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Citations for Biosearch Technologies :
1 - 50 of 60 citations for Mouse Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 96-well ELISA plates (42592, Costar) were coated with NP23-BSA (Biosearch Technologies) in PBS overnight and washed ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Immunology 2023Quote: ... The titers of NP-specific antibody isotypes in sera were measured by ELISA using plates coated with NP(7)- BSA or NP(20)-BSA (Biosearch Technologies) as described previously (63).
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bioRxiv - Genomics 2023Quote: ... Next the samples were transformed into electrocompetent cells (Biosearch Technologies, 60242-2). One 0.1cm cuvette (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were washed with 2 mL of wash buffer A (LGC Biosearch Technologies) supplemented with 10% deionized formamide (Agilent ...
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bioRxiv - Cancer Biology 2019Quote: ... Coverslips were then washed with 2 mL of Wash buffer A (LGC Biosearch Technologies) at RT for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: The lysate was incubated with 2 U of RNase I (LGC Biosearch Technologies, low), 10 U of RNase I (high) ...
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bioRxiv - Genomics 2019Quote: ... We then washed the coverslips with 2 mL of Wash buffer A (LGC Biosearch Technologies) at room temperature for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 smFISH probes were designed using Stellaris smFISH probe designer (Biosearch Technologies) available online at http://www.biosearchtech.com/stellaris-designer ...
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bioRxiv - Microbiology 2021Quote: ... A final wash step was performed using Wash buffer B (Biosearch Technologies Cat# SMF-WB1-2) for 5 mins before mounting coverslips with ProLong Gold Anti-Fade reagent (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: 2-4 months old mice were immunized intraperitoneally with 100 μg of NP18-OVA (Biosearch technologies) in Imject Alum adjuvant (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... n2019-nCoV (Biosearch technologies, KIT-NCOV-PP1-1000). For subgenomic N-gene quantification ...
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bioRxiv - Cell Biology 2019Quote: ... ChIRP was performed using biotinylated probes designed against mouse lnc-Nr2f1 using the ChIRP probes designer (Biosearch Technologies). Independent even and odd probe pools were used to ensure lncRNA-specific retrieval as protocols previously described3 ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIRP was performed using biotinylated oligo probes designed against mouse MaTAR25 using the ChIRP probe designer (Biosearch Technologies). Independent even and odd probe pools were used to ensure lncRNA-specific retrieval (refer to Table S1 for odd and even sequences targeting MaTAR25 ...
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bioRxiv - Genetics 2022Quote: ... slow-2 and pgl-1 using the Stellaris RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Developmental Biology 2024Quote: ... and kl-2 exons were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc.) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Genetics 2024Quote: The method described here depends on the activity of EZ-Tn5 Transposase (EZ-Tn5 is commonly sold in kits for specific purposes. The enzyme by itself, not part of a kit can be purchased from LGC Biosearch Technologies, Petaluma, CA). This enzyme only requires a 19-nucleotide inverted repeat at each end of a linear blunt-end DNA fragment to catalyze the random insertion of the DNA fragment into a target DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... All SSOs used are RNA oligonucleotides with 2’-O-methyl modifications and phosphorothioate backbones (LGC Biosearch Technologies; Risskov, Denmark) (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were washed with PBS and DNA extracted using QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and recovering for 90 minutes at 30 °C in 1 mL of Endura recovery media (Biosearch Technologies 60242-2). Cultures were then grown for 16 hours in 50 mL of 2xYT media with 100 µg/mL of carbenicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos underwent heat shock and were hybridized for 2 h at 38.5 °C in a humidity chamber with XIST Quasar 570 (125 nM; SMF-2038-1; BioSearch Technologies) in a hybridization buffer ...
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bioRxiv - Microbiology 2023Quote: ... RNA was polyadenylated with Poly(A) Polymerase Tailing Kit (BIOSEARCH TECHNOLOGIES). Then the polyadenylated product was reversely transcribed into cDNA using a poly(T ...
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bioRxiv - Cancer Biology 2023Quote: ... or 100 ug of the highly substituted hapten NP (NP16 to NP32) conjugated to the carrier protein ovalbumin (OVA) (Biosearch Technologies; N-5051) absorbed to Imject Alum Adjuvant (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... smFISH probes consisting of 20 nt oligonucleotides with 2 nt spacing were designed with Stellaris Probe Designer and synthesized by Biosearch Technologies. Probe sets complementary to cycB (CG3510 ...
