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Citations for Biosearch Technologies :
1 - 50 of 88 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with 10% deionized formamide in 2X SSC for 30 min at 37°C and once with Wash B (Biosearch Technologies) for 5 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Hybridization followed the manufacturer’s instructions (http://www.biosearchtech.com/stellarisprotocols) and was performed at 37°C for 16h in Stellaris RNA FISH hybridization buffer (Biosearch Technologies Cat# SMF-HB1-10) containing unc-8 probe at 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... the coverslips were incubated in a humidified chamber at 37 °C for 14 hours with probes diluted in Stellaris RNA FISH Hybridisation Buffer (LGC Biosearch Technologies; with 10% (v/v) formamide added ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
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bioRxiv - Microbiology 2019Quote: ... with a 7-hour hybridization at 30°C of the custom Stellaris™ (Biosearch Technologies) probes labeled with Quasar® 670 dyes for the SANTV RNA1 molecule and with Cal Fluor Red® 610 Dyes for the LEBV RNA1 molecule (Table S1b).
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with NP3– or NP14–bovine serum albumin (BSA; Biosearch Technologies), diluted in carbonate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... at 37°C and then once with Stellaris wash buffer B (Biosearch technologies SMF-WB1-20) for three minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Coverslips were washed twice for 30 min at 37°C with Stellaris RNA FISH Wash Buffer A (Biosearch Technologies), with 0.2 mg/ml Dapi being added to the second wash ...
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bioRxiv - Bioengineering 2023Quote: ... the MBP-C PNP and its variants were transformed into the lipopolysaccharide defective ClearColi BL21(DE3) strain (Biosearch Technologies), and induced by 1 mM IPTG for 16-20 hours at 20 °C ...
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bioRxiv - Genomics 2024Quote: ... and recovering for 90 minutes at 30 °C in 1 mL of Endura recovery media (Biosearch Technologies 60242-2). Cultures were then grown for 16 hours in 50 mL of 2xYT media with 100 µg/mL of carbenicillin ...
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bioRxiv - Synthetic Biology 2023Quote: ... the RNA strands were transcribed in vitro at 37°C using 7.5% (v/v) T7 polymerase from the AmpliScribe T7-Flash transcription kit (ASF3507, Biosearch Technologies), 40mM of Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos underwent heat shock and were hybridized for 2 h at 38.5 °C in a humidity chamber with XIST Quasar 570 (125 nM; SMF-2038-1; BioSearch Technologies) in a hybridization buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... a set of oligonucleotides spanning the first intron of let-7-C was designed using the Stellaris Probe Designer software (Biosearch Technologies). The oligos were ordered from Biosearch Technologies labeled with Quasar-670.
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bioRxiv - Genomics 2020Quote: ... followed by hybridization overnight at 37°C with 250 nM of each FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Molecular Biology 2019Quote: ... dishes were incubated with Wash Buffer A for 30 minutes at 37°C followed by addition and incubation of Wash Buffer B (LGC Biosearch Technologies) for 5 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... was incubated for 5 minutes and ACTB probe set was hybridized in for 16 hours at 37°C with Hybridization buffer (LGC Biosearch Technologies). After hybridization ...
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bioRxiv - Immunology 2021Quote: ... for ELISPOT assays were prepared with 35% ethanol and then coated overnight at 4 °C with TNP-BSA or NP-BSA (Biosearch Technologies). Plates were washed and subsequently blocked at 37 °C with complete culture media prior to plating and culturing splenocytes or bone marrow cells for 18 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... containing Dapi for 30 minutes at 37°C and then once with Stellaris wash buffer A (Biosearch technologies SMF-WA1-60) containing 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Genomics 2021Quote: ... followed by hybridization for 6 hours to overnight at 37 °C with 250 nM of each FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight hybridization at 37 °C with 250 nM of FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Genomics 2022Quote: ... Cells were then incubated in 70% ethanol at 4°C for at least 1 h and then washed with 1 ml of Wash Buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... After fixation with 4% PFA cells were permeabilized by incubation in 70% EtOH for at least 1 hr at 4°C followed by incubation in Stellaris Wash Buffer A (Biosearch Tech) for 5 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Fixed worms were stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting microsporidia ribosomal RNA (81 ...
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bioRxiv - Immunology 2023Quote: ... Fixed worms were washed and then stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting Orsay virus RNA1 and RNA2 (80 ...
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bioRxiv - Genomics 2023Quote: ... Cover glasses were incubated in 70% ethanol at 4°C for at least one hour and then washed with 1 mL of wash buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Immunology 2024Quote: ... worms were incubated at 47 °C overnight with fluorescein (FAM)- or Quasar 670-conjugated FISH probes that target Orsay virus RNA1 and RNA2 (Biosearch Technologies). Infection rate was assessed using a Zeiss AxioImager M1 compound microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... then incubated overnight at 37°C in the dark with 50 nM of Quasar® 570 labeled Stellaris probe against dpp mRNA (LGC Biosearch Technologies, kind gift from Michael Buszczak ...
