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Citations for Biosearch Technologies :
1 - 50 of 156 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... samples were incubated with Quasar 670-conjugated FISH probes that hybridize to Orsay virus RNA1 and RNA2 (Biosearch Technologies). Following a 6-8 h incubation at 47 °C ...
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bioRxiv - Immunology 2024Quote: ... worms were incubated at 47 °C overnight with fluorescein (FAM)- or Quasar 670-conjugated FISH probes that target Orsay virus RNA1 and RNA2 (Biosearch Technologies). Infection rate was assessed using a Zeiss AxioImager M1 compound microscope ...
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bioRxiv - Neuroscience 2023Quote: ... After electroporation transformation with Endura Electrocompetent Cells (60242-1, Biosearch Technologies, USA), the cells were plated into Nunc™ Square BioAssay Dishes (Catalog number ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was then resuspended with 100 µL per 1 million cells of QuickExtract (Pt#SS000035-D1 Biosearch Technologies). The hiPSCs and PBMCs were then run in a thermocycler for one round of three consecutive cycles ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaned assembly reactions were electroporated into Endura Electrocompetent cells (Biosearch Technologies 60242-1) according to manufacturer’s instructions and plated on LB-Lennox 250 mm x 250 mm square bioassay dishes supplemented with 75 µg/mL carbenicillin ...
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bioRxiv - Genomics 2022Quote: ... Cells were then washed with 1 ml of Wash Buffer B (LGC Biosearch Technologies) at RT for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then resuspended in 1 mL wash buffer B (Biosearch Technologies, SMF-WB1-20), incubated for 2-5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with 1 ml of Wash buffer A (Biosearch Technologies, SMF-WA1-60). To probe for S4 mRNA ...
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bioRxiv - Genetics 2023Quote: ... and then resuspended with 100 µL per 1 million cells of QuickExtract (Pt#SS000035-D1 Biosearch Technologies). The hiPSCs were then run in a thermocycler for three consecutive cycles ...
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bioRxiv - Genomics 2023Quote: ... 1 mL of recovery media (Biosearch Technologies, 80026-1) was transferred to each culture tube in advance ...
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bioRxiv - Immunology 2024Quote: ... with a 1:1 mixture of 50 µg NP17-OVA ((Biosearch Technologies/BioCat) in PBS and Imject Alum (Thermo Fisher)) ...
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bioRxiv - Genomics 2023Quote: ... 10 x 200 µL aliquots of extraction buffer (Dissociation Buffer, 1% Kollidon VA64, 1% Triton X100, 0.01% BSA, 666 units/mL RNase-inhibitor (Biosearch technologies, 30281-1)) were dispensed onto the puck for a total volume of 2 mL ...
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bioRxiv - Microbiology 2023Quote: ... ClearColiÒ BL21(DE3) cells (Biosearch Technologies) were cultured per manufacturer instructions.
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bioRxiv - Biochemistry 2023Quote: ... coli C43(DE3) cells (Biosearch Technologies)72 ...
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bioRxiv - Genetics 2024Quote: ... cloni EXPRESS electrocompetent cells (Biosearch Technologies) along with a donor plasmid and an inactive Kanamycin resistance gene ...
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bioRxiv - Genetics 2024Quote: ... coli EXPRESS electrocompetent cells (Biosearch Technologies) with a target plasmid encoding the T5 target sequence and a T7-inducible IS621 recombinase ...
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bioRxiv - Molecular Biology 2022Quote: ... coli (BioSearch Technologies 60345-1), and overnight cultures were grown in LB supplemented with 25 µg/mL kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... coli EPI300 cells (LGC Biosearch Technologies, UK). Sequences of all obtained plasmids were confirmed using Sanger sequencing and are available form authors upon request.
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bioRxiv - Cancer Biology 2023Quote: ... Endura Electrocompetent cells (LGC Biosearch Technologies, #602422) were transformed with the DDR-CRISPR library by electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... or 100 ug of the highly substituted hapten NP (NP16 to NP32) conjugated to the carrier protein ovalbumin (OVA) (Biosearch Technologies; N-5051) absorbed to Imject Alum Adjuvant (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... NP-specific B cells or SA-specific B cells were detected with BCR specific binding with NP-PE (Biosearch Technologies) or SA-PE (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris RNA-FISH probes labelled with Quasar 570 Dye for NEAT1_2 (SMF-2037-1) (1:100, Biosearch Technologies) were used according to the instructions provided ...
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bioRxiv - Immunology 2020Quote: ... percentage of NP-binding B1-8hi cell were determined by staining a fraction of cells with 5 mg/mL NP(19)-PE (Biosearch Technologies) and analyzing by flow cytometry.
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was extracted from 250,000 cells for each clonal cell line in 25 uL QuickExtractTM DNA Extraction Solution (BioSearch Technologies #QE09050) by thermocycling at 65°C for 6 minutes followed by 98°C for 2 minutes ...
