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Citations for Biosearch Technologies :
1 - 50 of 116 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Ninety-six-well plates were coated with 1 μg/ml NP-BSA (Biosearch Technologies) in bicarbonate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... KASP assay (LGC Biosearch Technologies) was performed (55).
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bioRxiv - Developmental Biology 2021Quote: ... we used KASP assays (Biosearch Technologies). These use allele-specific PCR primers ...
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bioRxiv - Microbiology 2020Quote: ... The probes for the coronavirus assays (E/IP4) were both labelled with a FAM reporter and black hole quencher 1 (Biosearch Technologies) and the XIPC probe with a Vic reporter and TAMRA quencher (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... KASP assays were designed and produced by Biosearch Technologies based on provided sequence data for six locations distributed across the region of low recombination on chromosome 5 ...
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bioRxiv - Microbiology 2022Quote: ... genotyping was performed using the KASP assay (LGC Biosearch technologies). For irf8 mutants and gpr84 crispants ...
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bioRxiv - Molecular Biology 2020Quote: ... we used the competitive allele-specific PCR (KASP™) assay (LGC, Biosearch Technologies) to genotype the zebrafish.
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bioRxiv - Immunology 2023Quote: ... 96-well plates were coated with NP30-BSA (bovine serum albumin) (Biosearch Technologies) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 96-well ELISA plates (42592, Costar) were coated with NP23-BSA (Biosearch Technologies) in PBS overnight and washed ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping PCR were done by using KASP genotyping assay (Biosearch Technologies, Hoddesdon, United Kingdom). A similar workflow was used in genotyping adult carrier fish.
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bioRxiv - Immunology 2019Quote: ... Plates were coated with 10 μg/mL NP31-bovine serum albumin (BSA, Biosearch Technologies) and blocked with PBS 3 % BSA ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with NP29-BSA (low affinity) or NP4-BSA (high affinity) (Biosearch Technologies). For ANA ELISAs ...
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bioRxiv - Neuroscience 2019Quote: ... and vesicular glutamate transporter (vGluT) were designed using the RealTimeDesign™ qPCR Assay Design software (LGC Biosearch Technologies). Primer pairs and probes for the genes of interest were designed to be run in a single qPCR reaction ...
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bioRxiv - Immunology 2023Quote: ... Half-area flat-bottom plates were coated with 10 µg/mL of NP2 or NP25 -BSA (Biosearch Technologies) or 5 µg/mL of goat anti-mouse IgG/IgM (Southern Biotech ...
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bioRxiv - Molecular Biology 2019Quote: ... A FAM-labelled assay was used to amplify a region contained within the ssDNA donor (sourced from Biosearch Technologies), in parallel with a VIC-labelled reference gene assay (Dot1l ...
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bioRxiv - Genetics 2022Quote: ... individual embryos were separated by cryaa:egfp expression and subsequently genotyped for gata2aum27 using a KASP assay as previously (Biosearch Technologies) (Shin et al. ...
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bioRxiv - Immunology 2023Quote: ... All- or high-affinity hapten-specific Ab were measured using plates coated with NP20-BSA or NP2-PSA (Biosearch Technologies), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ...
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bioRxiv - Immunology 2021Quote: ... for ELISPOT assays were prepared with 35% ethanol and then coated overnight at 4 °C with TNP-BSA or NP-BSA (Biosearch Technologies). Plates were washed and subsequently blocked at 37 °C with complete culture media prior to plating and culturing splenocytes or bone marrow cells for 18 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... 1 mL of recovery media (Biosearch Technologies, 80026-1) was transferred to each culture tube in advance ...
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bioRxiv - Immunology 2023Quote: ... The titers of NP-specific antibody isotypes in sera were measured by ELISA using plates coated with NP(7)- BSA or NP(20)-BSA (Biosearch Technologies) as described previously (63).
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bioRxiv - Cell Biology 2023Quote: ... the glass coverslips were transferred to a fresh 6-well plate with the cell side up and incubated with Wash Buffer A (Biosearch Technologies) and Hoechst stain (1:2000 ...
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bioRxiv - Immunology 2023Quote: Antigen-specific antibodies were captured from immunized mice sera in EIA High Binding surface chemistry 96-well plates (Costar) coated with NP26-BSA (Biosearch technologies) and incubated with dilutions of sera ...
