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Citations for Anaspec :
1 - 50 of 76 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... containing 1 μM JAG-1 protein (AnaSpec AS-61298, NC0243900) in a flat bottom 48-well plate (Corning 3548) ...
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bioRxiv - Biochemistry 2019Quote: ... fIX -18/+41 (the propeptide and Gla domain of factor IX, Anaspec), 1 uM ...
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bioRxiv - Immunology 2023Quote: ... Total anti-MOG IgG was quantified by using SensoLyte Anti-Mouse MOG(1–125) IgG Quantitative ELISA Kit (Anaspec).
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent labeling of intermediate curli was performed using a HiLyte Fluor 555 protein labeling kit (Anaspec AS-72045). An aliquot of curli or curli-HiLyte555 was thawed from −80 °C ...
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bioRxiv - Bioengineering 2022Quote: ... ACE2-expressing HEK293T and HEK293T control cells were lysed and the activity of the ACE protein was assessed by the SensoLyte 390 ACE2 Activity Assay Kit (AnaSpec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... solutions containing OT (ANASPEC or KS-V Peptide) or AVP (ANASPEC or KS-V Peptide) ...
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bioRxiv - Immunology 2022Quote: ... with MMP-2 (AnaSpec) and Clostridium histolyticum collagenase (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Biochemistry 2022Quote: ... pH 6.5) containing 25 µM each Angiotensin-I (Anaspec, Fremont, CA), Angiotensin-II (Anaspec ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg/mL LL-37 (Anaspec), or 250 μg/mL kanamycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was replaced with MEF medium containing Aha (0.2 mM, Anaspec, #63669), instead of Met ...
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bioRxiv - Neuroscience 2022Quote: ... or 2 μg/mL fluorescent beta-amyloid (Anaspec). Image masks for fluorescence area and phase were generated using IncuCyte 2020C software.
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Neuroscience 2023Quote: ... the pre-equilibrated hypoxic media containing the peptide Adrenomedullin (ADM) (0.5 μM, Anaspec, AS-60447) +/- 10 uM ADM22-52 (the RAMP2/3 receptor blocker (Cayman Chemical Company ...
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bioRxiv - Neuroscience 2023Quote: The synthetic peptide Adrenomedullin (ADM) (2 nmol, Anaspec, AS-60447) was incubated with either ddH20 (for control condition ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mg of 2-Cl-Trt resin purchased from Anaspec was transferred to a 10 ml Torviq disposable reaction vessel and swelled for 1 hour in dichloromethane (DCM) ...
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bioRxiv - Cell Biology 2023Quote: ... washed again 3X with PBS/1% NDS and lastly with PBS containing Hoechst33342 (AnaSpec, AS-83218) at a 1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5 mmol/l.
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bioRxiv - Bioengineering 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5Lmmol/l.
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bioRxiv - Biochemistry 2019Quote: ... MGP 1-64 (the first 64 amino acids of matrix Gla protein, Anaspec), 1 uM ...
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bioRxiv - Cell Biology 2019Quote: ... following the manufacturer’s instructions (SensoLyte p300 Assay kit, SensoLyte PCAF Assay kit, Anaspec) using appropriate dilutions of NDGA and anacardic acid ...
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bioRxiv - Bioengineering 2024Quote: ... HAMe was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec, AS-62349) to obtain HAMe-RGD stock solutions used to make 15 mg/mL HA hydrogels that have a final RGD concentration of 0.15 mg/mL.
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bioRxiv - Neuroscience 2020Quote: ... and 2 μl of 20% Pluronic F-127 dissolved in DMSO (AnaSpec) and diluted in HBSS containing 10mg/ml BSA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (GSHWAVGHLM; 50 µg/mL: 2[Ser]-NMC, produced in AnaSpec; San Jose, CA) overnight at 4°C before use ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
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bioRxiv - Biochemistry 2021Quote: ... a HABA biotin quantification kit (AnaSpec) was used to measure the biotin concentrations of each solution ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrix solution was mixed 12:1 (v/v) with an internal calibrant mix containing 0.08 µg/ml Angiotensin I (Anaspec, Seraing, Belgium), 0.04 µg/ml Substance P (Anaspec ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of 25 μL of assay buffer containing substrate (FAM-SAVLQ/SG-QXL®520, AnaSpec, Fremont, CA). The substrate was derived from the cleavage sites on the viral polyproteins of SARS-CoV ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017). Confocal images were obtained on a Zeiss LSM 510 confocal microscope at the National Institute of Neurological Disorders and Stroke Light Imaging Facility ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... ISCs (2,000 cells) and Paneth cells (2000 cells) were then seeded into 30 μl Matrigel containing 1 μM Jagged-1 (AnaSpec, San Jose, CA) and 10 μM Y-27632 ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017)) ...
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bioRxiv - Bioengineering 2023Quote: ... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Genetics 2020Quote: ... using the SensoLyte Malachite Green Assay kit (AnaSpec) in assay buffer (MgCl2 2 mmol/L ...
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bioRxiv - Bioengineering 2024Quote: ... was determined via analysis of spent culture medium (n= 8-10/group) using a commercially available FRET-based generic MMP cleavage kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric, Anaspec, Fremont, CA). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Microbiology 2023Quote: ... Malachite green reagent (SensoLyte MG Phosphate Assay Kit [AnaSpec]) was added to each reaction and incubated for 30 min before measuring absorbance at 660 nm ...
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bioRxiv - Neuroscience 2019Quote: ... Single cells were re-plated onto matrigel treated coverslips at low density in the presence of 10nM PACAP protein (AnaSpec) or 0.01% DMSO (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... before addition of 1 mL of vesiculation buffer (GPMV buffer plus 25 mM PFA (paraformaldehyde, Electron Microscopy Services) and 2 mM DTT (1,4-dithiothreitol, Anaspec). Cells were incubated for 1 hour at 37°C and GPMVs were collected by removing supernatant from cells.
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bioRxiv - Microbiology 2020Quote: The SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Fremont, CA) was used according to the manufacturer’s instruction to measure the activity of the enzyme in each supernatant fraction obtained from the osmotic shock procedure (Tris/MgSO4 wash ...
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bioRxiv - Pathology 2022Quote: MMP-12 assay kit was purchased from AnaSpec (Fremont, CA). Frozen liver tissue (50-75 mg ...
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... The SensoLyte Red Glucocerebrosidase (GBA) Activity Assay Kit (AnaSpec, AS-72259) was used to determine enzyme activity in serum and whole tissue lysate samples ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...