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bioRxiv - Genetics 2024Quote: ... and electroporated in Endura DUO electrocompetent cells (Biosearch Technologies). Hundreds of thousands of colonies were isolated for sufficient coverage of the oligo library ...
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bioRxiv - Microbiology 2024Quote: ... Cells were fixed again with 3.7 % formaldehyde (Biosearch technologies) diluted in PBS 1X for10 min at room temperature and incubated over night at room temperature with the SINV genome specific LGC Biosearch Technologies’ Stellaris® RNA FISH Probe diluted in RNA FISH hybridization buffer (Stellaris ...
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bioRxiv - Genomics 2022Quote: ... labelled with FAM dye (1:100, Biosearch Technologies) were used and the procedure was carried out according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Cells were then incubated in 70% ethanol at 4°C for at least 1 h and then washed with 1 ml of Wash Buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Nitrophenyl-haptenated ovalbumin (NP-OVA, 25:1 ratio) or NP-MOG (25:1 ratio) were generated in house using NP-OSu (Biosearch Technologies Inc.) and either Ovalbumin protein (Sigma ...
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bioRxiv - Immunology 2020Quote: ... NP-PE (N-5070-1, Biosearch Technologies, Novato CA), CD138-BV650 (281-2 ...
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bioRxiv - Genomics 2023Quote: ... 50 µl of RNase-inhibitor (Biosearch technologies, 30281-1)) which was added to nuclei suspension ...
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bioRxiv - Cell Biology 2024Quote: GAPDH smFISH probe (Biosearch Technologies Inc.: SMF-2026-1). All other probes were generated using Stellaris probe design tool.
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bioRxiv - Microbiology 2019Quote: ... genomic RNA was detected with a 5’ 6-carboxyfluorescein (FAM) and 3’ black hole quencher 1 (BHQ-1) labeled probe targeting nsp2 (Biosearch Technologies, Petaluma, CA), and RNA copy number was calculated by reference to an RNA standard derived from the MHV A fragment ...
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bioRxiv - Molecular Biology 2019Quote: ... 1-3 μL of HIV-1 RNA probe diluted in hybridization buffer (Stellaris RNA FISH Hybridization Buffer (Biosearch Tech. Cat# SMF-HB1-10)) and 10% formamide were spotted on coverslips and incubate overnight in humid conditions at 37C ...
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bioRxiv - Molecular Biology 2023Quote: Assay mixes were prepared in 16 μL volumes for each unique crRNA target consisting of 1 μL of 50 U/μL NxGen T7 Polymerase (Biosearch Technologies 30223-1), 2 μL of 800 nM LwaCas13a (Genscript) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were hybridized with 80 μL hybridization buffer (LGC Biosearch Technologies) containing properly diluted smRNA FISH probes (1:100 ...
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bioRxiv - Genomics 2019Quote: ... we hybridized cells with 80 μL hybridization buffer (LGC Biosearch Technologies) containing Tug1 probes (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed using wash buffer A (LGC Biosearch Technologies) for 30 min at 37□°C ...
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bioRxiv - Microbiology 2024Quote: ... then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat. # 60242). Five hundred thousand colonies were pooled ...
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bioRxiv - Developmental Biology 2022Quote: ... and a Stellaris FISH probe (Biosearch Technologies, SMF-2038-1), which was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... we used a Stellaris probe (Biosearch Technologies, SMF-2038-1) to detect XIST RNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then washed in 500 μL Wash Buffer B (Biosearch Technologies), and resuspended in 50 μL freshly filtered 5× saline-sodium citrate (SSC ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were hybridized with 80 μL of hybridization buffer (LGC Biosearch Technologies) supplemented with 10% deionized formamide (Agilent ...
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bioRxiv - Cell Biology 2019Quote: ... Next cells were incubated with oligo43-Quasar 670 probes (LGC Biosearch Technologies) for 4h at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... cells were incubated with wash B buffer (Biosearch Technologies, SMF-WB1-20) at RT for 5 min ...
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bioRxiv - Genomics 2023Quote: ... Next the samples were transformed into electrocompetent cells (Biosearch Technologies, 60242-2). One 0.1cm cuvette (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Assembled plasmid was transformed by elctroporation into Endura competent cells (Biosearch technologies), followed by DNA prepping of the libraries.
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bioRxiv - Genetics 2023Quote: ... The assembled products were electroporated into Endura electrocompetent cells (Biosearch Technologies 60242), and approximately 4,000 independent bacterial colonies were cultured for each library ...
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bioRxiv - Cell Biology 2023Quote: ... Ligations were transformed into Endura Electrocompetent Cells (LCG Biosearch Technologies, Middlesex, UK) using a Gene Pulser II (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in QuickExtract™ DNA Extraction Soln 1.0 (BIOSEARCH Technologies) for 15 min at 68 °C and 15 min at 98 °C ...