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Citations for abcam :
451 - 500 of 1658 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The surface of the coverslip was activated by incubation with 20 µg/ml anti-S-tag antibodies (Abcam) diluted in BRB80 buffer for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... Immunodetections were performed with an anti-His antibody (anti-6X His tag® antibody ab18184, Abcam, 1:1,000). The anti-mouse antibody from sheep coupled to horseradish peroxidase was used as a secondary antibody (NA931 ...
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bioRxiv - Molecular Biology 2024Quote: ... the flow cell was incubated with a biotinylated anti-6X His tag antibody (cat #ab27025, Abcam, Cambridge, MA) (1 µg/mL ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2; ab196883) and monoclonal antibodies against phospho-ERK1/2 (p-ERK1/2; ab201015) were purchased from Abcam. Protein expression was normalized to GAPDH (sc-2577 ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50; rabbit anti-Tuj1, Abcam, ab18207 at 1:1000). Tissues were washed for 20 min ...
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bioRxiv - Immunology 2021Quote: ... 2-N-nitrobenz-2-oxa-1,3diazol-4-amino]-2 deoxy-D-glucose (2NBDG) (Abcam). CD4+ cells were cultured for 24h in anti-CD3 coated plates (0.5μg/ml ...
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bioRxiv - Immunology 2020Quote: ... after twice washes then PE-labeled goat anti-human IgG-Fc antibody was added (1:5,000; Abcam) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies included: (1) goat anti-human IgG Fc (HRP) pre-adsorbed (catalog no. ab98624; Abcam); (2 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-V5 tag (Thermo Fisher Scientific, R960-25; IB 1:5000, IP 1μg, and Abcam, Ab53418; IF 1:800), anti-GFP (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: The immunodetection of G5845-1508 proteins were performed with different antibodies depending on the protein tag (anti-CBP (Abcam) for G5845-1508-TAP ...
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bioRxiv - Microbiology 2022Quote: ... protein expression was determined by Western blotting using as primary antibodies anti-FLAG or anti-V5 tag antibody (AbCam; 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-trypsin antibody (Ab-200997) and mouse anti-his tag (Ab-49936) were obtained from Abcam (Cambridge, UK). Affi-gel 10 resin was obtained from Bio-Rad laboratories (Hercules ...
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bioRxiv - Microbiology 2019Quote: ... the samples were incubated at 4°C for 24 h with anti-V5 tag monoclonal antibody (ab27671, Abcam, UK) at a 1:20 dilution at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... ROR1 scFvs also contained a VSV-g tag that were detected by anti-VSV-g polyclonal antibody (FITC, Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... The identity of all truncated forms of eIF3g was confirmed by western blotting using antibodies against His6-tag (Abcam).
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bioRxiv - Microbiology 2023Quote: ... The blot was probed with an anti-His-tag antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cat No. ab1178). Alternatively ...
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bioRxiv - Biophysics 2024Quote: ... Successful overexpression of AGO2 was confirmed using Western Blot analysis with an anti-6X His tag antibody (Abcam, AB18184).
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bioRxiv - Immunology 2021Quote: ... Lymph node tissue sections were stained with human anti-spike antibody P2B-2F6 (72) (1:50) and biotinylated goat anti-human secondary antibody (Abcam, catalog no. ab7152, 1:300), followed by streptavidin-HRP reagent (Vectastain Elite ABC-HRP Kit ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were washed again with PBSt and incubated with an anti-human Fc HRP-conjugated antibody (ab977225, Abcam). Development was performed with TMB solution and terminated with Stop solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... 3 ml of the adjuvant goat IgG anti-human IgM Fc (5 ng/ml) (by ABCAM, Cambridge, UK) was dissolved in human albumin and phosphate-buffered normal saline added to the remainder of the sediments and packaged in 1 ml vials of injectable solution with labels (See Supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg of a murine isotype-matched antibody to control for Fc receptor binding (murine IgG2a/κ; Abcam), or phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Neuroscience 2020Quote: ... Netherlands).The His-tagged phage display antibody was detected by immunostaining with 6X-His Tag primary antibody (1:700, Abcam) and 1:500 Alexa-488 conjugated secondary antibody ...
