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Citations for Biosearch Technologies :
101 - 150 of 156 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Staining was performed with 1 µM Cal Fluor 610 conjugated pals-5 smFISH probes (Biosearch Technologies) in smFISH hybridization buffer (10% formamide ...
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bioRxiv - Molecular Biology 2020Quote: ... then in 1 mL Stellaris RNA FISH Wash Buffer B (Biosearch Technologies Cat# SMF-WB1-20) with Hoescht stain at 1:10,000 dilution for 5 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was supplemented with 1 U/µl of an RNase inhibitor (302811, LGC Biosearch Technologies).
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bioRxiv - Microbiology 2024Quote: ... 5 μL Baseline-ZERO™ DNAse enzyme (LGC Biosearch Technologies, Pt# E0110-D1, 1 U/μL), 10 μL 10X Baseline-ZERO™ DNase Reaction Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were challenged with 100 ng/mL TNP-BSA (T-5050-100, LGC Biosearch Technologies, Novato, CA) for 2 additional hours before the cells were collected for further analysis.
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with 10% v/v formamide in Stellaris® RNA FISH wash buffer A (Biosearch Technologies) and incubated in 1 mL of DAPI staining solution (5 ng/mL DAPI in wash buffer A with 10% v/v formamide ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the genomic DNA of the cells (5U OD) was extracted using MasterPure Yeast DNA Purification Kit (Biosearch Technologies) before and after two cycles of PCA selection (S2 and MTX2) ...
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bioRxiv - Genetics 2022Quote: ... slow-2 and pgl-1 using the Stellaris RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed/equilibrated in buffer A (Stellaris RNA FISH Wash Buffer A (Biosearch Tech. Cat# SMF-WA1-60) with 10% formamide in water ...
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bioRxiv - Microbiology 2022Quote: Experiments were performed with slight modifications to the Stellaris protocol for simultaneous FISH-immunofluorescence in adherent cells (Biosearch Technologies)35–37 ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were washed with PBS and DNA extracted using QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ∼5x107 cells were collected and total RNA was extracted using a MasterPure Yeast RNA purification kit (Biosearch Technologies, MPY03100) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... NIC203 and EG6180 embryos were hybridised with slow-1 probes labeled with Quasar® 570 (Biosearch Technologies, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with Wash buffer A (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies, Inc., SMF-WA1-60), 10% Deionized Formamide (EMD Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was aspirated and the cells were washed once with 0.5 ml Stellaris RNA wash buffer A (Biosearch technologies). The cells were then incubated with 100 μl Stellaris hybridization buffer (Biosearch Technologies ...
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bioRxiv - Genomics 2022Quote: smRNA-FISH experiments were performed according to the Stellaris® RNA FISH protocol for adherent cells provided by Biosearch Technologies. Specifically ...
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bioRxiv - Microbiology 2023Quote: ... Single molecule FISH (smFISH) was performed using Stellaris™ RNA FISH protocol in Adherent Cells (Biosearch Technologies, Novato, CA, USA). After fixation ...
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bioRxiv - Genomics 2022Quote: ... and 2.5 µL of the reaction was transformed into 25 µL of Lucigen Endura ElectroCompetent Cells (Biosearch Technologies, Hoddesdon, UK). Before culture for maxiprep ...
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bioRxiv - Neuroscience 2021Quote: ... single cell samples were prepared for 3’ mRNA sequence determinations using the Scipio bioscience protocol (to be published) with barcoding beads (NxSeq Single-cell RNA-seq Beads, LGC Biosearch Technologies). For beads that captured mRNA molecules ...
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bioRxiv - Immunology 2020Quote: T-cell dependent immune responses were induced by intraperitoneally injecting mice with NP-CGG (N-5055B-5, Biosearch Technologies, Novato CA), as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... Hybridization step occurred in a parafilm-sealed humidity chamber with cells incubated in 125 nM RNA FISH probes (Supplementary Table 3)-containing Stellaris Hybridization Buffer (Biosearch Tech) for 16 hrs at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... the glass coverslips were transferred to a fresh 6-well plate with the cell side up and incubated with Wash Buffer A (Biosearch Technologies) and Hoechst stain (1:2000 ...
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bioRxiv - Genetics 2024Quote: ... arrested cells were released into rich media containing 200 mM hydroxyurea and 130 μM Edu (5-Ethynyl-2’-deoxyuridine) (Biosearch Technologies). Cells were harvested in G1 and after 60 minutes release into HU ...
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bioRxiv - Genomics 2024Quote: ... The assembled plasmid pool was purified and concentrated with AMPure XP SPRI Reagent and used for transformation of Electrocompetent Endura Cells (#60242-2, Biosearch Technologies) following manufacturer’s instructions and a previously published protocol47 ...
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bioRxiv - Genomics 2024Quote: ... Purified ligations were electrotransformed by combining 5 µL of ligation and 25 µL of Endura electrocompetent cells (Biosearch Technologies 60242-2) in a 0.1 cm Gene Pulser cuvette (Biorad 1652083 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5X sample premixes were created with 2.4 μL of 10X T7 Buffer (Biosearch Technologies F88905-1, Hoddesdon, United Kingdom), 3.2 μL of 7.5X sample buffer ...
