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Citations for Biosearch Technologies :
51 - 100 of 108 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were washed once with wash buffer A (Biosearch Technologies, SMF-WA1-60) supplemented with 10% deionized formamide (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies) containing Stellaris RNA FISH probes (Biosearch Technologies ...
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bioRxiv - Genomics 2022Quote: ... Cells were subsequently hybridized with 100 μl of Hybridization Buffer (LGC Biosearch Technologies) containing the smRNA-FISH probes at a 1:100 dilution in a humid chamber at 37°C o/n (not more than 16 h) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were washed in Wash Buffer B (Biosearch Technologies SMF-WB1-20) for 5 minutes at room temperature in the dark ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The cells were sorted to rich recovery medium (LGC, Biosearch Technologies, Hoddesdon, UK) in a 1.5 ml centrifugation tube ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaned assembly reactions were electroporated into Endura Electrocompetent cells (Biosearch Technologies 60242-1) according to manufacturer’s instructions and plated on LB-Lennox 250 mm x 250 mm square bioassay dishes supplemented with 75 µg/mL carbenicillin ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with TNP(65)-FL-AECM-Ficoll (20μg/ml; Biosearch Technologies Inc.) prior to performing extracellular staining ...
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bioRxiv - Genomics 2022Quote: ... Cells were then washed with 1 ml of Wash Buffer B (LGC Biosearch Technologies) at RT for 5 min ...
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bioRxiv - Genomics 2023Quote: ... The assembled products were electroporated into Endura electrocompetent Escherichia coli cells (Biosearch Technologies, 60242) and approximately 4,000 independent bacterial colonies were cultured for each library ...
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bioRxiv - Genomics 2023Quote: ... The resulting library was electroporated into Endura electrocompetent Escherichia coli cells (Biosearch Technologies, 60242) and cultured as described above ...
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bioRxiv - Genomics 2023Quote: ... The ligated products were electroporated into Endura electrocompetent Escherichia coli cells (Biosearch Technologies, 60242), and about 40 million bacterial colonies were cultured for each library ...
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bioRxiv - Genomics 2023Quote: ... The assembled products were electroporated into Endura electrocompetent Escherichia coli cells (Biosearch Technologies, 60242). About 25 million bacterial colonies were cultured for each library ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then resuspended in 1 mL wash buffer B (Biosearch Technologies, SMF-WB1-20), incubated for 2-5 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized cells were then resuspended in 100 μL hybridisation solution (Biosearch Technologies, SMF-HB1-10) containing 1-3x of standard probe concentrations for WHI5 and MDN1 probes ...
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bioRxiv - Microbiology 2019Quote: ... Then the cells were washed briefly with Stellaris RNA FISH wash buffer B (Biosearch Technologies), rinsed three times with PBS and subject to imaging by deconvolution microscopy (DeltaVision OMX SR imaging system) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed twice in Stellaris RNA FISH wash buffer A (Biosearch Technologies) with the presence of Hoechst stain during the second wash ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellet was washed once with washed buffer A (Biosearch Technologies, Cat# SMF-WA1-60) and then proceeded for hybridization with 100ul hybridization buffer (Biosearch Technologies ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with 1 ml of Wash buffer A (Biosearch Technologies, SMF-WA1-60). To probe for S4 mRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with Wash Buffer B (Biosearch Technologies, Inc., SMF-WA1-60) for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed and labelled using the Stellaris RNA FISH simultaneous labelling protocol (Biosearch technologies). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were hybridized with 80 μl of hybridization buffer (LGC Biosearch Technologies, SMF-HB1-10) supplemented with 10% deionized formamide containing the FISH probes at a 1:100 dilution at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies, catalog # C400CH10) for low-copy growth in absence of CopyCutter Induction Solution ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with 200 μL of wash buffer B (Biosearch Technologies Cat# SMF-WB1-20) and incubated with it at room temperature for 2-5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells were then incubated with S4 probes diluted in Hybridization buffer (Biosearch Technologies, SMF-HB1-10) in a humidified chamber at 37°C for 4 h in the dark ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 16 hours with FISH probes in the hybridization buffer (LGC Biosearch Technologies). Cells were then washed using wash buffer A (LGC Biosearch Technologies ...
