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Citations for abcam :
51 - 100 of 157 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... permeabilized in 0,02% Triton X-100 at RT for 15 minutes and incubated with anti-HA antibody (1:1000, #9110, Abcam) in staining buffer at RT for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated for 1h at RT with goat anti-mouse IgG conjugated with Alexa Fluor-555 (Abcam, UK) (1:500 dilution in blocking buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... The antibodies used for western blotting was anti-cltc (1:1000 in 0.1% Tween20, 0.05% milk in PBS, RT 1h, Abcam ab21679) and anti-GAPDH (1:10000 in TBST ...
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bioRxiv - Cell Biology 2022Quote: ... diluted in blocking buffer for one hour at RT: donkey anti-rat Alexa Fluor® 647 (1:250; abcam); donkey anti-goat Alexa Fluor® 568 (1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The newly replicated CldU and IdU tracks were stained for 2.5 hours at RT using two different anti-BrdU antibodies recognizing CldU (#ab6326, Abcam) and IdU (#347580 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... blots were incubated for 1 h at RT with Rat HRP-conjugated α-mouse (1:10,000, Abcam mAb 131368). To analyze the co-immunoprecipitations ...
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bioRxiv - Microbiology 2023Quote: ... The tilter of the sgRNA lentiviral library was determined by RT-qPCR using the qPCR Lentivirus Titer Kit (Abcam) following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... samples were washed and incubated for 1 hr at RT with Anti-HA Rb Ab (1:500, Abcam, ab9110) in blocking buffer ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Duke University] and GAPDH (mouse anti-human GAPDH, 1:10,000, 1 hr at RT; Cat: ab8245, Abcam, Waltham, MA). Primary antibodies were detected using donkey anti-rabbit IRDye® 800CW IgG (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody mouse anti-Nup153 (Abcam) at 1:250 for 30 m ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then washed the 3 times in 1XPBS 5min at RT and incubated with the secondary antibody (1:200, Abcam ab175471) for 2-3h at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated at RT for 1 hour with primary antibodies diluted in PBS containing 1% FBS (CHC (Abcam, ab21679), GM130 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked with 5% bovine serum albumin (BSA) in PBS for 1 hour at RT and incubated with primary rabbit anti-collagen IV antibody (1:100) (Abcam) in 1% BSA in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5% BSA in PBS for 1 h at RT and incubated with primary antibodies 1:500 Anti-p53 (9.1) mouse mAb (Abcam, #ab77813) overnight at 4°C and 1:1000 Anti-GAPDH (14C10 ...
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bioRxiv - Cell Biology 2021Quote: ... Sirius red was performed on deparaffinized liver sections that were incubated for 1h at RT in Picro Sirius Red solution (ab150681, Abcam), followed by washes in acetic acid and alcohol solutions.
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1hr at RT with a horseradish peroxidase-conjugated anti-rabbit goat serum (1:10000; Abcam ab6721). After washing ...
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bioRxiv - Cell Biology 2020Quote: ... gels were incubated 24 hours at RT on a rocking platform with rabbit anti-TOM20 antibody (Abcam, catalog no. ab78547). Gels were then washed in PBS-T (0.1% Tween 20 in 1× PBS ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 min at RT and then overnight at 4 °C in a humidified chamber with the following antibodies: LC3 (1:50, Abcam), p62 (1:20 ...
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated with 3% BSA and 0.1% Triton X-100 for 30 min at RT and stained with anti-gamma H2AX (Abcam 2893), anti CENP B (Santa Cruz ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently incubated for 1h at RT with Alexa-488-coupled anti-LAMP1 antibody (1:500 in PBS containing 1% BSA, Abcam). Cells were washed twice in PBS and stored at 4°C in the dark.
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bioRxiv - Cancer Biology 2020Quote: ... The dishes were then incubated with blocking buffer ar RT for 1 hr and immediately the primary antibody (Coilin, ab87913; PML, ab96051; RNAPII, ab193468; Abcam) diluted in antibody diluent was applied and incubated at RT for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in blocking solution containing primary antibodies for 1 hr at RT (EnCOR Chicken-anti-GFAP at 1:2000, Abcam Rabbit-anti-pRab10 at 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450 ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Genetics 2020Quote: ... Incubation of the secondary antibody was performed for 1h at RT with either rabbit anti-mouse (abcam # ab46540, for H3K27me3) or guinea pig anti-rabbit antibody(Antibodies online #ABIN101961 ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... Brain slices were then incubated for ∼20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry ...
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bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 3h at RT using two different anti-BrdU antibodies recognizing CldU (#ab6326, Abcam) and IdU (#347580 ...
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bioRxiv - Cancer Biology 2023Quote: ... at rt for 1 h and blotted with the following primary antibodies at 4°C overnight: β-Actin (Abcam, ab8224), MDR1/ABCB1 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody staining was done sequentially in PBS/1% BSA at RT: rabbit anti-GFP (1:500, 1 h, Abcam, 6556), goat anti-rabbit IgG conjugated to 18 nm gold (1:20 ...
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bioRxiv - Genomics 2023Quote: ... Cells were then incubated with the primary antibody (diluted in 0.5% BSA/PBS) at RT for 1h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1,000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1,000 ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with the following primary antibody diluted in PBS-Tween (0.1%) for 1 h at RT: anti-Olig2 (1:100, Abcam, #ab109186), anti-Cleaved caspase 3 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed with PTX for 3 × 10 min at RT and incubated with secondary antibodies in PTX (1:200 goat anti-chicken IgY H&L DyLight 488 (Abcam)) for 2 h at RT ...
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bioRxiv - Biophysics 2022Quote: ... Mycoplasma contamination was ruled out by PCR (Abcam 289834).
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rehydrated in PBS 3 times for 10 minutes and blocked with 7% normal goat serum in PBS for 1 hour and then incubated overnight at room temperature (RT) with anti-cFos (1/200, rabbit, Abcam ab209794) or anti-Cleaved-Caspase 3 (1/400 ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2019Quote: ... 1% goat serum with Triton was used as a blocking solution for at least 1 hour at RT and a Goat Anti-Rat IgG H&L (HRP) (Abcam ab97057) was used as a secondary and incubated for 1.5 hours at RT before continuing with the described DAB staining ...
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bioRxiv - Molecular Biology 2019Quote: ... coverslips were blocked with 4% BSA/PBS for 45 min at RT and primary antibody anti-rabbit lamin B1 (abcam ab16048), mab414 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of N2a cells samples were incubated for 1 hour at room temperature (RT) with primary antibodies for ATP5A (1:1000, Abcam Ab119688) and GAPDH (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 0.1% Triton X-100/TBS 3 times and incubated in secondary antibody and phalloidin for 1 h at room temperature (RT) (in Abdil, 1:2000 Alexa Fluor 488 Donkey Anti-Goat (Abcam ab150129) and 1:250 Phalloidin Atto 647N (Sigma-Aldrich 65906) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hr at RT and incubated in PBST overnight at 4°C with following primary antibodies: chicken anti-GFP (1:1000, Abcam ab13970), chicken anti-MAP2 (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by washing in PBS (3 × 15 min) and an overnight incubation (>16 hr at RT) with the primary antibody (1:1000, rat anti-Ctip2, Abcam #ab18465) diluted in antibody solution (1% goat serum and 0.2% Triton X-100 in PBS) ...