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bioRxiv - Immunology 2021Quote: ... n2019-nCoV (Biosearch technologies, KIT-NCOV-PP1-1000). For subgenomic N-gene quantification ...
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bioRxiv - Immunology 2024Quote: ... with a 1:1 mixture of 50 µg NP17-OVA ((Biosearch Technologies/BioCat) in PBS and Imject Alum (Thermo Fisher)) ...
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bioRxiv - Genomics 2023Quote: ... 10 x 200 µL aliquots of extraction buffer (Dissociation Buffer, 1% Kollidon VA64, 1% Triton X100, 0.01% BSA, 666 units/mL RNase-inhibitor (Biosearch technologies, 30281-1)) were dispensed onto the puck for a total volume of 2 mL ...
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bioRxiv - Genetics 2024Quote: The method described here depends on the activity of EZ-Tn5 Transposase (EZ-Tn5 is commonly sold in kits for specific purposes. The enzyme by itself, not part of a kit can be purchased from LGC Biosearch Technologies, Petaluma, CA). This enzyme only requires a 19-nucleotide inverted repeat at each end of a linear blunt-end DNA fragment to catalyze the random insertion of the DNA fragment into a target DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... coli (BioSearch Technologies 60345-1), and overnight cultures were grown in LB supplemented with 25 µg/mL kanamycin ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was polyadenylated with Poly(A) Polymerase Tailing Kit (BIOSEARCH TECHNOLOGIES). Then the polyadenylated product was reversely transcribed into cDNA using a poly(T ...
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bioRxiv - Cancer Biology 2023Quote: ... or 100 ug of the highly substituted hapten NP (NP16 to NP32) conjugated to the carrier protein ovalbumin (OVA) (Biosearch Technologies; N-5051) absorbed to Imject Alum Adjuvant (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris RNA-FISH probes labelled with Quasar 570 Dye for NEAT1_2 (SMF-2037-1) (1:100, Biosearch Technologies) were used according to the instructions provided ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... labelled with FAM dye (1:100, Biosearch Technologies) were used and the procedure was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification kit (LGC Biosearch Technologies) and quantified by NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the MasterPure Gram-Positive DNA purification Kit (Biosearch Technologies) with a modified protocol that included a bead-beating step [53] ...
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bioRxiv - Genomics 2022Quote: ... Cells were then incubated in 70% ethanol at 4°C for at least 1 h and then washed with 1 ml of Wash Buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Nitrophenyl-haptenated ovalbumin (NP-OVA, 25:1 ratio) or NP-MOG (25:1 ratio) were generated in house using NP-OSu (Biosearch Technologies Inc.) and either Ovalbumin protein (Sigma ...
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bioRxiv - Immunology 2020Quote: ... NP-PE (N-5070-1, Biosearch Technologies, Novato CA), CD138-BV650 (281-2 ...
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bioRxiv - Genomics 2023Quote: ... 50 µl of RNase-inhibitor (Biosearch technologies, 30281-1)) which was added to nuclei suspension ...
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bioRxiv - Cell Biology 2024Quote: GAPDH smFISH probe (Biosearch Technologies Inc.: SMF-2026-1). All other probes were generated using Stellaris probe design tool.
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Microbiology 2019Quote: ... genomic RNA was detected with a 5’ 6-carboxyfluorescein (FAM) and 3’ black hole quencher 1 (BHQ-1) labeled probe targeting nsp2 (Biosearch Technologies, Petaluma, CA), and RNA copy number was calculated by reference to an RNA standard derived from the MHV A fragment ...
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bioRxiv - Molecular Biology 2019Quote: ... 1-3 μL of HIV-1 RNA probe diluted in hybridization buffer (Stellaris RNA FISH Hybridization Buffer (Biosearch Tech. Cat# SMF-HB1-10)) and 10% formamide were spotted on coverslips and incubate overnight in humid conditions at 37C ...
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bioRxiv - Molecular Biology 2023Quote: Assay mixes were prepared in 16 μL volumes for each unique crRNA target consisting of 1 μL of 50 U/μL NxGen T7 Polymerase (Biosearch Technologies 30223-1), 2 μL of 800 nM LwaCas13a (Genscript) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies, Middleton, WI, USA) and quantified on the QIAxpert® spectrophotometer (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted from EVs using the MasterPure Complete DNA and RNA Purification Kit (Biosearch Technologies). For comparison purposes ...