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Citations for Biosearch Technologies :
101 - 150 of 240 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... we used Stellaris FISH probes targeting Renilla luciferase and labelled with the Quasar 570 dye (Biosearch Technologies). Cells were plated on glass coverslips (High Precision ...
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bioRxiv - Cell Biology 2021Quote: Single molecule mRNA fluorescence in situ hybridization (smFISH) was performed with Stellaris FISH probes (LCG Biosearch Technologies) as per the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... parisii 18S rRNA-specific microB FISH probe (ctctcggcactccttcctg) conjugated to Cal Fluor Red 610 (LGC Biosearch Technologies) was used (27) ...
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bioRxiv - Genetics 2022Quote: ... slow-2 and pgl-1 using the Stellaris RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-molecule RNA FISH was performed according to the protocol for adherent cells provided by Biosearch technologies. Briefly ...
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bioRxiv - Genomics 2023Quote: ... Meronts and sporoplasms were visualized using FISH probes conjugated to CAL Fluor Red 610 (LGC Biosearch Technologies). N ...
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bioRxiv - Genetics 2022Quote: ... Probes against Mst77F and Mst77Y were designed using the Stellaris1 RNA FISH Probe Designer (Biosearch Technologies, Inc.) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then designed RNA-probes for our target sequences using the Stellaris RNA FISH platform (Biosearch Technologies) with the custom probe design service following their recommendations for probe design.
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bioRxiv - Genomics 2023Quote: ... Slides were hybridized with GREB1 or NRIP1 Stellaris FISH Probe sets labeled with Quasar 670 (Biosearch Technologies) following the manufacturer’s instructions (www.biosearchtech.com/stellarisprotocols) ...
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bioRxiv - Developmental Biology 2024Quote: ... and kl-2 exons were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc.) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris RNA-FISH probes labelled with Quasar 570 Dye for NEAT1_2 (SMF-2037-1) (1:100, Biosearch Technologies) were used according to the instructions provided ...
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bioRxiv - Biophysics 2020Quote: ... in a total volume of 200 μl of the hybridization buffer (Stellaris RNA FISH hybridization buffer, Biosearch Technologies, supplemented with 10% v/v Hi-Di formamide) ...
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bioRxiv - Neuroscience 2020Quote: FISH probes were designed based on transcript sequences using the online Stellaris Designer and purchased from Biosearch Technologies. Probe sequences for AstC and vGlut were previously reported (Long et al, ...
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bioRxiv - Biochemistry 2021Quote: ... in a total volume of 200 μl of the hybridization buffer (Stellaris RNA FISH hybridization buffer, Biosearch Technologies, supplemented with 10% v/v Hi-Di formamide) ...
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bioRxiv - Genomics 2021Quote: ... Probe libraries were designed using the Stellaris FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA, see Table S7) and covalently coupled to Cy5 (Lyubimova et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Probe set against nos exons was designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc.) available online at https://www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with 10% v/v formamide in Stellaris® RNA FISH wash buffer A (Biosearch Technologies) and incubated in 1 mL of DAPI staining solution (5 ng/mL DAPI in wash buffer A with 10% v/v formamide ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL Hybridization Buffer (90 μL Stellaris® RNA FISH Hybridization Buffer (Cat# SMF-HB1-10, LGC Biosearch Technologies), 10 μL deionized formamide ...
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bioRxiv - Genomics 2020Quote: ... Coverslips were washed twice for 30 min at 37°C with Stellaris RNA FISH Wash Buffer A (Biosearch Technologies), with 0.2 mg/ml Dapi being added to the second wash ...
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bioRxiv - Biochemistry 2019Quote: NORAD-Quasar670 smFISH probes were custom made using Stellaris® RNA FISH probe Designer (Biosearch Technologies, Inc., Petaluma, CA). The probe sequences are listed in Table S2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed/equilibrated in buffer A (Stellaris RNA FISH Wash Buffer A (Biosearch Tech. Cat# SMF-WA1-60) with 10% formamide in water ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were hybridized with Stellaris RNA FISH Probe sets labeled with CalFluor 610 or Quasar 670 (Biosearch Technologies, Inc.), following the manufacturer’s instructions available online at www.biosearchtech.com/stellarisprotocols ...
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bioRxiv - Neuroscience 2021Quote: ... The cultured primary neurons were hybridized with the ADRAM Stellaris FISH Probe set labeled with TAMRA (Biosearch Technologies, Inc.), following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... and eIF4E-5 mRNA were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Novato, USA) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Molecular Biology 2021Quote: ... and samples were subsequently incubated with Stellaris® RNA FISH Wash Buffer B (Biosearch Technologies; Cat #: SMF-WB1-20) as prepared through manufacturers protocol for 5 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: Experiments were performed with slight modifications to the Stellaris protocol for simultaneous FISH-immunofluorescence in adherent cells (Biosearch Technologies)35–37 ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then washed twice with Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) with the second wash left to incubate for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 8 FISH probes (IDT) (Table S3) marked with FAM were designed using the Stellaris probe designer software (Biosearch Technologies). Cells were grown either on Falcon® 8-well Culture Slide (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... smFISH was performed using SunTag-V4 probes (59) labeled with Quasar 670 dye (Stellaris RNA FISH probes, Biosearch Technologies), as described above ...
