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Citations for Anaspec :
151 - 199 of 199 citations for 8 11 Methano 10a 3 6a 1 propanyl 3 ylidene 8H indeno 2 1 b azocine 12 14 dione 5 acetyloxy 4 benzoyloxy dodecahydro 1 3 dimethyl 9 methylene 3R 4S 5R 6aR 6bR 8S 10aR 11R 11aS 15S 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were activated with bone marrow-derived DCs loaded for 1 h with indicated concentrations of N4 or Q4 OVA257-264 peptide versions (Anaspec) as previously described (69) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ThT assays were performed using the SensoLyte ® Thioflavin T β-Amyloid (1 – 42) Aggregation Kit (Anaspec, Cat no: AS-72214) with slight modifications to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and then split in two aliquots: one third was pulsed with 50 µM OVA (257-264) peptide (Anaspec AS-60193-1) for 2 h at +37°C ...
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bioRxiv - Cell Biology 2022Quote: Fluorescein-5-Maleimide (AnaSpec) was attached to ubiquitin following the directions as previously described 40 ...
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bioRxiv - Molecular Biology 2020Quote: Both cell lines were incubated with media supplemented with 200nM human Beta-Amyloid (1-42) HiLyte Fluor 555 (AnaSpec, Fremont, CA, USA), with or without thiethylperazine (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...
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bioRxiv - Microbiology 2019Quote: ... Next 5-TAMRA (4eq; Anaspec) was dissolved in 1 ml DMSO:NMP (1:1) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then probed for baboon APOL1 using anti-baboon APOL1 antibody (rabbit anti-sera raised against the following peptide: CSVEERARVVEMERVAESRTTEVIRGAKIVDK, AnaSpec Incorporated, 1:1000) and anti-Hp antibody (H8636 - 1:10,000) ...
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bioRxiv - Immunology 2022Quote: ... with MMP-2 (AnaSpec) and Clostridium histolyticum collagenase (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The activity of human MMP1 were measured by a SensoLyte® Plus 520 MMP-1 Assay Kit (AnaSpec, San Jose, CA, USA; AS-72012) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Whole spleen cell stimulators from C57BL/6 mice were resuspended in RP10 media at 20×106 cells/mL pulsed with or without 100 nM OVA peptide sourced from AnaSpec (SIINFEKL, AS-60193-1) for 1 h at 37°C with gentle shaking every 15-20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... animals were immunized subcutaneously in the flanks with 400µg of myelin oligodendrocyte glycoprotein 35-55 (MOG 33-55) (MEVGWYRSPFSRVVHLYRNGK; Anaspec Inc. Catalog No. AS-60130-1) in a 200ul emulsion of Complete Freund’s Adjuvant (4mg/ml Mycobacterium tuberculosis ...
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bioRxiv - Immunology 2019Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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bioRxiv - Bioengineering 2020Quote: ... 20 μg/ml biotin-4-fluorescein (AnaSpec, CA, USA) dissolved in PBS is added (step 3) ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg/mL LL-37 (Anaspec), or 250 μg/mL kanamycin (Sigma-Aldrich ...
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DIET INFLUENCES PERIPHERAL AMYLOID β METABOLISM: A ROLE FOR CIRCULATING INSULIN-LIKE GROWTH FACTOR IbioRxiv - Neuroscience 2020Quote: In vitro: Cells were treated during 15 hours with 500 nM soluble Aβ40-HiLyte Fluor™ 488 (AnaSpec) (68) ...
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bioRxiv - Cell Biology 2023Quote: ... 5-FAM-LC-LL-37 (AS-63694, Anaspec), Crotamine (CRO01-01000 ...
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bioRxiv - Bioengineering 2022Quote: ... and then with tetramethylrhodamine (TAMRA)-labeled Ang II (10-8 mol/L in PBS; AS-61181, AnaSpec, Fremont, CA) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... 5-FAM fluorlabeled Crosstide was from AnaSpec (Fremont, CA). C1 peptide was from Promega (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Neuroscience 2022Quote: ... or 2 μg/mL fluorescent beta-amyloid (Anaspec). Image masks for fluorescence area and phase were generated using IncuCyte 2020C software.
