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Citations for Anaspec :
101 - 150 of 179 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... splenocytes were isolated and stimulated with 10 nM OVA257-264 peptide (AnaSpec, CA, USA) in complete media containing Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... OVA SIINFEKL peptide (257-264) (Cat no.-AS-60193-1) was purchased from Anaspec. Mouse CD8a+ isolation kit (Cat no.-130-104-075 ...
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bioRxiv - Cell Biology 2021Quote: ... splenocytes were isolated and stimulated with 10nM OVA257-264 peptide (AnaSpec, Fremont, CA, USA) in 10% RPMI (RPMI 1640 plus 10% fetal bovine serum (Fisher) ...
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bioRxiv - Immunology 2020Quote: ... or complete RPMI-1640 supplemented with LCMV GP61-80 peptide GLKGPDIYKGVYQFKSVEFD (5μg/mL, Anaspec) or HEL (0.5μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... control mice were inoculated with either PBS or the parental peptide LL-37 (Anaspec).
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bioRxiv - Neuroscience 2021Quote: ... small peptide biomarker which is a substrate for MMP2/9 (A580, MMP Substrate 1, AnaSpec).
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bioRxiv - Immunology 2022Quote: ... 1 μM of OT-II peptide (chicken OVA amino acids 323-339 - Anaspec AS-27024) or TN peptide (β-hex peptide sequence YKGSRVWLN - GenScript)27 was added to the wells ...
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bioRxiv - Neuroscience 2022Quote: ... r-peptide) and biotin-tagged Aβ (1–42) (Cat# AS-23523-05, 0.5 mg, Anaspec) were used to make oligomeric forms as described in our previous study (Naito et al. ...
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bioRxiv - Neuroscience 2023Quote: ... the pre-equilibrated hypoxic media containing the peptide Adrenomedullin (ADM) (0.5 μM, Anaspec, AS-60447) +/- 10 uM ADM22-52 (the RAMP2/3 receptor blocker (Cayman Chemical Company ...
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bioRxiv - Bioengineering 2023Quote: ... plated at 800,000 splenocytes/well and restimulated with 10 μg rabies virus glycoprotein peptide (AnaSpec) for 24h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Cells were stimulated with 2 μg/mL of SIINFEKL peptide (Anaspec, cat. AS-60193-5) and 1 mg/mL of Brefeldin A (Invitrogen ...
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bioRxiv - Systems Biology 2022Quote: ... mixed with the peptide substrate library in solution phase (Anaspec #AS-62017-1 and #AS-62335), and incubated for 90 mins (Assay conditions for each kinase described in Table S1)18–22 ...
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bioRxiv - Physiology 2020Quote: Peptides were synthesized using standard 9-fluorenylmethoxy carbonyl (Fmoc) protected and activated amino acids (Anaspec, CA, USA) on prederivatized cellulose membranes (Intavis AG ...
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bioRxiv - Biochemistry 2022Quote: ... 60 µM of ZHX2 (ASKRKSTTPCMVRTS) or 0.75 µM of RPS25 (PPKDDKKKKDAGKS) N-terminal peptides (Anaspec, Fremont, CA) as substrates ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide P29 (YGG ASV CIY CRS RVE HPD VDG LCK LRG KF-NH2) was synthesized by AnaSpec Inc./Kaneka Eurogentec (EGT Corporate ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... C5a1 was stimulated with the FKP-(D-Cha)-Cha-r peptide agonist C028 (AnaSpec AS65121, in water). β2 was stimulated with isoprenaline (Sigma–Aldrich I5627 ...
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bioRxiv - Biochemistry 2022Quote: ... Various concentrations of KDM5C were reacted with 25 nM H3K4me3 (1-21)-biotin peptide (AS-64357, AnaSpec) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: BMDMs generated above were pulsed with GP100 (1µg/ml) or LCMV (1µg/ml, negative control) peptides (AnaSpec). Additionally ...
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bioRxiv - Cancer Biology 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5 mmol/l.
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bioRxiv - Bioengineering 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5Lmmol/l.
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bioRxiv - Cancer Biology 2024Quote: ... GST-CBP (1081-1197) was incubated with the non-acetylated biotin-H4 (1-21) peptide (AnaSpec. 62555) and in the presence of 0.25% of final DMSO concentration ...
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bioRxiv - Bioengineering 2022Quote: ... hMSCs were treated with 20 μM γ-secretase inhibitor DAPT or 20 μM Jag1 peptide (AnaSpec, 188-204) after osteogenic induction ...