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bioRxiv - Synthetic Biology 2021Quote: ... for Im7 variants or 50 μL of 1:5,000-diluted HRP-conjugated anti-6×His tag antibody (Abcam, catalog # ab1187) for scFv-HER2 variants ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed twice in PBST before being incubated with the primary rabbit anti-E-tag antibody diluted 1/5,000 in PBST (Abcam, ab3397) for 1h at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were incubated with goat polyclonal antibodies directed against MOMP and rabbit polyclonal antibodies directed against 6xHis tag (Abcam) for 1 hour ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysis contained GFP tag strain was subjected to affinity purification by using Anti–GFP antibody (ab290, Abcam, Cambridgeshire, UK). The process of antibody purification was using GenScript Protein A MagBeads according to the manufacturer’s instructions (Catalog No ...
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bioRxiv - Biochemistry 2021Quote: ... The bead slurries used were c-myc-conjugated (EZview red rabbit anti-c-myc affinity gel, Sigma, or Agarose goat anti-myc tag antibody, Abcam) and ubiquitin binding domain (UBD)-conjugated matrix (Ubiqapture-Q ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria harboring an HA tag were washed with PBS and stained with anti-HA antibody conjugated with FITC (Abcam ab1208) at 10 µg/mL for 75 minutes in the dark on ice ...
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bioRxiv - Cell Biology 2022Quote: ... stable expression of target receptors was determined by staining against their N-terminal Flag-tag with mouse anti-Flag conjugated to Alexa 488 (Abcam).
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were incubated with the blots overnight at 4 °C in blocking solution: anti-myc-tag (1:5,000, rabbit pAb, ab9106, Abcam, UK), anti-Dclk1 (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Induced EBY100 was concurrently labeled with DyLight-488-MMP-14 (see below) and 1:100 PE Anti-Myc tag antibody (abcam) for 1 h 30’ at room temperature in TNCA (50 mM Tris ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... The plates were washed four times with PBST and were then incubated with 1:15,000 rabbit polyclonal 6x His-tag antibody conjugated to HRP (Abcam, Austria) for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Molecular Biology 2024Quote: ... homozygous clones were further analysed by western blotting for homozygous knock-in of the tag using anti-UAP56 (ab181059; Abcam) and anti-GFP antibodies (A11122 ...
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bioRxiv - Cell Biology 2020Quote: ... Mt1-2 (Abcam ab12228 ...
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bioRxiv - Immunology 2019Quote: ... 2-deoxyglucose (Abcam), brefeldin A (BD) ...
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bioRxiv - Neuroscience 2020Quote: ... dynamin 2 (Abcam), synapsin ...
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bioRxiv - Neuroscience 2020Quote: ... β2 (Abcam, ab156000), BDNF (Abcam ...
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Metformin protects trabecular meshwork against oxidative injury via activating integrin/ROCK signalsbioRxiv - Pharmacology and Toxicology 2022Quote: ... ROCK1/2 (Abcam), MLC1 (Abcam) ...
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bioRxiv - Cancer Biology 2019Quote: ... or Fc-iNKG2D.AF and bound MicAbody detected with an HRP-conjugated mouse-anti-human kappa chain antibody (Abcam #ab79115). Affinity of both monomeric and antibody-fused ULBP2 variants was also determined by Octet analysis as described above.
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bioRxiv - Immunology 2021Quote: ... the antigens and a capture anti-human IgE (Mouse anti-Human IgE Fc) (ref. ab99834, Abcam PLC, Cambridge, UK) were coupled to magnetic MagPlex® 6.5μm COOH-microspheres (Luminex Corporation ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Immunology 2021Quote: ... before incubation with primary antibodies in blocking buffer (Rabbit polyclonal anti-SARS-Cov2, Sino Biological 40591-T62, 1:6000 dilution or Mouse b-actin, Abcam 8226, 1 μg/ml), detected with HRP conjugated secondaries in blocking buffer (Goat anti-Rabbit HRP ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...