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Citations for abcam :
351 - 400 of 436 citations for Semaphorin 3E Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Blocking solution was removed and replaced with blocking solution containing primary antibodies against dsRNA (Scicons, Szirák, Hungary) and alpha tubulin (Abcam, Cambridge, UK) (both diluted 1:1000) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 15 minutes then blocked with 1% bovine serum albumin in PBSTx (blocking buffer) and immunostained with antibodies against NPM1 (Abcam, ab10530) and gH2A.X pSer139 (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then incubated at 4°C overnight in blocking buffer with the following antibodies and concentrations: chicken anti-GFP (1:1000; Abcam ab13970), rabbit anti-per (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were washed three times with PBS following fixation and blocked with blocking buffer: 5% normal goat serum (NGS, Abcam, AB7681) in PBS with 0.1% Triton-X (PBST) ...
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bioRxiv - Developmental Biology 2024Quote: ... Erythrocytes were incubated for 30 min at room temperature in blocking buffer and stained directly on slides with anti-RNAP II Phosho-Ser2 (Abcam #5095) in the blocking buffer (1X PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... membranes were incubated overnight with a peptide specific rabbit anti-IMP1 antibody (Nielsen et al., 1999) an anti-YBX1 antibody (ab12148, Abcam), an anti-GFP antibody (ab1218 ...
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bioRxiv - Bioengineering 2019Quote: Levels of brain natriuretic peptide (BNP) secreted in the media were quantified by Human BNP ELISA Kit (Abcam, cat# ab193694) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Blots were washed again in 3X TBS-T then incubated in blocking buffer containing a 1:5000 dilution of rabbit Anti-DDDDK HRP conjugated antibody (Abcam Cat #ab2493). Blots were washed three times with TBST and once with TBS ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were diluted in blocking solution and used overnight at 4°C: chicken anti-GFP (#13970, Abcam; 1:2000), rabbit anti-dsRed (#632496 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with appropriate primary antibodies prepared in blocking buffer against glucagon (mouse antiglucagon antibody, Cat # ab10988, Abcam; 1:1000 or rabbit anti-glucagon antibody, Cat# ab92517, Abcam; 1:25), Stathmin-2 (goat anti-SCG10 antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4 °C with primary antibodies prepared in blocking buffer against phospho-MLKL (Ser345) (Abcam, Cat. No. ab196436), MLKL (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing chicken anti-GFP polyclonal antibody (1:1000; Abcam, Cambridge, MA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then incubated overnight in humidified chambers at 4°C in the following primary antibodies diluted in blocking buffer: anti-MAP2 (1:700; Ab5392, Abcam, Cambridge, UK), SMI312 (1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in rabbit anti-c-Fos polyclonal antibody (1:1000 in blocking buffer; catalog no. 190289, Abcam, Cambridge, United Kingdom) for 48 hr at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots (2 µg per lane) were probed with primary antibodies specific for CD68 (ab213363, 1/500 in blocking buffer; Abcam, Cambridge, UK) or β-actin (4967S ...
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bioRxiv - Cancer Biology 2019Quote: ... after pretreatment with Vector Labs Mouse on Mouse Blocking Reagent (MKB-2213) according to manufacturer’s instructions and anti-LAMININ (Abcam, ab11576, 1:1000).
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 24 hr in blocking buffer containing a chicken anti-mCherry antibody (1:500, ab205402, Abcam, Cambridge, MA). After PBS washes ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary antibodies diluted in blocking buffer over night at 4°C (SHIP1, 1:500 dilution, Cell Signaling Technologies; IBA1, 1:500 dilution, abcam; GAPDH ...
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bioRxiv - Physiology 2022Quote: ... primary antibodies were added in 50 μL blocking buffer for 12 hours at 4° C: ATP synthase antibody (Abcam, ab128743 1:200), sAC antibody (R21 IHC ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... mitochondria were immunostained in Intercept Blocking Buffer with 0.2% Triton X-100 and Alexa Fluor 647 Anti-TOMM20 antibody (Abcam, ab209606, 1:100) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently incubated for 1h at RT with the secondary antibody diluted in the blocking buffer solution: Donkey anti Rabbit 647 (1:1000, AB150075, ABCAM, Cambridge, UK); Donkey anti Mouse 594 ...
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bioRxiv - Bioengineering 2023Quote: ... we replaced the blocking buffer with a fresh 250uL of the same blocking buffer containing a 1:1000 dilution of our primary antibody against ACE2 (polyclonal Rabbit-anti-hACE2, Abcam, Cat# Ab15348). Following a 1 hour incubation at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing chicken anti-GFP polyclonal antibody (1:1000; Abcam, Cambridge, MA) with gentle agitation at 4°C for 18–22 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam, Cambridge, MA) for 90 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-glucagon (ABCAM; ab82270) primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were incubated over night at 4°C in blocking buffer containing rabbit anti-β III Tubulin (1:1000, Abcam, Cambridge, UK) for neurons detection ...
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bioRxiv - Cell Biology 2019Quote: ... the sections were incubated with blocking solution (Beyotime, P0102) for one hour at room temperature and incubated with primary antibody against BrdU (Abcam, ab6326, 1:100) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were then permeabilized with 0.2% Triton X for 30 minutes at room temperature followed by blocking with 5% normal donkey serum (Abcam plc, Cambridge, MA, USA) and 300 mmol/L glycine for one hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and 50 μL Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were striped from anti N-VEGF or VEGF polyclonal antibodies and following re-blocking reacted with primary antibodies directed against Lactate Dehydrogenase (anti-LDH) (Abcam, cat #ab47010,1:300) and secondary antibodies anti-goat (1:10,000) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were incubated at 4°C overnight diluted as noted in Odyssey blocking buffer: rabbit monoclonal anti-Vinculin antibody (abcam, Cambridge, MA [EPR8185]) at 1:3000 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2-4 μg/ml in blocking buffer (PBS + 4% FBS) containing 1 μg/ml mouse anti-tubulin (Abcam, ab7291, Cambridge, UK). After washing with PBS ...
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bioRxiv - Genetics 2019Quote: ... Slides were immunostained overnight at 4°C or 4h at 30°Cwith a mixture of the two primary antisera diluted in blocking buffer [rat anti-tubulin (Abcam ab6160 1:1250) and rabbit anti-MYC (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... in phosphate buffered saline (PBS) prior to overnight incubation at 4°C with blocking buffer containing primary antibodies raised against HIS48 (Abcam, Ab33760, 1:20), CD68 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...