Labshake search
Citations for Anaspec :
101 - 138 of 138 citations for Pyruvate Kinase Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... C5a1 was stimulated with the FKP-(D-Cha)-Cha-r peptide agonist C028 (AnaSpec AS65121, in water). β2 was stimulated with isoprenaline (Sigma–Aldrich I5627 ...
-
bioRxiv - Biochemistry 2022Quote: ... Various concentrations of KDM5C were reacted with 25 nM H3K4me3 (1-21)-biotin peptide (AS-64357, AnaSpec) in 50 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2022Quote: BMDMs generated above were pulsed with GP100 (1µg/ml) or LCMV (1µg/ml, negative control) peptides (AnaSpec). Additionally ...
-
bioRxiv - Cancer Biology 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5 mmol/l.
-
bioRxiv - Bioengineering 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5Lmmol/l.
-
bioRxiv - Bioengineering 2023Quote: ... Me-HA was conjugated via Michael Addition with the integrin-binding RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a final concentration in the gel of 0.5 mmol/L ...
-
bioRxiv - Neuroscience 2022Quote: ... Pre-treatments included mixing Aβ42-HiLyteFluor-555 with 1 μg of blocking antibodies (α-Aβ42-Ab, Anaspec, Fremont, CA) for 15 min at room temperature prior to adding viral particles or pretreating the HSV-1-gB-GFP for 30 min at room temperature with 1% NP40 to disrupt the viral envelope ...
-
bioRxiv - Bioengineering 2022Quote: ... hMSCs were treated with 20 μM γ-secretase inhibitor DAPT or 20 μM Jag1 peptide (AnaSpec, 188-204) after osteogenic induction ...
-
bioRxiv - Biochemistry 2022Quote: ... Interacting proteins were eluted into buffer (25 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, 0.1 mg/mL FLAG peptide (Anaspec), and 10 µL/mL PMSF ...
-
bioRxiv - Neuroscience 2023Quote: ... injection of 10 mg/kg in saline of gp91-ds-tat peptide (AS-63818, AnaSpec Inc. Fremont, CA) every other day for one and two weeks ...
-
bioRxiv - Immunology 2022Quote: ... was incubated with the substrate of H3(1-21) lysine 9 tri-methylated biotinylated peptide (30 nM, Anaspec), in the presence of aKG (10 μM ...
-
bioRxiv - Immunology 2024Quote: ... BMDC were washed from LPS and added to the co-culture with the LCMV gp61-80 peptide (Anaspec) to a final concentration in co-culture of 2.5 ug/ml.
-
bioRxiv - Bioengineering 2024Quote: ... HAMe was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec, AS-62349) to obtain HAMe-RGD stock solutions used to make 15 mg/mL HA hydrogels that have a final RGD concentration of 0.15 mg/mL.
-
bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
-
bioRxiv - Immunology 2021Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated ...
-
bioRxiv - Immunology 2020Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated.
-
bioRxiv - Microbiology 2021Quote: ... pneumoniae cells were induced to competence by the addition of synthetic competence stimulatory peptide 1 (CSP-1; Anaspec, Inc.). Markerless deletions and replacements of target genes were constructed using the kanR-rpsL+ (Janus cassette ...
-
bioRxiv - Biochemistry 2020Quote: ... was used for detection of deacetylation activity with the acetylated peptide substrates derived from histone Bio-H4K12Ac (Anaspec #64849) and non-histone Bio-p53K382Ac (Anaspec #65046 ...
-
bioRxiv - Cell Biology 2023Quote: ... Each peptide is N-acetylated and C-amidated to reduce the effect of charged terminal residues. Fluorescein isothiocyanate (FITC)-labeled Tat peptide (FITC-LC-Tat, a.a. 47-57) was purchased from AnaSpec, Inc ...
-
bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
-
bioRxiv - Immunology 2021Quote: ... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
-
bioRxiv - Immunology 2021Quote: ... The following day recipient mice were immunized subcutaneously at tail base with 20 μg OVA323-339 peptide (Anaspec, Fremont, CA) emulsified in CFA (BD biosciences ...
-
bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
-
bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
-
bioRxiv - Biochemistry 2022Quote: ... 50 nM HAT module was incubated in buffer along with 0-700 mM H3 peptide (amino acids 1-21) (AnaSpec) at 30°C in 384-well plates (Greiner Bio-One ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were activated with bone marrow-derived DCs loaded for 1 h with indicated concentrations of N4 or Q4 OVA257-264 peptide versions (Anaspec) as previously described (69) ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
-
bioRxiv - Bioengineering 2023Quote: ... and then split in two aliquots: one third was pulsed with 50 µM OVA (257-264) peptide (Anaspec AS-60193-1) for 2 h at +37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
-
bioRxiv - Immunology 2021Quote: ... 5×106 lung cells were re-stimulated for 3 hours (37°C, 5% CO2) with 10 nM NP366–374 and PA224-233 peptides from Anaspec (Fremont, California) in the presence of Brefeldin/A from BD Biosciences (Franklin Lakes ...
-
bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...
-
bioRxiv - Immunology 2021Quote: ... a splenocyte suspension from one OT-I Rag2-/- spleen was stimulated with 10nM Ova257-264 peptide (SIINFEKL, AnaSpec, Cat no. 60193-5-ANA) for 48h and cultured for up to 10 days ...
-
bioRxiv - Microbiology 2021Quote: ... Both antibiotics were used as positive controls as they were commonly used for treating BK.1,4,62 The full-length peptides were commercially produced by Anaspec (Cambridge, UK; for LL-37) and PeproTech (London ...
-
bioRxiv - Immunology 2022Quote: ... Miltenyi Biotech) or BMDCs were stimulated for 3 h using Ova peptide 257–264 (SIINFEKL) (Cat no. AS-60193-5, AnaSpec, Fremont, CA, USA). For measuring antigen cross presentation ...
-
bioRxiv - Immunology 2019Quote: ... and stimulated for 3-5 hours at 37°C with equal concentrations of known immunodominant peptides gp10025-33 (KVPRNQDWL; JHU School of Medicine Synthesis & Sequencing Facility; Baltimore, MD) and Trp2180-188 (SVYDFFVWL; Anaspec Inc. Fremont, CA) or with control HA peptide (YPYDVPDYA ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were then probed for baboon APOL1 using anti-baboon APOL1 antibody (rabbit anti-sera raised against the following peptide: CSVEERARVVEMERVAESRTTEVIRGAKIVDK, AnaSpec Incorporated, 1:1000) and anti-Hp antibody (H8636 - 1:10,000) ...
-
bioRxiv - Immunology 2023Quote: ... Whole spleen cell stimulators from C57BL/6 mice were resuspended in RP10 media at 20×106 cells/mL pulsed with or without 100 nM OVA peptide sourced from AnaSpec (SIINFEKL, AS-60193-1) for 1 h at 37°C with gentle shaking every 15-20 minutes ...