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bioRxiv - Immunology 2023Quote: NP-specific memory B cells were identified by staining with biotinylated NIP(15)-BSA (Biosearch Technologies) at 330 ng/ml for 30 min on ice and then with streptavidin APC ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-molecule RNA FISH was performed according to the protocol for adherent cells provided by Biosearch technologies. Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). The following primer sequences were used to amplify the CCR5 locus around the sgRNA cut site as previously described:50
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). In-out PCR was performed with 3 forward primers and one reverse primer to selectively amplify knock-in events of the Ibalizumab ...
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bioRxiv - Genetics 2023Quote: ... and then resuspended with 100 µL per 1 million cells of QuickExtract (Pt#SS000035-D1 Biosearch Technologies). The hiPSCs were then run in a thermocycler for three consecutive cycles ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with 10% v/v formamide in Stellaris® RNA FISH wash buffer A (Biosearch Technologies) and incubated in 1 mL of DAPI staining solution (5 ng/mL DAPI in wash buffer A with 10% v/v formamide ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed/equilibrated in buffer A (Stellaris RNA FISH Wash Buffer A (Biosearch Tech. Cat# SMF-WA1-60) with 10% formamide in water ...
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bioRxiv - Microbiology 2022Quote: Experiments were performed with slight modifications to the Stellaris protocol for simultaneous FISH-immunofluorescence in adherent cells (Biosearch Technologies)35–37 ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were washed with PBS and DNA extracted using QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ∼5x107 cells were collected and total RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with Wash buffer A (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies, Inc., SMF-WA1-60), 10% Deionized Formamide (EMD Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Genomics 2022Quote: smRNA-FISH experiments were performed according to the Stellaris® RNA FISH protocol for adherent cells provided by Biosearch Technologies. Specifically ...
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bioRxiv - Microbiology 2023Quote: ... Single molecule FISH (smFISH) was performed using Stellaris™ RNA FISH protocol in Adherent Cells (Biosearch Technologies, Novato, CA, USA). After fixation ...
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bioRxiv - Genomics 2022Quote: ... and 2.5 µL of the reaction was transformed into 25 µL of Lucigen Endura ElectroCompetent Cells (Biosearch Technologies, Hoddesdon, UK). Before culture for maxiprep ...
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bioRxiv - Neuroscience 2021Quote: ... single cell samples were prepared for 3’ mRNA sequence determinations using the Scipio bioscience protocol (to be published) with barcoding beads (NxSeq Single-cell RNA-seq Beads, LGC Biosearch Technologies). For beads that captured mRNA molecules ...
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bioRxiv - Molecular Biology 2023Quote: ... Hybridization step occurred in a parafilm-sealed humidity chamber with cells incubated in 125 nM RNA FISH probes (Supplementary Table 3)-containing Stellaris Hybridization Buffer (Biosearch Tech) for 16 hrs at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... the glass coverslips were transferred to a fresh 6-well plate with the cell side up and incubated with Wash Buffer A (Biosearch Technologies) and Hoechst stain (1:2000 ...
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bioRxiv - Genomics 2024Quote: ... The assembled plasmid pool was purified and concentrated with AMPure XP SPRI Reagent and used for transformation of Electrocompetent Endura Cells (#60242-2, Biosearch Technologies) following manufacturer’s instructions and a previously published protocol47 ...
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bioRxiv - Genetics 2024Quote: ... arrested cells were released into rich media containing 200 mM hydroxyurea and 130 μM Edu (5-Ethynyl-2’-deoxyuridine) (Biosearch Technologies). Cells were harvested in G1 and after 60 minutes release into HU ...
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bioRxiv - Genomics 2024Quote: ... Purified ligations were electrotransformed by combining 5 µL of ligation and 25 µL of Endura electrocompetent cells (Biosearch Technologies 60242-2) in a 0.1 cm Gene Pulser cuvette (Biorad 1652083 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were further washed in Wash Buffer I (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies, Inc., SMF-WA1-60), 10% Deionized Formamide (Invitrogen ...