Labshake search
Citations for abcam :
1 - 50 of 1655 citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... anti-TIM-3 (Abcam, catalog #ab241332), and anti-PD-1 (Cell Signaling Technology ...
-
bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
-
bioRxiv - Physiology 2021Quote: ... HEK-293 cells were cultured and treated with 2 µg/mL of tunicamycin (ab120296, Abcam) for 6 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
bioRxiv - Immunology 2022Quote: ... 3 ml of the adjuvant goat IgG anti-human IgM Fc (5 ng/ml) (by ABCAM, Cambridge, UK) was dissolved in human albumin and phosphate-buffered normal saline added to the remainder of the sediments and packaged in 1 ml vials of injectable solution with labels (See Supplement ...
-
bioRxiv - Bioengineering 2019Quote: Extracellular domain Fc fusions for human CTLA4 (CTLA4-Fc) and ErbB2 (ErbB2-Fc) were purchased from Abcam (ab180054 and ab168896). These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... CoMiX-Fc were detected with a goat anti-human IgG Fc HRP pAb (Abcam, #ab97225), whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... Commercially obtained human CTSL (Cathepsin L/MEP) knockout HEK-293T cells (abcam; ab266521) and human wild-type HEK293T cells (abcam ...
-
bioRxiv - Neuroscience 2021Quote: ... On DIV14 cells were treated with human Fc (Abcam #90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
-
bioRxiv - Immunology 2020Quote: ... HRP conjugated goat anti-human IgG Fc was from Abcam, Cambridge ...
-
bioRxiv - Microbiology 2023Quote: The Fc portion of human Immunoglobulin G (1 mg, Abcam) was labelled with biotin (Thermo Scientific EZ-Link Sulfo-NHS-Biotin ...
-
bioRxiv - Neuroscience 2023Quote: ... cultures were treated with purified Fc from human IgG (Abcam #ab90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
-
bioRxiv - Cell Biology 2021Quote: ... bound IRE1α LD-Fc was transferred after washes to a PVDF membrane and detected with anti-human Fc antibody (ab977225, Abcam), ECL solution (Thermo-Fisher ...
-
bioRxiv - Immunology 2024Quote: ... 100 ng/well goat anti-human IgG Fc (Abcam, Cambridge, UK, #ab97221) or rabbit anti-His (Bethyl ...
-
bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
-
bioRxiv - Bioengineering 2021Quote: ... anti-human cleaved Caspase 3 (Abcam #ab2302). Biotinylated lotus tetranoglobus lectin was purchased from Fisher Scientific (#NC0370187 ...
-
bioRxiv - Immunology 2021Quote: ... the antigens and a capture anti-human IgE (Mouse anti-Human IgE Fc) (ref. ab99834, Abcam PLC, Cambridge, UK) were coupled to magnetic MagPlex® 6.5μm COOH-microspheres (Luminex Corporation ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera) (ab272105, Abcam) was used as positive control (loaded 2.4 ug/lane) ...
-
bioRxiv - Neuroscience 2020Quote: ... ephrin-A5 conjugated to human FC (ephrinA5-hFC, R&D) or hFC control (R&D) was pre-clustered with goat anti- human antibody (Abcam) for one hour at 37°C before being added to transfected HEK cells (5 μg /ml ...
-
bioRxiv - Immunology 2024Quote: ... CoMiX-Fc molecules were revealed with goat anti-human IgG AF647-conjugated antibody (Abcam), which cross-reacts with mouse IgGs ...
-
bioRxiv - Immunology 2020Quote: ... followed by incubation with PE-labeled goat anti-human IgG-Fc antibody (1:5000; Abcam) for 30 min and analyzed by flow cytometry.
-
bioRxiv - Microbiology 2022Quote: ... cells were dissociated and incubated with recombinant human ACE2-Fc(IgG) (1 mg/ml) (Abcam ab273687) at a dilution of 1:32000 for 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... and directly incubated with the secondary antibody using Goat Anti-Human IgG Fc (HRP) preadsorbed (Abcam). After washing five times with Tris-buffered saline and Tween 20 ...
-
bioRxiv - Bioengineering 2021Quote: ... anti-human cleaved Caspase 3 was purchased from Abcam (ab2302). Primary antibodies were detected with peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:3000 ...
-
bioRxiv - Immunology 2020Quote: ... or anti-his-APC and anti-his-PE antibodies (Abcam) to target the His tag of RBD ...
-
bioRxiv - Immunology 2023Quote: ... Tissues stained with Tim-4 were incubated overnight at 4°C with rabbit anti-IBA1 (ab178846, abcam), followed by anti-rabbit AF488 secondary antibody or with rabbit anti-IBA1 AF647 (ab225261 ...
-
bioRxiv - Biochemistry 2020Quote: ... His (Abcam, ab18184), H3K36me3 (CST ...
-
bioRxiv - Immunology 2020Quote: ... after twice washes then PE-labeled goat anti-human IgG-Fc antibody was added (1:5,000; Abcam) for 30 min ...
-
bioRxiv - Microbiology 2023Quote: ... The secondary antibodies included: (1) goat anti-human IgG Fc (HRP) pre-adsorbed (catalog no. ab98624; Abcam); (2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
-
bioRxiv - Cell Biology 2021Quote: ... Plates were washed again with PBSt and incubated with an anti-human Fc HRP-conjugated antibody (ab977225, Abcam). Development was performed with TMB solution and terminated with Stop solution (Biolegend) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... Supernatants were then analyzed for galectin 3 content using a human galectin 3 ELISA kit (Abcam, ab 188394) as recommended by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: ... and C3 levels by Human Complement 3 ELISA kit (Abcam, Cambridge, MA) as per manufacturer instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... and HIS-tag (Abcam) were used ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-His (ab18184, abcam); anti-β-actin (A1978 ...
-
bioRxiv - Cell Biology 2019Quote: ... rabbit His (Abcam ab9108), rabbit Ndc80 (82) ...
-
bioRxiv - Immunology 2023Quote: ... or anti-His (Abcam) were added to the samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-His (Abcam, #ab18184), anti-MYC (A190– 205A) ...
-
bioRxiv - Cell Biology 2022Quote: ... The assays were carried out in 50 μl mTOR reaction buffer with or without 200 μM ATP and/or the recombinant human His-tagged ATF4 (Cat. ab109946, Abcam) at 30°C for 45 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... or Fc-iNKG2D.AF and bound MicAbody detected with an HRP-conjugated mouse-anti-human kappa chain antibody (Abcam #ab79115). Affinity of both monomeric and antibody-fused ULBP2 variants was also determined by Octet analysis as described above.
-
bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
-
bioRxiv - Biochemistry 2023Quote: ... His-GST-tagged USP7 (SinoBiological 11681-H20B) and His-tagged GST (Abcam ab89494) were immobilized to the streptavidin sensor chip (Cytiva BR100531 ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 µL of mouse polyclonal anti-HIS H8 to 6X His Tag (Abcam) or 5 µL of IgG from mouse serum as negative control ...
-
bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
-
bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
-
bioRxiv - Cell Biology 2019Quote: ... and anti-His (ab9108; Abcam), respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... α-His (Abcam, NB100-63173), mouse α-CPY (Invitrogen 10A5B5).
-
bioRxiv - Cell Biology 2021Quote: ... anti-His (1:1000) (Abcam) was used ...
-
bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).