Labshake search
Citations for abcam :
401 - 450 of 2168 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
-
Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... Brain slices were then incubated for ∼20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry ...
-
bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 3h at RT using two different anti-BrdU antibodies recognizing CldU (#ab6326, Abcam) and IdU (#347580 ...
-
bioRxiv - Cancer Biology 2023Quote: ... at rt for 1 h and blotted with the following primary antibodies at 4°C overnight: β-Actin (Abcam, ab8224), MDR1/ABCB1 (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibody staining was done sequentially in PBS/1% BSA at RT: rabbit anti-GFP (1:500, 1 h, Abcam, 6556), goat anti-rabbit IgG conjugated to 18 nm gold (1:20 ...
-
bioRxiv - Genomics 2023Quote: ... Cells were then incubated with the primary antibody (diluted in 0.5% BSA/PBS) at RT for 1h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1,000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1,000 ...
-
bioRxiv - Cancer Biology 2024Quote: ... incubated with the following primary antibody diluted in PBS-Tween (0.1%) for 1 h at RT: anti-Olig2 (1:100, Abcam, #ab109186), anti-Cleaved caspase 3 (1:100 ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were washed with PTX for 3 × 10 min at RT and incubated with secondary antibodies in PTX (1:200 goat anti-chicken IgY H&L DyLight 488 (Abcam)) for 2 h at RT ...
-
bioRxiv - Biophysics 2022Quote: ... Mycoplasma contamination was ruled out by PCR (Abcam 289834).
-
bioRxiv - Cell Biology 2020Quote: ... the following reagents were used for indicated periods of time – (1) SCD inhibitor (Abcam, ab142089) – 4 μM in DMSO (2 ...
-
bioRxiv - Physiology 2020Quote: ... Tissue was rinsed 3 times with PBST before secondary was applied (Goat antirabbit 647, Abcam) for 2 hours at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed 3 times with PBS and incubated in 3,3ʹ-Diaminobenzidine (DAB) chromogen (Abcam).Slides were then dehydrated in increasing grades of alcohol (70% ...
-
bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were stained with a 1:10 dilution of individual animal serum followed by detection with a 1:1000 dilution of a goat anti-mouse Fc FITC secondary (Abcam ab98716) and fixation with 4% formalin ...
-
bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
-
bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were pelleted and washed with PBS twice and then co-incubated in detection antibody (donkey anti rabbit IgG-AF488: Abcam[Ab150093])) diluted 1:5000 in PBS and incubated for 15 minutes at 4 degrees ...
-
bioRxiv - Biochemistry 2023Quote: ... Nucleosomes bound were detected using western blot followed by detection with histone antibodies (anti-H3 Abcam ab1791; anti-H2A Abcam ab18255, anti-H2B Abcam ab1790) and HRP conjugated secondary antibodies (anti-mouse IgG-HRP Vector Labs PI2000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
-
bioRxiv - Microbiology 2020Quote: ... Cloned IRE1-/- MEF cells were validated by RT-qPCR using specific primers and by immunoblot using IRE1-specific antibody (Abcam-ab37073). IRE1-/- cells were complemented with pcDNA3-derived vectors containing WT or the indicated mutant versions of IRE1 ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were rehydrated in PBS 3 times for 10 minutes and blocked with 7% normal goat serum in PBS for 1 hour and then incubated overnight at room temperature (RT) with anti-cFos (1/200, rabbit, Abcam ab209794) or anti-Cleaved-Caspase 3 (1/400 ...
-
bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... 1% goat serum with Triton was used as a blocking solution for at least 1 hour at RT and a Goat Anti-Rat IgG H&L (HRP) (Abcam ab97057) was used as a secondary and incubated for 1.5 hours at RT before continuing with the described DAB staining ...
-
bioRxiv - Molecular Biology 2019Quote: ... coverslips were blocked with 4% BSA/PBS for 45 min at RT and primary antibody anti-rabbit lamin B1 (abcam ab16048), mab414 (1:500 ...
