Labshake search
Citations for Biosearch Technologies :
51 - 80 of 80 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... Genomic DNA was extracted using Lucigen QuickExtract DNA extraction solution (Biosearch Technologies, Inc) according to the manufacturer’s direction ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted using QuickExtract (QE) DNA Extraction Solution (Biosearch Technologies, QE09050). TrypLE Express Enzyme was added to cells ...
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bioRxiv - Genetics 2023Quote: ... A sample was lysed with QuickExtract™ DNA Extraction Solution (Biosearch Technologies QE09050) to assess editing efficiency with the Alt-R® Genome Editing Detection Kit (Integrated DNA Technologies 1075932) ...
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bioRxiv - Microbiology 2024Quote: ... Collected supernatants were immediately RNA extracted using QuickExtract DNA Extraction Solution (Biosearch Technologies) 57 ...
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bioRxiv - Bioengineering 2024Quote: The other plate was resuspended in 50 µL QuickExtract DNA Solution (Biosearch Technologies), vortexed for 15 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was extracted using 50-150 µL QuickExtract DNA Extraction Solution (LGC, Biosearch Technologies), depending on the pellet size ...
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bioRxiv - Biochemistry 2023Quote: ... The worms were dissolved in 10ul of QuickExtract(tm) DNA Extraction Solution (Biosearch Technologies). Final analyses of homozygous or heterozygous lines were performed by PCR reaction using Ready To Use PCR MasterMix (Central European Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... supernatants were immediately diluted 4:1 into QuickExtract DNA Extraction Solution (Biosearch Technologies, #QE09050), homogenized ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was isolated from iPSC clones using QuickExtract DNA Extraction Solution (Biosearch Technologies QE09050), PCR amplification was performed using primers surrounding the mutation site (Supplemental Table 2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the female- and male specific splice forms of doublesex (dsxF/dsxM) and Abdominal B (AbdB) (Supp Table 3) using the Stellaris RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA). dsxF mRNA was labeled with Quasar 570 ...
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bioRxiv - Cell Biology 2024Quote: ... We incubated samples with probes overnight at 30°C and proceeded to wash twice with the Stellaris RNA FISH Wash Buffer A followed by a single wash with the Stellaris RNA FISH Wash Buffer B (SMF-WA1-60 and SMF-WB1-20, Biosearch Technologies LGC, Middlesex, UK). Post-washing ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized cells were then resuspended in 100 μL hybridisation solution (Biosearch Technologies, SMF-HB1-10) containing 1-3x of standard probe concentrations for WHI5 and MDN1 probes ...
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bioRxiv - Bioengineering 2024Quote: ... one for flow cytometry and one for gDNA harvest with QuickExtract DNA Solution (BioSearch Technologies).
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from ∼100,000 cells using QuickExtract™ DNA Extraction Solution (Biosearch Technologies, QE0905T). The chromosome 5 enhancer locus was PCR amplified using Platinum™ II Hot-Start PCR Master Mix (2X ...
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bioRxiv - Bioengineering 2024Quote: ... one quarter of each well was pelleted for gDNA extraction using QuickExtract DNA Solution (BioSearch Technologies). The extracted gDNA was cleaned with 0.9x AmpureXP beads and genotyped by ddPCR ...
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bioRxiv - Bioengineering 2024Quote: ... Individual colonies were picked and gDNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). Knock-in was determined by ddPCR and INDELs were determined by ICE analysis as described above.
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). The following primer sequences were used to amplify the CCR5 locus around the sgRNA cut site as previously described:50
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bioRxiv - Bioengineering 2024Quote: ... cells were collected and genomic DNA was extracted using QuickExtract DNA extraction solution (Biosearch Technologies, cat.: QE09050). In-out PCR was performed with 3 forward primers and one reverse primer to selectively amplify knock-in events of the Ibalizumab ...
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bioRxiv - Genetics 2024Quote: ... We extracted the genomic DNA from FACS-isolated cells using QuickExtract DNA Extraction Solution (Biosearch Technologies, QE0905T). We amplified the allele with enhancer deletion from each genomic DNA using TaKaRa LA Taq DNA Polymerase (TaKaRa ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was extracted with QuickExtract DNA Extraction Solution (catalog no. QE09050; Lucigen, Biosearch technologies, Middleton, WI, USA) and used as the DNA template for PCR reactions performed using KAPA HiFi HotStart DNA Polymerase (catalog no ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA was extracted from the HEK293T and U2OS cells using the QuickExtract™ DNA Extraction Solution (Biosearch Technologies) system according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was extracted from 250,000 cells for each clonal cell line in 25 uL QuickExtractTM DNA Extraction Solution (BioSearch Technologies #QE09050) by thermocycling at 65°C for 6 minutes followed by 98°C for 2 minutes ...
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bioRxiv - Genomics 2023Quote: ... Genotyping of single colonies was performed after extraction of DNA with QuickExtract DNA Extraction Solution (Biosearch Technologies, catalog no. 76081-766) and PCR amplification with Q5® High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Immunology 2024Quote: Genomic DNA from 3E4-2E5 cells was isolated from arrayed samples using QuickExtract DNA Extraction Solution (Biosearch Technologies, catalog no. QE09050), using manufacturer’s protocol with reduced reagent volume to accommodate processing in a 96-well PCR plate ...
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bioRxiv - Biochemistry 2024Quote: ... Hybridisation was performed over-night at 37°C in hybridization solution (2× SSC, 10% formamide, 10% dextran sulphate) supplemented with SINV RNA-specific Stellaris (LGC Biosearch Technologies) and oligo(dT)25 (Life technologies Ltd ...
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bioRxiv - Biophysics 2024Quote: ... Genomic DNA was extracted from HEK293T cells by resuspending live or snap-frozen cell pellets in QuickExtract DNA Extraction Solution (Biosearch Technologies) followed by incubation at 65°C for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... The remaining 150 µL of T cells in culture were centrifuged at 300 × g for 5 minutes and the gDNA was harvested using QuickExtract DNA Solution (BioSearch Technologies) and analyzed by ddPCR as described above.
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bioRxiv - Developmental Biology 2024Quote: ... for 5 min at room temperature (RT) and incubated with 100 µL Stellaris Hybridization Solution (Biosearch Tech. cat. SMF-HB1-10) containing 0.91 µL hybridized smiFISH probes at 37°C for 30 min with shaking at 450 rpm in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed with Stellaris Wash Buffer A solution (2 mL Stellaris RNA FISH Wash Buffer A (Biosearch Technologies SMF-WA1-60), 7 mL Nuclease-free water ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested 2-3 days post-electroporation and genomic DNA (gDNA) was harvested using QuickExtract DNA extraction solution (Biosearch Technologies, Hoddesdon, UK, cat.: QE09050). To quantify knock-in alleles via ddPCR ...