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Citations for abcam :
201 - 224 of 224 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The second replicate gel was probed with a 1:3,000 dilution of α-myc mouse monoclonal antibody (Abcam, ab32) and secondary 1:200,000 dilution of goat α-mouse HRP (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were washed once with distilled water and stained with InstantBlue® Coomassie Protein Stain (Abcam, Cambridge, UK). The gels were viewed using a ChemiDOC XRS molecular imager (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... The denatured gel was then transferred to PVDF membrane and incubated with anti-Proteasome 20S alpha antibody (Catalog No. ab22674, Abcam).
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bioRxiv - Molecular Biology 2021Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-Nop58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Cell Biology 2020Quote: ... gels were incubated 24 hours at RT on a rocking platform with rabbit anti-TOM20 antibody (Abcam, catalog no. ab78547). Gels were then washed in PBS-T (0.1% Tween 20 in 1× PBS ...
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bioRxiv - Physiology 2022Quote: ... Samples were prepared using non-reducing sample buffer and were loaded (20 ug/well) onto the gelatin gel along with recombinant MMP9 (Abcam) and MMP2 (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... The bead slurries used were c-myc-conjugated (EZview red rabbit anti-c-myc affinity gel, Sigma, or Agarose goat anti-myc tag antibody, Abcam) and ubiquitin binding domain (UBD)-conjugated matrix (Ubiqapture-Q ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Microbiology 2023Quote: The main primary antibodies used for gel analysis of virus-infected cells were a commercial HSV-1 VP16 antibody (Abcam) and a rabbit polyclonal antibody against the full-length PRV VP16 that was ordered from Genscript ...
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bioRxiv - Synthetic Biology 2024Quote: ... Percent purity of each sample was determined by running diluted aliquots of each purified protein on a 4-12% Bis-Tris gel and staining with Optiblot Blue (Abcam). After destaining ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins quantified using BSA standard curve run alongside the protein on a Bis-Tris gel and stained using InstantBlue Coomassie Protein Stain (ab119211, Abcam).
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bioRxiv - Neuroscience 2020Quote: ... Samples were resolved by SDS-PAGE using a 4-15% gradient gel and analyzed by Western blot using the following primary antibodies: rabbit anti-GFP (Abcam Ab290), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: chick anti-GFP (Abcam Ab13970), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Biochemistry 2021Quote: ... Known amounts of purified 6His-IQGAP1 were run on gels alongside U2OS cell lysates and blotted with a 1:1,000 dilution of rabbit anti-IQGAP1 (ab133490; Abcam, Cambridge MA). Blots were washed ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved by electrophoresis using NuPAGE Novex 4–20% Bis-Tris gels and detected by Western blotting with the indicated antibodies: rabbit anti-Histone H3 (1:10000, abcam; ab1791), mouse anti-PCNA (1:2000 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Plant Biology 2024Quote: ... The immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with anti-HA antibodies (Anti-HA High Affinity Roche 3F/10, Sigma-Aldrich, and anti-HA Abcam). At least three independent biological replicates were performed per experiment ...
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bioRxiv - Cell Biology 2020Quote: ... gels were transferred to PVDF membranes at 25 V in 4 °C overnight and incubated with ATP5a antibody (Abcam, ab14748, 1:3000), followed by anti-mouse secondary antibody.
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Bioengineering 2022Quote: To visualize fibronectin coupled to BP-PA gels alongside photopatterned polyT20F ssDNA (figure 1e) we first incubated the gels in a rabbit anti-fibronectin antibody (1:100, ab23750, Abcam, RRID: AB_447655) diluted in 1x DPBS for 45 minutes followed by donkey anti-rabbit IgG-AlexaFluor 647 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from harvested cells were extracted with RIPA-lysis buffer and 15-30µg total protein was separated using 10% denaturing polyacrylamide gel followed by transfer methanol activated-PVDF membrane and was probed with anti-c-Myc antibody (ab32072, Abcam, Cambridge, UK). The blots were developed in X-ray film using an ECL kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates were subjected to gel electrophoresis and transferred to membranes that were incubated with an antibody against AMPKα1 (Abcam cat. no. ab32047), DNMT1 (Cell Signaling cat ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...