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Citations for Anaspec :
101 - 150 of 158 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Genetics 2019Quote: ... H3(1-21) and H3(21-44) peptides were ordered from Anaspec, and H3(1-43 ...
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bioRxiv - Physiology 2021Quote: ... Di-4-ANEPPS (AnaSpec, Catalog# AS-84723, 1 µM in KH buffer), was perfused to image excitation of the heart ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies Rabbit anti-Vangl2 (1:100, Anaspec, AS-55659s, now discontinued) and Mouse anti β-II-Spectrin (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... the GLP-1 receptor agonist exendin-4 (10 nM, Anaspec (Fremont CA)) or the KATP-channel opener diazoxide (200 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Anaspec) were initially resuspended in 1% NH4OH (cat no: AS-61322, Anaspec) for 15 min to dissolve any pre-formed aggregates per manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Western Blot was performed using 1:500 dilution of Celsr1a antibody (AnaSpec. Inc).
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFP-tag (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... green (HiLyte Fluor 488) florescent β-amyloid (1 - 42) peptide (Anaspec, Fremont, CA) was used ...
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bioRxiv - Physiology 2022Quote: ... mixed with the peptide substrate library (Anaspec #AS-62017-1 and #AS-62335), kinase assay buffer I (SignalChem ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were stimulated by addition of SIINFEKL peptide (AnaSpec, AS-60193-1) for 4 hours and expanded for 3 days prior to an experiment ...
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bioRxiv - Neuroscience 2023Quote: ... UK) or rabbit anti-MMP2 (AS-55111, 1:250; AnaSpec, Fremont, CA, USA) primary antibodies diluted in FBS ...
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bioRxiv - Biophysics 2023Quote: ... biotinylated Dynorphin A 1-17 (Biotin – YGGFLRRIRPKLKWDNQ) were purchased from Anaspec (Fremont, CA). All solutions were prepared with 18.2 MΩ·cm water passed through a 0.2 μm polycarbonate Whatman filter and degassed to remove bubbles before use.
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were treated with 1 µm non-fluorescent Aβ1-42 (Anaspec #AS-20276) in the presence or absence of the hybrid protein in phenol red-free and serum-free DMEM ...
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bioRxiv - Immunology 2022Quote: ... OVA SIINFEKL peptide (257-264) (Cat no.-AS-60193-1) was purchased from Anaspec. Mouse CD8a+ isolation kit (Cat no.-130-104-075 ...
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bioRxiv - Neuroscience 2022Quote: ... Flore Rozenberg was mixed with 1 μg of Aβ42-HiLyteFluor-555 (Anaspec, Fremont, CA), with or without various pre-treatments (see below ...
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bioRxiv - Biochemistry 2020Quote: Stock solutions of HiLyte™ Fluor 488- labeled Human Aβ42 (Aβ, 0.1 mg; AnaSpec, USA) were prepared by dissolving the lyophilized peptide (1% NH4OH ...
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bioRxiv - Neuroscience 2021Quote: ... small peptide biomarker which is a substrate for MMP2/9 (A580, MMP Substrate 1, AnaSpec).
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bioRxiv - Molecular Biology 2019Quote: Dead cells in the fascicles were stained with Ethidium Homodimer-1 (2μM, AS 83208, Anaspec) previous to 10% formalin fixation (HT5011 ...
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bioRxiv - Immunology 2022Quote: ... 1 μM of OT-II peptide (chicken OVA amino acids 323-339 - Anaspec AS-27024) or TN peptide (β-hex peptide sequence YKGSRVWLN - GenScript)27 was added to the wells ...
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bioRxiv - Neuroscience 2022Quote: ... r-peptide) and biotin-tagged Aβ (1–42) (Cat# AS-23523-05, 0.5 mg, Anaspec) were used to make oligomeric forms as described in our previous study (Naito et al. ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stimulated in the presence of OVA257-264 (1 µg/ml) (Anaspec), recombinant murine IL-2 200 U/ml (Peprotech ...
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bioRxiv - Biochemistry 2021Quote: Human ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... mixed with the peptide substrate library in solution phase (Anaspec #AS-62017-1 and #AS-62335), and incubated for 90 mins (Assay conditions for each kinase described in Table S1)18–22 ...
