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Citations for SignaGen Laboratories :
51 - 100 of 106 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the corresponding plasmids were transfected using Poly Jet™ transfection reagent (SignaGen Laboratories, SL100688) after the 293T cells had been grown to 80% confluence for 18-24 h before treatments ...
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bioRxiv - Cancer Biology 2020Quote: ... Mutant plasmids were directly transfected into cell lines using GeneJet transfection reagent (SignaGen Labs) or packaged in 293T for lentiviral infection.
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bioRxiv - Microbiology 2020Quote: 293T cells were transiently transfected with GenJetTM In Vitro DNA Transfection Reagent (SignaGen Laboratories). Luciferase reporter assays were performed 24 h after DNA transfection ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected with RILPL1-miniSOG-HA using LipoD293™ Transfection Reagent (SignaGen Laboratories, SL100668) as described above ...
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bioRxiv - Systems Biology 2023Quote: ... 15 μg of Strep-tagged plasmids was combined with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid:transfection reagent ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA mixtures were complexed with PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio (plasmid:transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G and psPAX2 in 2:1:2 ratio with PolyJet transfection reagent (SignaGen Laboratories). Media were harvested two days later and added to recipient cells with 1 μg/ml polybrene (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection of HEK293T or HCT116 cells was carried out using LipoD293 (SignaGen Laboratories, Rockville, MD) or FuGENE HD (Promega ...
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bioRxiv - Genetics 2021Quote: ... then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SignaGen Laboratories, SL100668) at 24–48 hours before the imaging studies ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng pCMV-IFNL2-genomic or pCMV-IFNL2-cDNA using PolyJet transfection reagent (SignaGen labs) according to the manufacturer’s DNA transfection protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... packaging plasmid (Δ8.9) and envelope (VSVG) vectors using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... packaging plasmid (Δ 8.9) and envelope (VSVG) vectors using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... packaging plasmid (Δ8.9) and envelope (VSVG) vectors using Lipo 293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with plasmid DNA using PolyJet DNA In Vitro Transfection Reagent (Signagen Laboratories, Gaithersburg, USA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: HEK-293T and A549 cells were transfected with LipoJet™ In Vitro Transfection Kit (SignaGen Laboratories, SL100468) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using GenJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories, catalog # SL100489-OS) for U2OS Cells as instructed ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were transfected with GFP-TDP-43 by PolyJet™ DNA in vitro transfection reagent (Signagen Laboratories). Cells were seeded into a 6-well plate at a density of 5 × 105 cells/well and incubated overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using Genefect (Alkali Scientific, Fort Lauderdale, FL) or LipoJet transfection reagents (Signagen Laboratories, Rockville, MD), according to each manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... Total plasmid was normalized to 15 μg with empty vector and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Total plasmid was normalized to 15 μg with vector DNA and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg ...
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bioRxiv - Molecular Biology 2023Quote: The oligonucleotides were delivered using the GenMute™ siRNA transfection reagent for primary macrophages (SL100568-PMG, SignaGen Laboratories), according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Total plasmid was normalized to 15 μg with empty vector and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... COMMA1D cells were then transfected with INSR knock-out plasmid using 15 µL PolyJet transfection reagent (SignaGen Laboratories #SL100688). Following transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the appropriate amount of DNA plasmid according to the manufacturer’s protocol using GenJet U2OS DNA in vitro transfection reagent (SignaGen Laboratories) for 18 to 24 hrs.
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bioRxiv - Neuroscience 2020Quote: pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in a 1:1 mixture of DNA/Polyjet DNA transfection reagent for 5-18 hours according to the manufacturer’s protocol (SignaGen Laboratories). Expression of plasmid DNA was selected using G418 ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T and HEK293T-NnSULT1C1 cells were transfected with pAcBacw.tR4-sTyrRS/GFP* with Polyjet In Vitro DNA Transfection Reagent (SignaGen Laboratories) in the presence or absence of the indicated concentration of sTyr ...
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bioRxiv - Molecular Biology 2023Quote: ... U2OS and HEK cells were transfected with the appropriate amount of DNA plasmid according to the manufacturer’s protocol using GenJet U2OS or Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) for 18 to 24 hrs.
