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Citations for abcam :
551 - 600 of 1515 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... fibroblast activation protein (FAP, Abcam rabbit monoclonal antibody EPR20021 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by protein block (Abcam) for 30 minutes and were then incubated with relevant primary antibodies diluted in antibody diluent (Abcam ...
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bioRxiv - Immunology 2021Quote: ... or 10 µg/mL recombinant full length murine MMP9 without additional chemical activation by p-Aminophenylmercuric acetate (ab39309, Abcam). After 4 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The sequential sections from all collected tissues were incubated with a recombinant anti-Mouse IgG antibody [RM104] (ab190475, Abcam) performed as the negative control for the expression of viral antigen.
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bioRxiv - Immunology 2023Quote: Isolated human skin immune cells were treated with 10 µg/ml of recombinant HCMV-gB (Abcam, Cambridge, UK, ab43040) or 10 µg/ml of recombinant HCMV-gH (MyBioSource ...
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bioRxiv - Biophysics 2022Quote: ... The blocking buffer was removed and cells were incubated with recombinant anti-Lamin B1 primary antibody (ab229025, by abcam) diluted 1:250 (volume/volume ...
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bioRxiv - Cancer Biology 2023Quote: ... CAFs were pre-treated prior to co-cultures for 2h with recombinant label-free 100nM Annexin-V (#ab157342, Abcam) or 100 nM Zn-DPA (Molecular Targeting Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in either recombinant primary rabbit anti-GFAP antibody (astrocytes, 1:500 in 2% BSA and 0.1% Triton [TBS]; Abcam), or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Biochemistry 2019Quote: Lysosomal quantity was measured using the Lysosomal Staining Kit – Green Fluorescence – Cytopainter (Abcam). Cells were plated at 60,000 cells per well of a 96 well plate ...
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bioRxiv - Biochemistry 2019Quote: ATPase kit containing malachite green and ammonium molybdate was purchased from Abcam (ab65622). For assays in Figure 4b ...
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bioRxiv - Genetics 2019Quote: ... For mitochondria identification a stock of Mitochondrial Staining Reagent-Green (ab176830, Cytopainter, Abcam) was diluted 1:500 in H2O and 3 µl added to the colonies ...
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bioRxiv - Developmental Biology 2023Quote: ... were incubated with the cell tracking dye Cytopainter Green (ab138891, Abcam, 1:1000) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 μL hydrogel ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with the fluorescent labelling reagent Fluo-4 AM (at 1μM in D-MEM) for the detection of intracellular calcium (GR3332801, Abcam) for 1 hour at 35°C ...
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bioRxiv - Developmental Biology 2021Quote: ... De novo protein synthesis was measured using the Global Protein Synthesis Assay Kit (Abcam, ab235634) following the instruction provided by the vendor ...
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bioRxiv - Biochemistry 2021Quote: ... Carbonyls in protein samples were quantified using the Protein Carbonyl Content Assay Kit (Abcam, ab126287) according to the manual ...
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bioRxiv - Neuroscience 2020Quote: ... The molecular weights of the proteins were verified using a prestained protein ladder (ab116028, Abcam). Data were analyzed by densitometry in ImageJ using the gel quantification plugin.
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Guanine Nucleotide Binding protein (G protein Beta 3 subunit, GNB3; Abcam; 1:1000), rabbit anti-β-catenin (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were measured using the BIO-RAD Protein Assay or Bradford assay (Abcam 119216) and adjusted with apoplast extraction buffer to a final concentration between 0.5-1.5 mg/mL ...
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bioRxiv - Immunology 2023Quote: Protein A was quantified in the CM using the Protein A ELISA Kit (abcam, UK) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1 (HSC 70, Abcam Cat. # AB78431)
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bioRxiv - Molecular Biology 2022Quote: ... and then probed with primary antibody (mouse monoclonal anti-His from Pierce, 1:3000; rabbit monoclonal anti-β-Pol from Abcam, 1:2000) overnight ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315, Abcam, Cat.#ab6789, respectively). For HisTag purification ...