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bioRxiv - Cell Biology 2023Quote: ... washed again 3X with PBS/1% NDS and lastly with PBS containing Hoechst33342 (AnaSpec, AS-83218) at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Arabidopsis leaves of mature plants (5-6 weeks old) were infiltrated with either flg22 (1 μM; Anaspec) or H2O (mock ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFAP (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... Various concentrations of KDM5C were reacted with 25 nM H3K4me3 (1-21)-biotin peptide (AS-64357, AnaSpec) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Blots were washed in 1XTBST and incubated with peroxidase-conjugated goat anti-rabbit (1:20,000, Anaspec, AS28177) for 4 hr at 4οC ...
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bioRxiv - Physiology 2024Quote: ... MW 30000–50000 Da) having 1 % of the monomers modified with 5-carboxytetramethylrhodamine (TAMRA, AnaSpec; PMAO-MNP). Further functionalization was performed by incubating 1 mg of Fe with 66 µmol of 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Biophysics 2021Quote: ... a series of 250 μL injections were used to clean the pepsin column: (1) Fos-choline-12 (Anaspec) in 0.1% TFA ...
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bioRxiv - Immunology 2022Quote: ... was incubated with the substrate of H3(1-21) lysine 9 tri-methylated biotinylated peptide (30 nM, Anaspec), in the presence of aKG (10 μM ...
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bioRxiv - Molecular Biology 2021Quote: Peptide-pull down was performed with synthetic peptide corresponding to H2A N-terminal (aa 1-23) obtained from AnaSpec. 1μg of peptide was incubated with 1μg of GST-tagged protein overnight on wheel at 4°C in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-treatments included mixing Aβ42-HiLyteFluor-555 with 1 μg of blocking antibodies (α-Aβ42-Ab, Anaspec, Fremont, CA) for 15 min at room temperature prior to adding viral particles or pretreating the HSV-1-gB-GFP for 30 min at room temperature with 1% NP40 to disrupt the viral envelope ...
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bioRxiv - Cancer Biology 2021Quote: ... post-transduction approximately 2×105 A2058 cells in 100µl warm PBS were transferred into tube and stained with 10 µg/ml of JC-1 dye (AnaSpec). An additional 100µl from control cells were treated with 200 µM FCCP ...
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bioRxiv - Immunology 2023Quote: ... Total anti-MOG IgG was quantified by using SensoLyte Anti-Mouse MOG(1–125) IgG Quantitative ELISA Kit (Anaspec).
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bioRxiv - Genetics 2024Quote: ... cells were expanded for 3 days in cRPMI media supplemented with 10 ng/mL OVA (Anaspec AS-60193-1), 5 ng/mL IL7 and 5 ng/mL IL15 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.05% Brij-35) and 1 µM of 520 MMP FRET substrate III (Cat. No. 60570-01; AnaSpec, San Jose, CA) was added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The procedure was performed as described by the manufacturer’s instructions (SensoLyte ® Thioflavin T Beta Amyloid (1-42) Aggregation Kit (Anaspec)) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4ºC with the primary antibody: rabbit polyclonal antibody against P2RY12 (1:250, #AS-55043A, AnaSpec Inc.). The secondary antibody was ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Biochemistry 2022Quote: ... 50 nM HAT module was incubated in buffer along with 0-700 mM H3 peptide (amino acids 1-21) (AnaSpec) at 30°C in 384-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were activated with bone marrow-derived DCs loaded for 1 h with indicated concentrations of N4 or Q4 OVA257-264 peptide versions (Anaspec) as previously described (69) ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrix solution was mixed 12:1 (v/v) with an internal calibrant mix containing 0.08 µg/ml Angiotensin I (Anaspec, Seraing, Belgium), 0.04 µg/ml Substance P (Anaspec ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ThT assays were performed using the SensoLyte ® Thioflavin T β-Amyloid (1 – 42) Aggregation Kit (Anaspec, Cat no: AS-72214) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and then split in two aliquots: one third was pulsed with 50 µM OVA (257-264) peptide (Anaspec AS-60193-1) for 2 h at +37°C ...
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...