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Citations for Biosearch Technologies :
1 - 50 of 59 citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... the RNA strands were transcribed in vitro at 37°C using 7.5% (v/v) T7 polymerase from the AmpliScribe T7-Flash transcription kit (ASF3507, Biosearch Technologies), 40mM of Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: Probe sets targeting the 3’UTR of rat Atf3 (Stellaris probes, Biosearch technologies) were designed using the Stellaris probe-set designer tool to specifically detect the 3’UTR of the transcript and 3’end labelled with CalFluor590 ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
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bioRxiv - Microbiology 2019Quote: ... with a 7-hour hybridization at 30°C of the custom Stellaris™ (Biosearch Technologies) probes labeled with Quasar® 670 dyes for the SANTV RNA1 molecule and with Cal Fluor Red® 610 Dyes for the LEBV RNA1 molecule (Table S1b).
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with NP3– or NP14–bovine serum albumin (BSA; Biosearch Technologies), diluted in carbonate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... at 37°C and then once with Stellaris wash buffer B (Biosearch technologies SMF-WB1-20) for three minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Coverslips were washed twice for 30 min at 37°C with Stellaris RNA FISH Wash Buffer A (Biosearch Technologies), with 0.2 mg/ml Dapi being added to the second wash ...
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bioRxiv - Bioengineering 2023Quote: ... the MBP-C PNP and its variants were transformed into the lipopolysaccharide defective ClearColi BL21(DE3) strain (Biosearch Technologies), and induced by 1 mM IPTG for 16-20 hours at 20 °C ...
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bioRxiv - Genomics 2024Quote: ... and recovering for 90 minutes at 30 °C in 1 mL of Endura recovery media (Biosearch Technologies 60242-2). Cultures were then grown for 16 hours in 50 mL of 2xYT media with 100 µg/mL of carbenicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos underwent heat shock and were hybridized for 2 h at 38.5 °C in a humidity chamber with XIST Quasar 570 (125 nM; SMF-2038-1; BioSearch Technologies) in a hybridization buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... a set of oligonucleotides spanning the first intron of let-7-C was designed using the Stellaris Probe Designer software (Biosearch Technologies). The oligos were ordered from Biosearch Technologies labeled with Quasar-670.
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bioRxiv - Genomics 2020Quote: ... followed by hybridization overnight at 37°C with 250 nM of each FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Molecular Biology 2019Quote: ... dishes were incubated with Wash Buffer A for 30 minutes at 37°C followed by addition and incubation of Wash Buffer B (LGC Biosearch Technologies) for 5 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... was incubated for 5 minutes and ACTB probe set was hybridized in for 16 hours at 37°C with Hybridization buffer (LGC Biosearch Technologies). After hybridization ...
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bioRxiv - Immunology 2021Quote: ... for ELISPOT assays were prepared with 35% ethanol and then coated overnight at 4 °C with TNP-BSA or NP-BSA (Biosearch Technologies). Plates were washed and subsequently blocked at 37 °C with complete culture media prior to plating and culturing splenocytes or bone marrow cells for 18 h at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... containing Dapi for 30 minutes at 37°C and then once with Stellaris wash buffer A (Biosearch technologies SMF-WA1-60) containing 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with 10% deionized formamide in 2X SSC for 30 min at 37°C and once with Wash B (Biosearch Technologies) for 5 min at RT ...
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bioRxiv - Genomics 2021Quote: ... followed by hybridization for 6 hours to overnight at 37 °C with 250 nM of each FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight hybridization at 37 °C with 250 nM of FISH probe in 50 μl Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies) containing 10% formamide ...
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bioRxiv - Genomics 2022Quote: ... Cells were then incubated in 70% ethanol at 4°C for at least 1 h and then washed with 1 ml of Wash Buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... After fixation with 4% PFA cells were permeabilized by incubation in 70% EtOH for at least 1 hr at 4°C followed by incubation in Stellaris Wash Buffer A (Biosearch Tech) for 5 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Fixed worms were stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting microsporidia ribosomal RNA (81 ...