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bioRxiv - Cell Biology 2023Quote: ... 300 uL of cells were spun down and hybridized overnight at 30 °C with 100 uL of Stellaris® RNA FISH hybridization buffer (Biosearch Technologies) containing 10% v/v formamide and 125 mM smFISH probes ...
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bioRxiv - Systems Biology 2022Quote: ... incubated at 4°C for 1 h and then washed with 200 μl of wash buffer A (LGC Biosearch Technologies, SMF-WA1-60) supplemented with 10% deionized formamide (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... ovaries were washed twice with Wash Buffer A at 37 °C for 30 minutes and once with Wash Buffer B (LGC Biosearch Tech, Cat# SMF-WB1-20) at room temperature for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... were immunized for a total of 7 times over 28 days with recombinant ovalbumin (Biosearch Technologies) as a model antigen ...
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bioRxiv - Cell Biology 2022Quote: Human MYC_intron with Quasar 570 dye (Biosearch Technologies, ISMF-2066-5), human ACTB_intron with Quasar 570 dye (Biosearch Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... human ACTB_intron with Quasar 570 dye (Biosearch Technologies, ISMF-2002-5), Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies ...
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bioRxiv - Biophysics 2020Quote: ... BHQ-10 maleimide was prepared from BHQ-10 succinimidyl ester (Biosearch Technologies) as described for the related BHQ-3 molecule (36) ...
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bioRxiv - Genomics 2021Quote: Hybridization mix (200 nM probes in Stellaris hybridization buffer, Biosearch Technologies Cat# SMF-HB1-10 and 10% Formamide) was added after aspirating the wash buffer A and the sample was incubated upside-down facing the buffer at 37°C in the dark for about 18–24 h (within assembled humidified chamber) ...
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bioRxiv - Molecular Biology 2019Quote: ... to BHQ-10 succinimidyl ester (Biosearch Technologies). In a typical reaction ...
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bioRxiv - Genomics 2020Quote: ... ChIRP probes (37 x 19-20 nucleotides) targeting LETR1 were designed using the Stellaris Probe Designer (LGC Biosearch Technologies). As non-specific control ...
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bioRxiv - Immunology 2023Quote: Mice were immunized by subcutaneous injection into the hind footpad of 10 μg OVA or 10 μg NP-OVA (Biosearch Technologies) adsorbed in alum (Imject Alum ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA targets were detected in embryos using smiFISH probes designed to exonic sequence with 5’ end X flap sequences [78] and using secondary detection probes labelled with Quasar 570 or Quasar 670 fluorophore dyes (LGC Biosearch Technologies). Probe sequences are listed in Supplementary Table 2 ...
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bioRxiv - Immunology 2024Quote: ... were coated with 25 µl of 10 µg/ml NP-BSA in PBS (N-5050XL-10-BS with loading ratio of 2 or N-5050H-10-BS with loading ratio of 36, both Biosearch Technologies/BioCat) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted in Hybridization Buffer (Biosearch Technologies SMF-HB1-10) plus 10% Deionized Formamide (Millipore 4610) ...
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bioRxiv - Developmental Biology 2021Quote: ... before adding 200μl hybridization buffer (Biosearch Technologies, SMF-HB1-10) containing the U1 probe and covering the tissue with a glass coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies, SMF-HB1-10), Stellaris RNA FISH Wash Buffer A (Biosearch Technologies ...
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bioRxiv - Cell Biology 2023Quote: Xist RNA was detected with Stellaris® RNA FISH (Human XIST with Quasar® 570 Dye, Biosearch Technologies, SMF-2038-1) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 10 μg/ml trinitrophenyl (TNP)-specific human IgG1-4 (clone 7B4) were incubated with 5 μg/ml TNP-conjugated BSA (TNP-33-BSA, BioSearch Technologies) in PBS for 3 hours gently shaking at 60 rpm ...
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bioRxiv - Genomics 2023Quote: ... FLAPY probes were labeled with Cal Fluor 610 and FLAP X probes were labeled with Quasar 670 (Biosearch Technologies, BNS-5082 and FC-1065, respectively). All samples were imaged on a DeltaVision Elite inverted microscope described above ...
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bioRxiv - Immunology 2021Quote: ... or intravenously with 50 μg NP-Ficoll (Biosearch Technologies; F-1420-10). For influenza infections ...
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bioRxiv - Immunology 2022Quote: ... were coated with NP3-BSA or NP14-BSA (10 mg/ml, Biosearch Technologies) in carbonate buffer (0.1 M NaHCO3 ...
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bioRxiv - Immunology 2020Quote: ... mice were immunized with 10 μg NP(19)-OVA (NP-OVA, Biosearch Technologies) absorbed in alum or 25 μg NP-OVA ...
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bioRxiv - Immunology 2022Quote: ... chimeric mice were immunized intravenously with 10 μg/ml NP-Ficoll (Biosearch Technologies) in 100 μl PBS ...