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Citations for abcam :
101 - 150 of 156 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
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bioRxiv - Cell Biology 2019Quote: ... at RT for 1 h and incubated with the following primary antibodies overnight at 4°C: SUN2 (1:100; rabbit; Abcam ab124916), total β1-integrin (1:50 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then treated with the blocking solution (10% Goat serum) for 1 h at RT and incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Immunology 2023Quote: ... was incubated with the transferred proteins for 2 h and the binding was detected after incubation for 1 h at RT with HRP-conjugated anti-HA antibody diluted 1:5000 (Abcam, Ab1190). The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... preferably filtered) was added for 15 min at RT before primary rb-anti-TMC3 (ATLAS HPA040265) and ms-anti-Beta3TUB (ABCAM, ab7751) antibody was added at 1:200 and VG cells were incubated at 4° C O/N on a lightly tilting tissue culture platform ...
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bioRxiv - Biochemistry 2023Quote: ... 1% BSA in 0.5% PBST) for 1h at RT prior to the addition of primary antibodies (isoDGR [1:200] and CD68 [1:200, Abcam, ab283654]) overnight at 4 °C ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... blocked in 1% bovine serum albumin in PBS for 1 h at RT and incubated with anti-pERM antibody (Abcam, ab76247), anti-alpha-tubulin-FITC (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked for 30 min with 1% BSA previous 1h incubation at RT with the primary antibody rabbit anti-pericentrin (Abcam, ab4448), rabbit anti-pericentrin (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... from TSA kit for 10-15’ at RT followed by incubation with goat anti-rabbit 568 (1:500, ab175471, Abcam, Cambridge, UK) in TNB blocking buffer and DAPI (1:1000 in 1x PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated under darkness at RT with a goat anti-mouse secondary antibody (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Cell Biology 2020Quote: ... slides were blocked with PBS + 1% BSA + 2% goat serum + 0.2% Triton X-100 for 1h at RT and incubated overnight at 4◦C with anti-DCLK1 antibody (Abcam #ab31704; 1:250) in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed in 1X PBS and then incubated in a blocking solution containing 3% normal goat serum and 0.3% triton X-100 in 1X PBS for 60 mins at RT prior to incubation with Laminin (Abcam #ab11575; 1:200) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 2.5 hours at RT using two different anti-BrdU antibodies recognizing CldU (Abcam, ab6326, 1:500) and IdU (Becton Dickinson ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently incubated for 1h at RT with the secondary antibody diluted in the blocking buffer solution: Donkey anti Rabbit 647 (1:1000, AB150075, ABCAM, Cambridge, UK); Donkey anti Mouse 594 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Immunology 2023Quote: ... The slides were then blocked with 10% normal goat serum phosphate-buffered saline (PBS) for 30 min at room temperature (RT) and then were incubated with either Hamster anti-PDPN (Abcam Cat#: ab11936) or Rabbit anti-proSP-C (Millipore Sigma Cat# ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Immunology 2020Quote: ... Genotyping PCR products were digested with AvaI restriction enzyme (Abcam, Cambridge, Massachusetts) for 1 hour at 37°C and electrophoresed on a 3% agarose gel in 1X Tris-acetate-EDTA (TAE ...
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bioRxiv - Biochemistry 2022Quote: ... and re-probed (1h, RT, 1/10000 diluted in PBS-Tween with 5% non-fat milk) with rat anti-β actin (AbCam, Cambridge, UK, RRID: AB_867488) washed (as above ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed 3-times with PBS and stained with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Abcam, ab150077; RT, 1 h) to detect Aβ ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed 3 times with PBS and stained with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Abcam, ab150077; RT, 1 h) to detect Aβ ...
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bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.05% Triton X-100 in PBS) was performed for 1 hour at RT followed by primary Ab (ab237715, Abcam, diluted 1:1000, 0.1% FBS in PBS) incubation for 1 hour at RT ...
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bioRxiv - Genomics 2023Quote: ... Cells were regularly tested for mycoplasma contamination using the Mycoplasma PCR Detection Kit (abcam ab289834). E14 mouse embryonic stem cells were a gift from W ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic PCR and western blot (anti-DUS1L, Proteintech, 1:1000; anti-DUS2L, Abcam, 1:1000) were performed to confirm KO.