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Citations for abcam :
351 - 400 of 2130 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1hr at RT with a horseradish peroxidase-conjugated anti-rabbit goat serum (1:10000; Abcam ab6721). After washing ...
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bioRxiv - Cell Biology 2020Quote: ... gels were incubated 24 hours at RT on a rocking platform with rabbit anti-TOM20 antibody (Abcam, catalog no. ab78547). Gels were then washed in PBS-T (0.1% Tween 20 in 1× PBS ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 min at RT and then overnight at 4 °C in a humidified chamber with the following antibodies: LC3 (1:50, Abcam), p62 (1:20 ...
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated with 3% BSA and 0.1% Triton X-100 for 30 min at RT and stained with anti-gamma H2AX (Abcam 2893), anti CENP B (Santa Cruz ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently incubated for 1h at RT with Alexa-488-coupled anti-LAMP1 antibody (1:500 in PBS containing 1% BSA, Abcam). Cells were washed twice in PBS and stored at 4°C in the dark.
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bioRxiv - Cancer Biology 2020Quote: ... The dishes were then incubated with blocking buffer ar RT for 1 hr and immediately the primary antibody (Coilin, ab87913; PML, ab96051; RNAPII, ab193468; Abcam) diluted in antibody diluent was applied and incubated at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in blocking solution containing primary antibodies for 1 hr at RT (EnCOR Chicken-anti-GFAP at 1:2000, Abcam Rabbit-anti-pRab10 at 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450 ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Genetics 2020Quote: ... Incubation of the secondary antibody was performed for 1h at RT with either rabbit anti-mouse (abcam # ab46540, for H3K27me3) or guinea pig anti-rabbit antibody(Antibodies online #ABIN101961 ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... Brain slices were then incubated for ∼20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry ...
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bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 3h at RT using two different anti-BrdU antibodies recognizing CldU (#ab6326, Abcam) and IdU (#347580 ...
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bioRxiv - Cancer Biology 2023Quote: ... at rt for 1 h and blotted with the following primary antibodies at 4°C overnight: β-Actin (Abcam, ab8224), MDR1/ABCB1 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody staining was done sequentially in PBS/1% BSA at RT: rabbit anti-GFP (1:500, 1 h, Abcam, 6556), goat anti-rabbit IgG conjugated to 18 nm gold (1:20 ...
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bioRxiv - Genomics 2023Quote: ... Cells were then incubated with the primary antibody (diluted in 0.5% BSA/PBS) at RT for 1h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1,000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with the following primary antibody diluted in PBS-Tween (0.1%) for 1 h at RT: anti-Olig2 (1:100, Abcam, #ab109186), anti-Cleaved caspase 3 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed with PTX for 3 × 10 min at RT and incubated with secondary antibodies in PTX (1:200 goat anti-chicken IgY H&L DyLight 488 (Abcam)) for 2 h at RT ...
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bioRxiv - Biophysics 2022Quote: ... Mycoplasma contamination was ruled out by PCR (Abcam 289834).
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bioRxiv - Cell Biology 2020Quote: ... the following reagents were used for indicated periods of time – (1) SCD inhibitor (Abcam, ab142089) – 4 μM in DMSO (2 ...
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bioRxiv - Physiology 2020Quote: ... Tissue was rinsed 3 times with PBST before secondary was applied (Goat antirabbit 647, Abcam) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed 3 times with PBS and incubated in 3,3ʹ-Diaminobenzidine (DAB) chromogen (Abcam).Slides were then dehydrated in increasing grades of alcohol (70% ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were stained with a 1:10 dilution of individual animal serum followed by detection with a 1:1000 dilution of a goat anti-mouse Fc FITC secondary (Abcam ab98716) and fixation with 4% formalin ...
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were pelleted and washed with PBS twice and then co-incubated in detection antibody (donkey anti rabbit IgG-AF488: Abcam[Ab150093])) diluted 1:5000 in PBS and incubated for 15 minutes at 4 degrees ...
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bioRxiv - Biochemistry 2023Quote: ... Nucleosomes bound were detected using western blot followed by detection with histone antibodies (anti-H3 Abcam ab1791; anti-H2A Abcam ab18255, anti-H2B Abcam ab1790) and HRP conjugated secondary antibodies (anti-mouse IgG-HRP Vector Labs PI2000 ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Microbiology 2020Quote: ... Cloned IRE1-/- MEF cells were validated by RT-qPCR using specific primers and by immunoblot using IRE1-specific antibody (Abcam-ab37073). IRE1-/- cells were complemented with pcDNA3-derived vectors containing WT or the indicated mutant versions of IRE1 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rehydrated in PBS 3 times for 10 minutes and blocked with 7% normal goat serum in PBS for 1 hour and then incubated overnight at room temperature (RT) with anti-cFos (1/200, rabbit, Abcam ab209794) or anti-Cleaved-Caspase 3 (1/400 ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2019Quote: ... 1% goat serum with Triton was used as a blocking solution for at least 1 hour at RT and a Goat Anti-Rat IgG H&L (HRP) (Abcam ab97057) was used as a secondary and incubated for 1.5 hours at RT before continuing with the described DAB staining ...
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bioRxiv - Molecular Biology 2019Quote: ... coverslips were blocked with 4% BSA/PBS for 45 min at RT and primary antibody anti-rabbit lamin B1 (abcam ab16048), mab414 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of N2a cells samples were incubated for 1 hour at room temperature (RT) with primary antibodies for ATP5A (1:1000, Abcam Ab119688) and GAPDH (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 0.1% Triton X-100/TBS 3 times and incubated in secondary antibody and phalloidin for 1 h at room temperature (RT) (in Abdil, 1:2000 Alexa Fluor 488 Donkey Anti-Goat (Abcam ab150129) and 1:250 Phalloidin Atto 647N (Sigma-Aldrich 65906) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hr at RT and incubated in PBST overnight at 4°C with following primary antibodies: chicken anti-GFP (1:1000, Abcam ab13970), chicken anti-MAP2 (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by washing in PBS (3 × 15 min) and an overnight incubation (>16 hr at RT) with the primary antibody (1:1000, rat anti-Ctip2, Abcam #ab18465) diluted in antibody solution (1% goat serum and 0.2% Triton X-100 in PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Same experiment was also performed blocking monocyte-derived macrophages at RT for 15 min with 10 μg/ml of mAb α-Siglec-1 7–239 (Abcam), IgG1 isotype control (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
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bioRxiv - Cell Biology 2019Quote: ... at RT for 1 h and incubated with the following primary antibodies overnight at 4°C: SUN2 (1:100; rabbit; Abcam ab124916), total β1-integrin (1:50 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then treated with the blocking solution (10% Goat serum) for 1 h at RT and incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Immunology 2023Quote: ... was incubated with the transferred proteins for 2 h and the binding was detected after incubation for 1 h at RT with HRP-conjugated anti-HA antibody diluted 1:5000 (Abcam, Ab1190). The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... preferably filtered) was added for 15 min at RT before primary rb-anti-TMC3 (ATLAS HPA040265) and ms-anti-Beta3TUB (ABCAM, ab7751) antibody was added at 1:200 and VG cells were incubated at 4° C O/N on a lightly tilting tissue culture platform ...
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bioRxiv - Biochemistry 2023Quote: ... 1% BSA in 0.5% PBST) for 1h at RT prior to the addition of primary antibodies (isoDGR [1:200] and CD68 [1:200, Abcam, ab283654]) overnight at 4 °C ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... blocked in 1% bovine serum albumin in PBS for 1 h at RT and incubated with anti-pERM antibody (Abcam, ab76247), anti-alpha-tubulin-FITC (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...