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Citations for abcam :
401 - 450 of 2130 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were blocked for 30 min with 1% BSA previous 1h incubation at RT with the primary antibody rabbit anti-pericentrin (Abcam, ab4448), rabbit anti-pericentrin (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... A commercial kit (Immunoprecipitation Kit, Abcam), which provided buffers for protein extraction and solubilization as well as Protein A/G sepharose beads and washing buffers ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 3 times with PBST and goat anti-pig IgG HRP antibody (Abcam) diluted 1:10,000 in PBST containing 1% FBS was added to the well (60 μl/well) ...
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bioRxiv - Cell Biology 2022Quote: ... Potassium green (KG, 10μM, ION Potassium-Green-2 AM, K+ indicator, abcam ab142806; 45min incubation time), a lipophilic styryl dye (5μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed two times with ddH2O and counterstained using hematoxylin solution (Abcam cat. no. ab220365) or counterstain supplied in kit for 1-2 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times in PBST and incubated in 1μM DAPI solution (#ab228549, Abcam) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed in 1xPBST three times (5mins) and mounted with Fluoroshield with DAPI (Abcam, ab104139). Images were taken using the Leica DM7200 fluorescence imaging platform with LAS X version 3.7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Horseradish peroxidase-labeled conformation-sensitive secondary anti-mouse IgG for IP or anti-rabbit VeriBlot for IP Detection antibody (both at 1:20000; Abcam, Cambridge, MA) was detected by SuperSignal West Femto Maximum Sensitivity substrate (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... three pre-diluted primary antibodies were sequentially applied in the following order using the indicated chromogenic detection: anti-CASQ1 (1:10000, Abcam # ab185220, Abcam, Cambridge, UK) with Omap anti-Ms HRP (Ventana Discovery #760-4310 ...
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bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and a polyclonal rabbit serum against SARS-CoV-2 spike was added and incubated for 1 hour following a wash step and detection of binding with goat anti rabbit-HRP (Abcam catalog # ab97080). TMB substrate was added to the plates for detection of HRP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated under darkness at RT with a goat anti-mouse secondary antibody (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Cell Biology 2020Quote: ... slides were blocked with PBS + 1% BSA + 2% goat serum + 0.2% Triton X-100 for 1h at RT and incubated overnight at 4◦C with anti-DCLK1 antibody (Abcam #ab31704; 1:250) in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed in 1X PBS and then incubated in a blocking solution containing 3% normal goat serum and 0.3% triton X-100 in 1X PBS for 60 mins at RT prior to incubation with Laminin (Abcam #ab11575; 1:200) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 2.5 hours at RT using two different anti-BrdU antibodies recognizing CldU (Abcam, ab6326, 1:500) and IdU (Becton Dickinson ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently incubated for 1h at RT with the secondary antibody diluted in the blocking buffer solution: Donkey anti Rabbit 647 (1:1000, AB150075, ABCAM, Cambridge, UK); Donkey anti Mouse 594 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Immunology 2023Quote: ... The slides were then blocked with 10% normal goat serum phosphate-buffered saline (PBS) for 30 min at room temperature (RT) and then were incubated with either Hamster anti-PDPN (Abcam Cat#: ab11936) or Rabbit anti-proSP-C (Millipore Sigma Cat# ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... the Catalase kit buffer (Abcam, kit ab83464) for catalase activity ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times as previously and goat anti-human IgG-HRP secondary antibodies (Abcam, ab97215) were added at 1:25,000 and incubated 30 min at room temperature ...
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bioRxiv - Pathology 2021Quote: ... and rabbit anti-mouse α-smooth muscle actin (SMA) antibody (3000-time dilution: Abcam plc, Tokyo, Japan) were used ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times with TBST before incubation with HRP-conjugated secondary antibodies (1/20,000, Abcam) for 45min at RT in 2% milk TBST.
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bioRxiv - Immunology 2022Quote: ... Cells were washed 3 times in PBS and incubated with a mouse secondary antibody (1:500, Abcam) and DAPI (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... then washed three times in PBT 0,1% and incubated with goat anti-rabbit FITC antibody (Abcam #6717) diluted 1:1000 in PBT 0,1% + BSA 2% ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed six times with TBS-T then probed with horseradish peroxidase-conjugated secondary antibodies (Abcam) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Serum samples from every time point were analysed for malondialdehyde (MDA) using ELISA methodologies (Abcam, Cambridge UK). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... the coverslips were washed three times with PBS and incubated with mouse and rabbit secondary antibodies (Abcam) (diluted 1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... IRDye 800CW donkey anti-Rabbit IgG (LiCOR Biosystems cat. no. 926-32213) and VeriBlot for immunoprecipitation detection reagent (HRP) (Abcam; catalog no. ab131366). Secondary antibodies for Western blot were diluted at either 1:5000 (HRP-conjugated ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA detection was performed by applying 2 ng of the sample used for PicoGreen detection to a nylon membrane and detecting with the anti-dsDNA antibody (Abcam #ab27156, 1:9000).
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were washed with TBS-T for 15 minutes three times and incubated with secondary antibody (Abcam, ab97051) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed five times with PBS-T and 100 μL of TMB ELISA Substrate Solution (Abcam, catalogue number ab171525 ...