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bioRxiv - Biophysics 2020Quote: A super streptavidin biosensor (ForteBio, Menlo Park, USA) was coated with 15 μg/ml monomeric N-terminally biotinylated Aβ42 (AnaSpec, Fremont, USA) by overnight incubation a solution at 5 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Pre-acetylated Biotin-Histone 4 Peptide was purchased from AnaSpec (#64989-025). Bovine serum albumin fraction V (#A7888) ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
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bioRxiv - Biophysics 2021Quote: The preparation of α-Synuclein aggregates is followed from our previous work from Zhou and Kurouski.15 α-Syn (AnaSpec, CA, USA) was dissolved to the final concentration of 50 μM ...
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bioRxiv - Biochemistry 2022Quote: ... N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP) was obtained from AnaSpec. Glutaraldehyde was purchased from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: The synthetic peptide Adrenomedullin (ADM) (2 nmol, Anaspec, AS-60447) was incubated with either ddH20 (for control condition ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mg of 2-Cl-Trt resin purchased from Anaspec was transferred to a 10 ml Torviq disposable reaction vessel and swelled for 1 hour in dichloromethane (DCM) ...
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bioRxiv - Cell Biology 2019Quote: ... differentiated SH-SY5Y cells were incubated with 5 μM TAT-TAMRA (AnaSpec) and 3 μM α-synPFF-AF488 either in the presence or in the absence of 250 μM polyP ...
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bioRxiv - Immunology 2023Quote: ... 4μM of OVA (OVA 257-264, Anaspec Inc., Cat#AS-60193-5) or gp33 (gp33-41 ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin was further cross-linked with fluorescein-5-maleimide (Anaspec, Fremont, CA, US) and the poly-ubiquitination sample was generated following previous protocol 49 ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Biochemistry 2022Quote: N-terminal labeling of KSL-128114 with 5-(and-6)-carboxytetramethylrhodamine (TAMRA, Anaspec Inc.) was performed on resin ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μl of 20% Pluronic F-127 dissolved in DMSO (AnaSpec) and diluted in HBSS containing 10mg/ml BSA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (GSHWAVGHLM; 50 µg/mL: 2[Ser]-NMC, produced in AnaSpec; San Jose, CA) overnight at 4°C before use ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was initiated by the injection of 5 µL of HCV NS3/4A protease substrate (AnaSpec) using a Perkin-Elmer EnVision plate reader ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ was examined using membrane potential sensitive fluorophore 3,3-dipropylthiadicarbocyanine iodide [DiSC3(5)] (Anaspec Inc, USA) (33) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017). Confocal images were obtained on a Zeiss LSM 510 confocal microscope at the National Institute of Neurological Disorders and Stroke Light Imaging Facility ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Biochemistry 2019Quote: ... 20 µM of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into 2x assay buffer [50 mM Tris at pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteolytic reaction was initiated by the injection of 5 μL of HCV NS3/4A protease substrate (AnaSpec), to a final concentration of 200 nM and kinetically monitored using a Perkin Elmer EnVision plate reader (excitation at 485 nm ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017)) ...
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bioRxiv - Biochemistry 2021Quote: ... a 20 μM concentration of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into assay buffer [50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... before addition of 1 mL of vesiculation buffer (GPMV buffer plus 25 mM PFA (paraformaldehyde, Electron Microscopy Services) and 2 mM DTT (1,4-dithiothreitol, Anaspec). Cells were incubated for 1 hour at 37°C and GPMVs were collected by removing supernatant from cells.
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Immunology 2021Quote: ... a splenocyte suspension from one OT-I Rag2-/- spleen was stimulated with 10nM Ova257-264 peptide (SIINFEKL, AnaSpec, Cat no. 60193-5-ANA) for 48h and cultured for up to 10 days ...