-
bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes of N2a cells samples were incubated for 1 hour at room temperature (RT) with primary antibodies for ATP5A (1:1000, Abcam Ab119688) and GAPDH (1:2000 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were washed in 0.1% Triton X-100/TBS 3 times and incubated in secondary antibody and phalloidin for 1 h at room temperature (RT) (in Abdil, 1:2000 Alexa Fluor 488 Donkey Anti-Goat (Abcam ab150129) and 1:250 Phalloidin Atto 647N (Sigma-Aldrich 65906) ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... for 1 hr at RT and incubated in PBST overnight at 4°C with following primary antibodies: chicken anti-GFP (1:1000, Abcam ab13970), chicken anti-MAP2 (1:10000 ...
-
bioRxiv - Cell Biology 2019Quote: ... Same experiment was also performed blocking monocyte-derived macrophages at RT for 15 min with 10 μg/ml of mAb α-Siglec-1 7–239 (Abcam), IgG1 isotype control (BD Biosciences ...
-
bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
-
bioRxiv - Cell Biology 2019Quote: ... at RT for 1 h and incubated with the following primary antibodies overnight at 4°C: SUN2 (1:100; rabbit; Abcam ab124916), total β1-integrin (1:50 ...
-
bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were then treated with the blocking solution (10% Goat serum) for 1 h at RT and incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
-
bioRxiv - Immunology 2023Quote: ... was incubated with the transferred proteins for 2 h and the binding was detected after incubation for 1 h at RT with HRP-conjugated anti-HA antibody diluted 1:5000 (Abcam, Ab1190). The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... preferably filtered) was added for 15 min at RT before primary rb-anti-TMC3 (ATLAS HPA040265) and ms-anti-Beta3TUB (ABCAM, ab7751) antibody was added at 1:200 and VG cells were incubated at 4° C O/N on a lightly tilting tissue culture platform ...
-
bioRxiv - Biochemistry 2023Quote: ... 1% BSA in 0.5% PBST) for 1h at RT prior to the addition of primary antibodies (isoDGR [1:200] and CD68 [1:200, Abcam, ab283654]) overnight at 4 °C ...
-
Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... blocked in 1% bovine serum albumin in PBS for 1 h at RT and incubated with anti-pERM antibody (Abcam, ab76247), anti-alpha-tubulin-FITC (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
-
bioRxiv - Immunology 2023Quote: ... Cells were blocked for 30 min with 1% BSA previous 1h incubation at RT with the primary antibody rabbit anti-pericentrin (Abcam, ab4448), rabbit anti-pericentrin (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
-
bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
-
bioRxiv - Microbiology 2024Quote: ... A commercial kit (Immunoprecipitation Kit, Abcam), which provided buffers for protein extraction and solubilization as well as Protein A/G sepharose beads and washing buffers ...
-
bioRxiv - Immunology 2021Quote: ... the plates were washed 3 times with PBST and goat anti-pig IgG HRP antibody (Abcam) diluted 1:10,000 in PBST containing 1% FBS was added to the well (60 μl/well) ...
-
bioRxiv - Cell Biology 2022Quote: ... Potassium green (KG, 10μM, ION Potassium-Green-2 AM, K+ indicator, abcam ab142806; 45min incubation time), a lipophilic styryl dye (5μg/mL ...
-
bioRxiv - Cancer Biology 2022Quote: ... slides were washed two times with ddH2O and counterstained using hematoxylin solution (Abcam cat. no. ab220365) or counterstain supplied in kit for 1-2 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times in PBST and incubated in 1μM DAPI solution (#ab228549, Abcam) for 5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were washed in 1xPBST three times (5mins) and mounted with Fluoroshield with DAPI (Abcam, ab104139). Images were taken using the Leica DM7200 fluorescence imaging platform with LAS X version 3.7.5 ...