated in buffer A (Stellaris RNA FISH Wash Buffer A (Biosearch Tech. Cat# SMF-WA1-60) with 10% formamide in water ...
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bioRxiv - Genomics 2021Quote: ... DAPI solution was aspirated and samples were washed once with wash buffer B (Biosearch Technologies Cat# SMF-WB1-20) for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and samples were subsequently incubated with Stellaris® RNA FISH Wash Buffer B (Biosearch Technologies; Cat #: SMF-WB1-20) as prepared through manufacturers protocol for 5 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then washed twice with Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) with the second wash left to incubate for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Evolutionary Biology 2020Quote: ... respectively and a commercially available library of 48 probes was used to detect HOXA5 (cat # VSMF-2538-5) (Stellaris RNA FISH probes, Biosearch Technologies). Hybridization conditions and imaging were as previously described (Itzkovitz et al ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μL Wash Buffer A (40 μL Stellaris® RNA FISH Wash Buffer A (Cat# SMF-WA1-60, LGC Biosearch Technologies), 20 μL deionized formamide ...
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was then removed and 1 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) with 10% deionized formamide for 5 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... followed by pre-hybridized in Wash Buffer A (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) and 10% deionized formamide ...
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the pellet from each sample in 750 mL of chilled resuspension buffer (1X PBS, 0.4 mg/mL BSA, 0.2 U/μL RNAse Inhibitor [Biosearch Technologies, Cat. 30281]) containing one of 8 unique XPoSE-tags (Nucleoporin 62 antibody conjugated to R718 fluorescent dye and one of eight distinct oligo-based Sample Tags (ST ...
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bioRxiv - Molecular Biology 2021Quote: ... and samples were subsequently washed with 500 uL of Stellaris® RNA FISH Wash Buffer B (Biosearch Technologies; Cat #: SMF-WB1-20) as prepared through manufacturers and incubated with 100 uL of Stellaris® RNA FISH Wash Buffer B protocol for 5 minutes at RT ...
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bioRxiv - Genomics 2022Quote: ... The coverslips with cells were immersed in 100µl hybridization buffer (90µl Stellaris RNA-FISH Hybridization Buffer (Biosearch Technologies, Cat. SMF-HB1-10) and 10µl deionized formamide (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: A modified DNA extraction protocol was used to produce high molecular weight DNA based on the Lucigen/EpiCentre’s MasterPureTM Complete DNA and RNA Purification Kit A (Biosearch Technologies, Cat MC85200). For HG002 cell line ...
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bioRxiv - Neuroscience 2020Quote: ... Hybridization followed the manufacturer’s instructions (http://www.biosearchtech.com/stellarisprotocols) and was performed at 37°C for 16h in Stellaris RNA FISH hybridization buffer (Biosearch Technologies Cat# SMF-HB1-10) containing unc-8 probe at 1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1-3 μL of HIV-1 RNA probe diluted in hybridization buffer (Stellaris RNA FISH Hybridization Buffer (Biosearch Tech. Cat# SMF-HB1-10)) and 10% formamide were spotted on coverslips and incubate overnight in humid conditions at 37C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of the TdT-labeled probes was added to 98 μL of Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies Cat# SMF-HB1-10); 100 μL of this mixture was dotted onto the Parafilm in the humidifying chamber ...
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bioRxiv - Molecular Biology 2019Quote: ... and nuclear counterstaining was performed using 1:10000 Hoechst 33342 in buffer A followed by two washings in buffer B (Stellaris RNA FISH Wash Buffer B (Biosearch Tech. Cat# SMF-WB1-20) and mounting the coverslips with mowiol.
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bioRxiv - Developmental Biology 2019Quote: ... ovaries were washed twice with Wash Buffer A at 37 °C for 30 minutes and once with Wash Buffer B (LGC Biosearch Tech, Cat# SMF-WB1-20) at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Ethanol 70% is discarded and cells washed with 200 μL wash buffer A (10% vol./vol. formamide in 1X Wash Buffer A, Biosearch Technologies Cat# SMF-WA1-60). Cells were incubated with 100 μL Hybridization buffer containing 1,25 μM of RNA-FISH probes in the dark at 37 °C overnight (~16 hours) ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess buffer was removed and 100 uL of freshly prepared Hybe Buffer Mixture was added (for two color in situs: 85.5 uL of Stellaris® RNA FISH Hybridization Buffer (Biosearch Technologies; Cat #: SMF-HB1-10); 9.5 uL deionized formamide ...