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bioRxiv - Genomics 2024Quote: ... and recovering for 90 minutes at 30 °C in 1 mL of Endura recovery media (Biosearch Technologies 60242-2). Cultures were then grown for 16 hours in 50 mL of 2xYT media with 100 µg/mL of carbenicillin ...
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bioRxiv - Molecular Biology 2023Quote: ... 56 Subsequent IVT reaction producing 5′-triphosphorylated RNAs was performed with NxGen T7 RNA Polymerase (Biosearch Technologies #30223-1). For segment 8 of IAV MEGAscript T7 Transcription Kit (Thermo #AM1333 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were further washed in Wash Buffer I (20% Stellaris RNA FISH Wash Buffer A (Biosearch Technologies, Inc., SMF-WA1-60), 10% Deionized Formamide (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... The coverslips with cells were immersed in 100µl hybridization buffer (90µl Stellaris RNA-FISH Hybridization Buffer (Biosearch Technologies, Cat. SMF-HB1-10) and 10µl deionized formamide (Millipore Sigma ...
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bioRxiv - Genomics 2022Quote: ... The cell suspension was further incubated with biotinylated 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP)15-BSA (Biosearch Technologies, N-1027-5) for 1.5h ...
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bioRxiv - Systems Biology 2023Quote: ... each cover glass was set on a droplet (cells facing down) consisting of 45 μL hybridization buffer (Biosearch Technologies, SMF-HB1-10) and 1 μL of the duplex smiFISH probes (MS2-transcript-binding probe mix + FLAP-Y-binding region annealed to FLAP-Y-Cy5 ...
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bioRxiv - Cell Biology 2023Quote: ... 300 uL of cells were spun down and hybridized overnight at 30 °C with 100 uL of Stellaris® RNA FISH hybridization buffer (Biosearch Technologies) containing 10% v/v formamide and 125 mM smFISH probes ...
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bioRxiv - Systems Biology 2020Quote: ... and hybridized with Stellaris FISH probes labeled with Quasar 570 or Quasar 670 at 125nM (Biosearch Technologies, Supplementary Table 1) overnight at 30°C in Hybridization Buffer (containing 20% Formamide (Ambion AM9342) ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryos underwent heat shock and were hybridized for 2 h at 38.5 °C in a humidity chamber with XIST Quasar 570 (125 nM; SMF-2038-1; BioSearch Technologies) in a hybridization buffer ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Genomics 2021Quote: ... Reactions are recovered from the membrane and 2.5 µl of the reaction are transformed into 25 µl of Lucigen Endura ElectroCompetent Cells (Biosearch Technologies, Hoddesdon, United Kingdom). Transformed bacteria are cultured overnight in a shaker before maxiprep.
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bioRxiv - Cell Biology 2020Quote: ... Custom Stellaris FISH probes were designed to recognize NL4-3 HIV-1 gag-pol reading frame nucleotides 386-4456 using the Stellaris RNA FISH Probe Designer 4.1 (Biosearch Technologies, Inc.) available online ...
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bioRxiv - Cell Biology 2021Quote: ... S4 cDNA was detected using a fluorogenic probe (5′-FAM [fluorescent fluorescein]-AGCGCGCAAGAGGGATGGGA-BHQ [black hole quencher]-1-3′; Biosearch Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... The 70% ethanol was then removed and 1 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies Cat# SMF-WA1-60) with 10% deionized formamide for 5 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The probes for the coronavirus assays (E/IP4) were both labelled with a FAM reporter and black hole quencher 1 (Biosearch Technologies) and the XIPC probe with a Vic reporter and TAMRA quencher (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... at the 5′ end as the reporter fluorophore and with Black Hole Quencher 1 (BHQ1) at the 3′ end as the quencher (Biosearch Technologies). qPCR reactions were performed in a 20-µL volume containing 100 ng genomic DNA template (estimated by UV spectrophotometry ...
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bioRxiv - Molecular Biology 2023Quote: ... After fixation with 4% PFA cells were permeabilized by incubation in 70% EtOH for at least 1 hr at 4°C followed by incubation in Stellaris Wash Buffer A (Biosearch Tech) for 5 min at RT ...
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bioRxiv - Cell Biology 2023Quote: Xist RNA was detected with Stellaris® RNA FISH (Human XIST with Quasar® 570 Dye, Biosearch Technologies, SMF-2038-1) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:6 in nuclease free H2O and 5 µl were used for Kompetitive allele specific PCR (KASP) genotyping (LGC Biosearch Technology) (Supplementary Table 3) ...
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bioRxiv - Genomics 2023Quote: ... Cover glasses were incubated in 70% ethanol at 4°C for at least one hour and then washed with 1 mL of wash buffer A (LGC Biosearch Technologies) at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... permeabilized cells were washed with 200 µL Wash Buffer A [40 µL Stellaris RNA FISH Wash Buffer A (LGC Biosearch Technologies, SMF-WA1-60), 20 µL deionized formamide ...
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bioRxiv - Microbiology 2020Quote: ... Ethanol 70% is discarded and cells washed with 200 μL wash buffer A (10% vol./vol. formamide in 1X Wash Buffer A, Biosearch Technologies Cat# SMF-WA1-60). Cells were incubated with 100 μL Hybridization buffer containing 1,25 μM of RNA-FISH probes in the dark at 37 °C overnight (~16 hours) ...