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bioRxiv - Biochemistry 2022Quote: ... Interacting proteins were eluted into buffer (25 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, 0.1 mg/mL FLAG peptide (Anaspec), and 10 µL/mL PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... injection of 10 mg/kg in saline of gp91-ds-tat peptide (AS-63818, AnaSpec Inc. Fremont, CA) every other day for one and two weeks ...
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bioRxiv - Immunology 2022Quote: ... was incubated with the substrate of H3(1-21) lysine 9 tri-methylated biotinylated peptide (30 nM, Anaspec), in the presence of aKG (10 μM ...
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bioRxiv - Immunology 2024Quote: ... BMDC were washed from LPS and added to the co-culture with the LCMV gp61-80 peptide (Anaspec) to a final concentration in co-culture of 2.5 ug/ml.
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bioRxiv - Bioengineering 2024Quote: ... HAMe was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec, AS-62349) to obtain HAMe-RGD stock solutions used to make 15 mg/mL HA hydrogels that have a final RGD concentration of 0.15 mg/mL.
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bioRxiv - Immunology 2024Quote: MDMi treated with various conditions were incubated with 10 uM HyLite Fluor 647-labeled Aβ1-42 peptide (AnaSpec) for 1 hour at 37°C.
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bioRxiv - Immunology 2024Quote: ... 5% CO2 incubator) in TCM in the presence of 1μg/mL GP3333-41(KAVYNFATM) peptide (#AS-61669, Anaspec) for 4 h and with (1:1500 dilution ...
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bioRxiv - Immunology 2021Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated ...
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bioRxiv - Immunology 2020Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated.
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bioRxiv - Microbiology 2021Quote: ... pneumoniae cells were induced to competence by the addition of synthetic competence stimulatory peptide 1 (CSP-1; Anaspec, Inc.). Markerless deletions and replacements of target genes were constructed using the kanR-rpsL+ (Janus cassette ...
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bioRxiv - Biochemistry 2020Quote: ... was used for detection of deacetylation activity with the acetylated peptide substrates derived from histone Bio-H4K12Ac (Anaspec #64849) and non-histone Bio-p53K382Ac (Anaspec #65046 ...
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bioRxiv - Cell Biology 2023Quote: ... Each peptide is N-acetylated and C-amidated to reduce the effect of charged terminal residues. Fluorescein isothiocyanate (FITC)-labeled Tat peptide (FITC-LC-Tat, a.a. 47-57) was purchased from AnaSpec, Inc ...
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bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... The following day recipient mice were immunized subcutaneously at tail base with 20 μg OVA323-339 peptide (Anaspec, Fremont, CA) emulsified in CFA (BD biosciences ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were activated with bone marrow-derived DCs loaded for 1 h with indicated concentrations of N4 or Q4 OVA257-264 peptide versions (Anaspec) as previously described (69) ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Biochemistry 2022Quote: ... 50 nM HAT module was incubated in buffer along with 0-700 mM H3 peptide (amino acids 1-21) (AnaSpec) at 30°C in 384-well plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... This was achieved by incubating the protein with 1 mg/ml S. aureus sortase A (a gift from H. Ploegh, MIT)60 and 100 μM peptide (LPETGG) conjugated with FAM (Anaspec) for 2 h at RT protected from light ...
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A gated hydrophobic funnel within BAX binds long-chain alkenals to potentiate pro-apoptotic functionbioRxiv - Cell Biology 2024Quote: Additional recombinant proteins and peptides were purchased from commercial sources: 5-TAMRA labeled BAK-BH3 (Cat. No. AS-64590, AnaSpec); recombinant Bcl–xLΔC ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Bioengineering 2023Quote: ... and then split in two aliquots: one third was pulsed with 50 µM OVA (257-264) peptide (Anaspec AS-60193-1) for 2 h at +37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The kinase reaction was initiated by the addition of ATP (10 µM final) and fluorescein labeled Scrtide peptide (AnaSpec, Fremont, CA)(1 µM final) ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
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bioRxiv - Immunology 2021Quote: ... 5×106 lung cells were re-stimulated for 3 hours (37°C, 5% CO2) with 10 nM NP366–374 and PA224-233 peptides from Anaspec (Fremont, California) in the presence of Brefeldin/A from BD Biosciences (Franklin Lakes ...
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bioRxiv - Immunology 2022Quote: ... in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1], or TSYKFESV [Anaspec, #AS-64688]) at a final concentration of 2 µM for 5 hours prior to Fc block as before ...