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Citations for Anaspec :
1 - 50 of 104 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... T cells were stimulated by addition of SIINFEKL peptide (AnaSpec, AS-60193-1) for 4 hours and expanded for 3 days prior to an experiment ...
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bioRxiv - Cell Biology 2022Quote: ... and Hoechst 33258 nuclear stain (AnaSpec Inc; Exc/Em: 352⁄461 nm). NDs uptake and localization were evaluated via live-cell imaging by spinning-disk (SD ...
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bioRxiv - Immunology 2023Quote: ... Total anti-MOG IgG was quantified by using SensoLyte Anti-Mouse MOG(1–125) IgG Quantitative ELISA Kit (Anaspec).
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bioRxiv - Pharmacology and Toxicology 2021Quote: The ThT fluorescence assay was performed using the SensoLyte Thioflavin T β-Amyloid Aggregation Kit (ANASPEC, Fremont, CA, USA). Aβ and ThT solutions at final concentrations of 50 μM and 200 μM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Bioengineering 2019Quote: ... UltraPure grade Thioflavin T (ThT) (AnaSpec, Fremont, CA) was dissolved in deionized water at a concentration of 1 mM then filter-sterilized ...
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bioRxiv - Bioengineering 2019Quote: ... UltraPure grade Thioflavin T (ThT) (AnaSpec, Fremont, CA) was dissolved in deionized water at a concentration of 1 mM then filter-sterilized ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ThT assays were performed using the SensoLyte ® Thioflavin T β-Amyloid (1 – 42) Aggregation Kit (Anaspec, Cat no: AS-72214) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... fIX -18/+41 (the propeptide and Gla domain of factor IX, Anaspec), 1 uM ...
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bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Physiology 2020Quote: Peptides were synthesized using standard 9-fluorenylmethoxy carbonyl (Fmoc) protected and activated amino acids (Anaspec, CA, USA) on prederivatized cellulose membranes (Intavis AG ...
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bioRxiv - Cell Biology 2019Quote: ... differentiated SH-SY5Y cells were incubated with 5 μM TAT-TAMRA (AnaSpec) and 3 μM α-synPFF-AF488 either in the presence or in the absence of 250 μM polyP ...
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bioRxiv - Neuroscience 2023Quote: ... Aggregation was verified by Thioflavin T dye (AnaSpec, AS-88306) staining at 440Em/484Ex (38).
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...
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bioRxiv - Cell Biology 2022Quote: Fluorescein-5-Maleimide (AnaSpec) was attached to ubiquitin following the directions as previously described 40 ...
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bioRxiv - Microbiology 2019Quote: ... Next 5-TAMRA (4eq; Anaspec) was dissolved in 1 ml DMSO:NMP (1:1) ...
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bioRxiv - Genetics 2019Quote: ... separate cells were harvested and analyzed using the SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM 5-FAM-Lysine (Anaspec), prepared from a 20 mM stock in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 5-FAM-LC-LL-37 (AS-63694, Anaspec), Crotamine (CRO01-01000 ...
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bioRxiv - Molecular Biology 2021Quote: ... After the addition of 15 µl of 5× click reaction buffer (7.5 mM sodium ascorbate [Nacalai Tesque], 0.5 mM TBTA [AnaSpec], and 5 mM CuSO4) and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools) ...
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bioRxiv - Biophysics 2021Quote: ... 5-FAM fluorlabeled Crosstide was from AnaSpec (Fremont, CA). C1 peptide was from Promega (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4°C with rat anti-Mer (1:1000, eBioscience, cat# 14-5751-82) and rabbit anti-P2RY12 (1:2000, AnaSpec, cat# AS-55043A) in 2% NGS and 2% NDS in PBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP) was obtained from AnaSpec. Glutaraldehyde was purchased from Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 4μM of OVA (OVA 257-264, Anaspec Inc., Cat#AS-60193-5) or gp33 (gp33-41 ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin was further cross-linked with fluorescein-5-maleimide (Anaspec, Fremont, CA, US) and the poly-ubiquitination sample was generated following previous protocol 49 ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Cell Biology 2019Quote: ... following the manufacturer’s instructions (SensoLyte p300 Assay kit, SensoLyte PCAF Assay kit, Anaspec) using appropriate dilutions of NDGA and anacardic acid ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Biochemistry 2022Quote: N-terminal labeling of KSL-128114 with 5-(and-6)-carboxytetramethylrhodamine (TAMRA, Anaspec Inc.) was performed on resin ...
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bioRxiv - Biochemistry 2021Quote: ... a HABA biotin quantification kit (AnaSpec) was used to measure the biotin concentrations of each solution ...
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bioRxiv - Molecular Biology 2020Quote: ... Arabidopsis leaves of mature plants (5-6 weeks old) were infiltrated with either flg22 (1 μM; Anaspec) or H2O (mock ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was initiated by the injection of 5 µL of HCV NS3/4A protease substrate (AnaSpec) using a Perkin-Elmer EnVision plate reader ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ was examined using membrane potential sensitive fluorophore 3,3-dipropylthiadicarbocyanine iodide [DiSC3(5)] (Anaspec Inc, USA) (33) ...
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bioRxiv - Physiology 2024Quote: ... MW 30000–50000 Da) having 1 % of the monomers modified with 5-carboxytetramethylrhodamine (TAMRA, AnaSpec; PMAO-MNP). Further functionalization was performed by incubating 1 mg of Fe with 66 µmol of 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... 20 µM of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into 2x assay buffer [50 mM Tris at pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteolytic reaction was initiated by the injection of 5 μL of HCV NS3/4A protease substrate (AnaSpec), to a final concentration of 200 nM and kinetically monitored using a Perkin Elmer EnVision plate reader (excitation at 485 nm ...
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bioRxiv - Bioengineering 2023Quote: ... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Genetics 2020Quote: ... using the SensoLyte Malachite Green Assay kit (AnaSpec) in assay buffer (MgCl2 2 mmol/L ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of one of the glycopeptides (Muc5AC-3, Muc5AC-13, Muc5AC-3,13, Cst1.4, and Srs13.2 (Anaspec, Fremont, CA), 5 mM UDP-2-(acetylamino)-4-F-D-galactosamine disodium salt (UDP-GalNAc-F) ...
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bioRxiv - Cell Biology 2024Quote: ... ISCs (2,000 cells) and Paneth cells (2000 cells) were then seeded into 30 μl Matrigel containing 1 μM Jagged-1 (AnaSpec, San Jose, CA) and 10 μM Y-27632 ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Bioengineering 2024Quote: ... was determined via analysis of spent culture medium (n= 8-10/group) using a commercially available FRET-based generic MMP cleavage kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric, Anaspec, Fremont, CA). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Biochemistry 2021Quote: ... a 20 μM concentration of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into assay buffer [50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Microbiology 2023Quote: ... Malachite green reagent (SensoLyte MG Phosphate Assay Kit [AnaSpec]) was added to each reaction and incubated for 30 min before measuring absorbance at 660 nm ...