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bioRxiv - Neuroscience 2020Quote: The lentiviral vector for eGFP under control of the human EF1α-promoter was purchased from SignaGen Laboratories (Gaithersburg MD, USA). All other lentiviral vectors were made in the department of Prof ...
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bioRxiv - Immunology 2020Quote: ... RNase L cleaved small RNAs and control small RNAs were prepared as previously (46, 72) and transfected (2µg/ml) using Polyjet reagent (SignaGen Laboratories) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with 30 μg full-length proviral plasmid DNA containing the WT sequence or indicated CA mutations using PolyJet DNA transfection reagent as recommended by the manufacturer (SignaGen Laboratories). Two days after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... SiHa cells at 24 h of cell passage were transfected with 40 nM of siRNA by LipoJet In Vitro Transfection Kit (Ver. II) (SignaGen Laboratories). Total protein extracts and total RNA were prepared 48 h after the transfection.
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bioRxiv - Microbiology 2019Quote: ... DNA containing the WT sequence or indicated pol mutations within IN coding sequence using PolyJet DNA transfection reagent as recommended by the manufacturer (SignaGen Laboratories). Two days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The SH-SY5Y neuroblastoma cells were transfected with 10 ug of plasmid DNAs when 60% confluence in 100-mm dishes using transfection reagents GenJet II (SignaGen Laboratories, Rockville ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg of a gel-purified mutagenic primer targeting mouse rs11031006 (5’-CTGGAATTTAATATTGCTCTGCCCTGTGATATTTATTTCAAGGTTAGTAGAAATGTAGCTACCTCCTGTAATGACAAATGA-3’) using PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories). At 18 hours post transfection ...
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bioRxiv - Biophysics 2021Quote: ... Up to 15 μg of individual Strep-tagged expression constructs (normalized to 15 μg with empty vector as needed) was complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T were transfected with PB-NnSULT1C1 (100 ng) and Piggybac transposases plasmids (20 ng) with Polyjet In Vitro DNA Transfection Reagent (SignaGen Laboratories). 1 μg/mL puromycin was added to culture medium from Day 2 to Day 7 for selecting cells with genomic integration of NnSULT1C1 ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral vectors were assembled using a 3rd generation lentiviral system and CalFectinTM Mammalian DNA Transfection Reagent (SignaGen Laboratories, Rockville, MD) in 293T cells according to the manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: Cell extracts for oligo pulldown assays were isolated from HEK293T cells transfected with an ORF57-expressing vector (pVM7) using LipoD293 transfection reagent (SignaGen Laboratories). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X 150 mm dish of HEK293 cells were transfected with either FLAG-HA-YTHDC1 or FLAG-ZFC3H1 (1-1233) using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories). 18 to 24 hours post transfection the cell pellets were trypsinized ...
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bioRxiv - Neuroscience 2021Quote: ... and transfected at 80 % confluence with 1 μg of LRRK2 constructs (and 100 ng of HA-tagged vps35 constructs where indicated) and 3 μl of LipoD293™ Transfection Reagent (SignaGen Laboratories) per well of a 12-well plate overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus was produced according to the “General Protocol for Generation of lentivirus in 293T cells” using “PolyJet™ In Vitro DNA Transfection Reagent” (Signagen Laboratories). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cells were grown to 60% confluency then transfected with 3 μg of each relevant DNA construct using the PolyJetTM transfection reagent (SignaGen Laboratories, USA). 6 hours later ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells at 0.25 × 106 were plated into 6-well plates 24 h before the first siRNA transfection with 40 nM of the ON-TARGETplus human siRNA SMARTpool (Horizon Discovery Biosciences) using LipoJet (SignaGen Laboratories, Frederick, MD) as recommended by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated at ∼30% confluency into 8-well chambered slides ∼24 h prior to measurement and transfected with vectors as indicated in the text ∼16 h prior to measurement using GenJet transfection reagent (SignaGen Laboratories, Fredrick, MD) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DsiRNAs were transfected into Vero cells growing on 35 mm glass bottom dishes or on standard 35 mm dishes using PepMute transfection reagent (SignaGen Laboratories, Rockville, MD) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... B16-F1 cells were transfected with 1 μg DNA of pCMV3 vector encoding IL-6 or the empty control pCMV3 vector (EV) using PolyJet (SignaGen Laboratories, MD, USA) following the manufacturer’s instructions ...