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bioRxiv - Neuroscience 2024Quote: ... see Table S2 for probe sequences) were designed using the Stellaris RNA-FISH Probe Designer 2.0 (LGC Biosearch Technologies) with non-specificity masking level 5 with the target sequence between the entire ins-6 open reading frame along with the 5’ and 3’ untranslated regions ...
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bioRxiv - Systems Biology 2023Quote: ... permeabilised in ethanol and hybridized with a recB specific set of TAMRA-labelled RNA FISH probes (LGC Biosearch Technologies). Probe sequences are listed in Table 6 ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated with Quasar 670-conjugated FISH probes that hybridize to Orsay virus RNA1 and RNA2 (Biosearch Technologies). Following a 6-8 h incubation at 47 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and NFKBIA probes were generated for smRNA-FISH using the Stellaris Probe Designer (https://www.biosearchtech.com/support/tools/design-software/stellaris-probe-designer) (Biosearch Technologies, UK) according to the protocols of Biosearch Technologies ...
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bioRxiv - Neuroscience 2019Quote: The fluorescence in situ hybridization (FISH) probe libraries were designed based on transcript sequences and were purchased from Biosearch Technologies. The FISH protocol and dye labeling procedures were described previously (Long et al. ...
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bioRxiv - Systems Biology 2020Quote: ... and hybridized with Stellaris FISH probes labeled with Quasar 570 or Quasar 670 at 125nM (Biosearch Technologies, Supplementary Table 1) overnight at 30°C in Hybridization Buffer (containing 20% Formamide (Ambion AM9342) ...
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bioRxiv - Microbiology 2019Quote: ... and GFP RNA detected by incubation using 0.125 µM Cy5-labeled probes in Stellaris RNA FISH hybridization buffer (Biosearch Technologies) for 18 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 10% deionized formamide for 5 min, and incubated with hybridization mix (0.1 μM RNA FISH, 10% deionized formamide, in Hybridization Buffer (Biosearch Technologies)) overnight at 37°C in the dark ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with Wash buffer A (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies, Inc., SMF-WA1-60), 10% Deionized Formamide (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Hybridization with CAL Fluor Red 610-conjugated anti-PEX14 mRNA FISH probes tiling the length of the PEX14 ORF (0.125 μM, Biosearch Technologies) was carried out overnight at 30°C in hybridization buffer (10% formamide ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 110 µL Hybridization Buffer [99 µL Stellaris RNA FISH Hybridization Buffer (LGC Biosearch Technologies, SMF-HB1-10), 11 µL deionized formamide] containing 1.1 µL 12.5 µM vgRNA FISH probes for 4 hours at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the cells were fixed with 3.7% formaldehyde in PBS for 10 minutes again and subjected to FISH staining using buffers from Biosearch Technologies as previously described [33] ...
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bioRxiv - Cell Biology 2020Quote: ... Custom Stellaris FISH probes were designed to recognize NL4-3 HIV-1 gag-pol reading frame nucleotides 386-4456 using the Stellaris RNA FISH Probe Designer 4.1 (Biosearch Technologies, Inc.) available online ...
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bioRxiv - Evolutionary Biology 2020Quote: ... respectively and a commercially available library of 48 probes was used to detect HOXA5 (cat # VSMF-2538-5) (Stellaris RNA FISH probes, Biosearch Technologies). Hybridization conditions and imaging were as previously described (Itzkovitz et al ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μL Wash Buffer A (40 μL Stellaris® RNA FISH Wash Buffer A (Cat# SMF-WA1-60, LGC Biosearch Technologies), 20 μL deionized formamide ...
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bioRxiv - Neuroscience 2021Quote: ... Primary motor neurons were hybridized with the indicated Stellaris FISH Probe set labeled with either Quasar-570 or 670 (Biosearch Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated overnight in the dark at 46 °C with 100 μl of hybridization buffer containing 5 ng/μl of the MicroB FISH probe conjugated to Cal Fluor 610 (LGC Biosearch Technologies). Samples were then washed in 1ml of wash buffer (Hybridization buffer + 5 mM EDTA) ...
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bioRxiv - Cancer Biology 2020Quote: ... A set of Stellaris RNA FISH Probes labeled with Cy5 dye specific to Hmrhl targeted transcript was purchased from Biosearch Technologies. Briefly ...
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bioRxiv - Microbiology 2021Quote: ... and incubated overnight at 46°C in 100 μl hybridization buffer containing 5-10 ng/μl of FISH probe conjugated to a Cal Fluor 610 dye (LGC Biosearch Technologies). 5 ng/μl MicroB (ctctcggcactccttcctg)27 was used to detect N ...
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bioRxiv - Microbiology 2019Quote: ... in a total volume of 200 µl of the hybridization buffer (Stellaris RNA FISH hybridization buffer, SMF-HB1-10, Biosearch Technologies, supplemented with 10% v/v of deionized formamide) ...
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was then removed and 1 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) with 10% deionized formamide for 5 min at room temperature ...