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bioRxiv - Microbiology 2021Quote: ... The abundance of SARS-CoV-2 genetic material was subsequently determined by quantitative PCR using a Taqman probe and primers (Biosearch Technologies). The primers and probe sequences for the were as recommended by BEI resources for compatability with synthetic RNA standards (# NR-52358).
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bioRxiv - Genetics 2024Quote: ... arrested cells were released into rich media containing 200 mM hydroxyurea and 130 μM Edu (5-Ethynyl-2’-deoxyuridine) (Biosearch Technologies). Cells were harvested in G1 and after 60 minutes release into HU ...
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bioRxiv - Genomics 2024Quote: ... The assembled plasmid pool was purified and concentrated with AMPure XP SPRI Reagent and used for transformation of Electrocompetent Endura Cells (#60242-2, Biosearch Technologies) following manufacturer’s instructions and a previously published protocol47 ...
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bioRxiv - Genomics 2024Quote: ... Purified ligations were electrotransformed by combining 5 µL of ligation and 25 µL of Endura electrocompetent cells (Biosearch Technologies 60242-2) in a 0.1 cm Gene Pulser cuvette (Biorad 1652083 ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 100 μl of hybridization buffer (100 mg/ml dextran sulfate, 10–15% formamide, 2× SSC) containing 125 nM Stellaris probes (LGC Biosearch Technologies) for 48 h.
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed with Stellaris Wash Buffer A solution (2 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies SMF-WA1-60), 7 mL Nuclease-free water ...
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bioRxiv - Immunology 2024Quote: ... were coated with 25 µl of 10 µg/ml NP-BSA in PBS (N-5050XL-10-BS with loading ratio of 2 or N-5050H-10-BS with loading ratio of 36, both Biosearch Technologies/BioCat) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were re-fixed with 4% formaldehyde for 10 min and then hybridized with Stellaris RNA FISH probe for mouse Xist with Quasar 570 dye (BioSearch Tech, SMF-3011-1). First ...
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bioRxiv - Genetics 2022Quote: ... and stored at 4°C for several days. A lag-2 RNA probe set (Barkoulas et al. 2013) coupled to AF594 (Custom Stellaris Fish Probes, Biosearch Tech, Teddington UK) was resuspended in RNase-free TE buffer (pH8 ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was isolated using Master Pure Complete DNA&RNA purification kit (Biosearch technologies) according to the manufacturer’s instructions except that scale was increased 2 times ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of the TdT-labeled probes was added to 98 μL of Stellaris RNA FISH Hybridization Buffer (Biosearch Technologies Cat# SMF-HB1-10); 100 μL of this mixture was dotted onto the Parafilm in the humidifying chamber ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted by using MasterPure Yeast DNA purification kit (Biosearch Technologies, cat # MPY80200). The DNA samples were quantified and diluted ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification kit (LGC Biosearch Technologies) and quantified by NanoDrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the MasterPure Gram-Positive DNA purification Kit (Biosearch Technologies) with a modified protocol that included a bead-beating step [53] ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested 2-3 days post-electroporation and genomic DNA (gDNA) was harvested using QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK, cat.: QE09050). To quantify knock-in alleles via ddPCR ...
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted with MasterPure™ Complete DNA & RNA Purification Kit (MC89010, LGC Biosearch Technologies). Isolates were grown overnight on blood agar (PP0120-9090 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies, Middleton, WI, USA) and quantified on the QIAxpert® spectrophotometer (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted from EVs using the MasterPure Complete DNA and RNA Purification Kit (Biosearch Technologies). For comparison purposes ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenised material was then treated following the Sbeadex® plant mini kit protocol (LGC Biosearch Technologies, Beverley, MA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Fmr1 mutants was reserve transcribed to cDNA using MMLV High-Performance Reverse Transcriptase Kit (Biosearch Technologies #RT80125K) and oligo(dT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was prepared from 0.5 ml of culture with the MasterPure Yeast DNA Purification kit (Biosearch Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Pure lactobacilli isolates had their DNA extracted using MasterPure™ Gram Positive Purification Kit (LGC Biosearch Technologies, Hoddesdon, UK). All DNA extraction quantifications were performed with Qubit Fluorometer (Thermo Fischer Scientific ...