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Citations for abcam :
401 - 450 of 2151 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Microbiology 2020Quote: ... Cloned IRE1-/- MEF cells were validated by RT-qPCR using specific primers and by immunoblot using IRE1-specific antibody (Abcam-ab37073). IRE1-/- cells were complemented with pcDNA3-derived vectors containing WT or the indicated mutant versions of IRE1 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rehydrated in PBS 3 times for 10 minutes and blocked with 7% normal goat serum in PBS for 1 hour and then incubated overnight at room temperature (RT) with anti-cFos (1/200, rabbit, Abcam ab209794) or anti-Cleaved-Caspase 3 (1/400 ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076; donkey anti-goat IgH&L Alexa488, Abcam, ab150129). Tissues were washed in 1% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2019Quote: ... 1% goat serum with Triton was used as a blocking solution for at least 1 hour at RT and a Goat Anti-Rat IgG H&L (HRP) (Abcam ab97057) was used as a secondary and incubated for 1.5 hours at RT before continuing with the described DAB staining ...
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bioRxiv - Molecular Biology 2019Quote: ... coverslips were blocked with 4% BSA/PBS for 45 min at RT and primary antibody anti-rabbit lamin B1 (abcam ab16048), mab414 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of N2a cells samples were incubated for 1 hour at room temperature (RT) with primary antibodies for ATP5A (1:1000, Abcam Ab119688) and GAPDH (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 0.1% Triton X-100/TBS 3 times and incubated in secondary antibody and phalloidin for 1 h at room temperature (RT) (in Abdil, 1:2000 Alexa Fluor 488 Donkey Anti-Goat (Abcam ab150129) and 1:250 Phalloidin Atto 647N (Sigma-Aldrich 65906) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 hr at RT and incubated in PBST overnight at 4°C with following primary antibodies: chicken anti-GFP (1:1000, Abcam ab13970), chicken anti-MAP2 (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by washing in PBS (3 × 15 min) and an overnight incubation (>16 hr at RT) with the primary antibody (1:1000, rat anti-Ctip2, Abcam #ab18465) diluted in antibody solution (1% goat serum and 0.2% Triton X-100 in PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Same experiment was also performed blocking monocyte-derived macrophages at RT for 15 min with 10 μg/ml of mAb α-Siglec-1 7–239 (Abcam), IgG1 isotype control (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
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bioRxiv - Cell Biology 2019Quote: ... at RT for 1 h and incubated with the following primary antibodies overnight at 4°C: SUN2 (1:100; rabbit; Abcam ab124916), total β1-integrin (1:50 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then treated with the blocking solution (10% Goat serum) for 1 h at RT and incubated with the primary antibodies for TNNT2 (Abcam, ab45932), Ki67 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Immunology 2023Quote: ... was incubated with the transferred proteins for 2 h and the binding was detected after incubation for 1 h at RT with HRP-conjugated anti-HA antibody diluted 1:5000 (Abcam, Ab1190). The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... preferably filtered) was added for 15 min at RT before primary rb-anti-TMC3 (ATLAS HPA040265) and ms-anti-Beta3TUB (ABCAM, ab7751) antibody was added at 1:200 and VG cells were incubated at 4° C O/N on a lightly tilting tissue culture platform ...
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bioRxiv - Biochemistry 2023Quote: ... 1% BSA in 0.5% PBST) for 1h at RT prior to the addition of primary antibodies (isoDGR [1:200] and CD68 [1:200, Abcam, ab283654]) overnight at 4 °C ...
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Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopybioRxiv - Bioengineering 2023Quote: ... blocked in 1% bovine serum albumin in PBS for 1 h at RT and incubated with anti-pERM antibody (Abcam, ab76247), anti-alpha-tubulin-FITC (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked for 30 min with 1% BSA previous 1h incubation at RT with the primary antibody rabbit anti-pericentrin (Abcam, ab4448), rabbit anti-pericentrin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... A commercial kit (Immunoprecipitation Kit, Abcam), which provided buffers for protein extraction and solubilization as well as Protein A/G sepharose beads and washing buffers ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 3 times with PBST and goat anti-pig IgG HRP antibody (Abcam) diluted 1:10,000 in PBST containing 1% FBS was added to the well (60 μl/well) ...
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bioRxiv - Cell Biology 2022Quote: ... Potassium green (KG, 10μM, ION Potassium-Green-2 AM, K+ indicator, abcam ab142806; 45min incubation time), a lipophilic styryl dye (5μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed two times with ddH2O and counterstained using hematoxylin solution (Abcam cat. no. ab220365) or counterstain supplied in kit for 1-2 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times in PBST and incubated in 1μM DAPI solution (#ab228549, Abcam) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed in 1xPBST three times (5mins) and mounted with Fluoroshield with DAPI (Abcam, ab104139). Images were taken using the Leica DM7200 fluorescence imaging platform with LAS X version 3.7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Horseradish peroxidase-labeled conformation-sensitive secondary anti-mouse IgG for IP or anti-rabbit VeriBlot for IP Detection antibody (both at 1:20000; Abcam, Cambridge, MA) was detected by SuperSignal West Femto Maximum Sensitivity substrate (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... three pre-diluted primary antibodies were sequentially applied in the following order using the indicated chromogenic detection: anti-CASQ1 (1:10000, Abcam # ab185220, Abcam, Cambridge, UK) with Omap anti-Ms HRP (Ventana Discovery #760-4310 ...
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bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and a polyclonal rabbit serum against SARS-CoV-2 spike was added and incubated for 1 hour following a wash step and detection of binding with goat anti rabbit-HRP (Abcam catalog # ab97080). TMB substrate was added to the plates for detection of HRP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated under darkness at RT with a goat anti-mouse secondary antibody (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Cell Biology 2020Quote: ... slides were blocked with PBS + 1% BSA + 2% goat serum + 0.2% Triton X-100 for 1h at RT and incubated overnight at 4◦C with anti-DCLK1 antibody (Abcam #ab31704; 1:250) in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed in 1X PBS and then incubated in a blocking solution containing 3% normal goat serum and 0.3% triton X-100 in 1X PBS for 60 mins at RT prior to incubation with Laminin (Abcam #ab11575; 1:200) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Cancer Biology 2023Quote: ... The newly replicated CldU and IdU tracks were stained for 2.5 hours at RT using two different anti-BrdU antibodies recognizing CldU (Abcam, ab6326, 1:500) and IdU (Becton Dickinson ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently incubated for 1h at RT with the secondary antibody diluted in the blocking buffer solution: Donkey anti Rabbit 647 (1:1000, AB150075, ABCAM, Cambridge, UK); Donkey anti Mouse 594 ...
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bioRxiv - Immunology 2023Quote: ... The slides were then blocked with 10% normal goat serum phosphate-buffered saline (PBS) for 30 min at room temperature (RT) and then were incubated with either Hamster anti-PDPN (Abcam Cat#: ab11936) or Rabbit anti-proSP-C (Millipore Sigma Cat# ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... the Catalase kit buffer (Abcam, kit ab83464) for catalase activity ...