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bioRxiv - Immunology 2023Quote: ... Fixed worms were washed and then stained at 46 °C overnight using FISH probes conjugated to the red Cal Fluor 610 fluorophore (Biosearch Technologies), targeting Orsay virus RNA1 and RNA2 (80 ...
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bioRxiv - Genomics 2023Quote: ... Cover glasses were incubated in 70% ethanol at 4°C for at least one hour and then washed with 1 mL of wash buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Immunology 2024Quote: ... worms were incubated at 47 °C overnight with fluorescein (FAM)- or Quasar 670-conjugated FISH probes that target Orsay virus RNA1 and RNA2 (Biosearch Technologies). Infection rate was assessed using a Zeiss AxioImager M1 compound microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... then incubated overnight at 37°C in the dark with 50 nM of Quasar® 570 labeled Stellaris probe against dpp mRNA (LGC Biosearch Technologies, kind gift from Michael Buszczak ...
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bioRxiv - Cell Biology 2023Quote: ... 300 uL of cells were spun down and hybridized overnight at 30 °C with 100 uL of Stellaris® RNA FISH hybridization buffer (Biosearch Technologies) containing 10% v/v formamide and 125 mM smFISH probes ...
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bioRxiv - Immunology 2021Quote: ... n2019-nCoV (Biosearch technologies, KIT-NCOV-PP1-1000). For subgenomic N-gene quantification ...
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bioRxiv - Neuroscience 2020Quote: ... Hybridization followed the manufacturer’s instructions (http://www.biosearchtech.com/stellarisprotocols) and was performed at 37°C for 16h in Stellaris RNA FISH hybridization buffer (Biosearch Technologies Cat# SMF-HB1-10) containing unc-8 probe at 1:100 ...
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bioRxiv - Systems Biology 2022Quote: ... incubated at 4°C for 1 h and then washed with 200 μl of wash buffer A (LGC Biosearch Technologies, SMF-WA1-60) supplemented with 10% deionized formamide (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: The method described here depends on the activity of EZ-Tn5 Transposase (EZ-Tn5 is commonly sold in kits for specific purposes. The enzyme by itself, not part of a kit can be purchased from LGC Biosearch Technologies, Petaluma, CA). This enzyme only requires a 19-nucleotide inverted repeat at each end of a linear blunt-end DNA fragment to catalyze the random insertion of the DNA fragment into a target DNA ...
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bioRxiv - Developmental Biology 2019Quote: ... ovaries were washed twice with Wash Buffer A at 37 °C for 30 minutes and once with Wash Buffer B (LGC Biosearch Tech, Cat# SMF-WB1-20) at room temperature for 5 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... the coverslips were incubated in a humidified chamber at 37 °C for 14 hours with probes diluted in Stellaris RNA FISH Hybridisation Buffer (LGC Biosearch Technologies; with 10% (v/v) formamide added ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was polyadenylated with Poly(A) Polymerase Tailing Kit (BIOSEARCH TECHNOLOGIES). Then the polyadenylated product was reversely transcribed into cDNA using a poly(T ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification kit (LGC Biosearch Technologies) and quantified by NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the MasterPure Gram-Positive DNA purification Kit (Biosearch Technologies) with a modified protocol that included a bead-beating step [53] ...
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies, Middleton, WI, USA) and quantified on the QIAxpert® spectrophotometer (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted from EVs using the MasterPure Complete DNA and RNA Purification Kit (Biosearch Technologies). For comparison purposes ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenised material was then treated following the Sbeadex® plant mini kit protocol (LGC Biosearch Technologies, Beverley, MA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Fmr1 mutants was reserve transcribed to cDNA using MMLV High-Performance Reverse Transcriptase Kit (Biosearch Technologies #RT80125K) and oligo(dT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was prepared from 0.5 ml of culture with the MasterPure Yeast DNA Purification kit (Biosearch Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Pure lactobacilli isolates had their DNA extracted using MasterPure™ Gram Positive Purification Kit (LGC Biosearch Technologies, Hoddesdon, UK). All DNA extraction quantifications were performed with Qubit Fluorometer (Thermo Fischer Scientific ...