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bioRxiv - Immunology 2021Quote: ... and harvested into EDTA and used to measure plasma hemoglobin (plasma-Hb) and quantify soluble recombinant thrombomodulin (sTM-ELISA, ab209880, Abcam) and thrombin-antithrombin (TAT-ELISA ...
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bioRxiv - Cell Biology 2019Quote: ... was incubated with recombinant active PLK1 (0, 50 or 100 ng) or PLK1-kinase dead (100 ng) (purchased from Abcam or purified from sf9 insect cells as detailed above ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific; RRID:AB_1954821; Recombinant rabbit anti-phospho S129 alpha synuclein, 1:2000, Abcam 51253);the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and clone #124 (human + mouse selective antibody catalogue number ab256526) were chosen for recombinant antibody generation and are now commercially available from Abcam.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in a solution containing the Recombinant Anti-Iba1 antibody rabbit (ab178847, 1:500, abcam). After additional washes ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody produced in-house (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... recombinant IL-1-Ra at 40ng/mL (Biozol, #PPT-AF-2000-01RA ml and recombinant SLP-antibody at 5μg/mL (abcam, #ab191883), plasma diluted 1:20 of a patient with COVID-19 and high-titered IL-1-Ra-antibodies (Val 16 ...
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bioRxiv - Physiology 2022Quote: ... Samples were prepared using non-reducing sample buffer and were loaded (20 ug/well) onto the gelatin gel along with recombinant MMP9 (Abcam) and MMP2 (BioLegend ...
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bioRxiv - Plant Biology 2023Quote: ... CTR1-KD and MPK4 were analyzed by western blot using Recombinant Anti- Thiophosphate ester antibody (Abcam, Cat. Ab92570, 1:5000).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: recombinant rabbit anti-APOE mAb (1:300 IF; 1:50 IG; 1:500 WB; Abcam Cat# ab52607, RRID:AB_867704), mouse anti-HA-Tag mAb 6E2 (1:250 IF ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody recombinant anti-Fox2a antibody (ab108422) and secondary goat anti-rabbit IgG H&L HRP conjugated (ab6721) were obtained from abcam and used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Extracts corresponding to a known number of cells as well as recombinant 6xHis-PHB1 or 6xHis-PHB2 (Abcam, Cambridge, UK) were separated on 4-15% Mini-Protean® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: To treat cells with rPAI-1 we used recombinant active human PAI-1 (carrying stabilizing mutations N150K, K154T, Q319L and M354I, Abcam) in experiments on human airway epithelial cultures and in in-vitro complex formation assays ...
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bioRxiv - Microbiology 2022Quote: ... Some sections also were incubated with directly labeled fluorescent with Alexa Fluor® antibodies against CK5 (Abcam, cat. no. ab193894) or CK15 (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Goat pAb to MS IgG (Chromeo 546, ab60316), Dnk pAb to Rb IgG (Alexa Fluor 647, ab150075) fluorescent secondary antibodies were acquired from Abcam. Anti-GAPDH antibody ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Goat pAb to MS IgG (Chromeo 546, ab60316), Dnk pAb to Rb IgG (Alexa Fluor 647, ab150075) fluorescent secondary antibodies were obtained from Abcam. Nigericin ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Safranin O-fast green staining and immunohistochemical staining for COL2A1 (1:1000, Abcam, USA) were performed according to instruction manual ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was applied at a 1:1,000 dilution with 3 μM Nuclear Green DCS1 (Abcam) for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 (green)/Alexa Fluor 594 (red) conjugated secondary antibody (Abcam, Cambridge, MA) were then used to detect primary antibodies for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 (green)/Alexa Fluor 594 (red) conjugated secondary antibody (Abcam, Cambridge, MA) were then used to detect primary antibodies for 1h.
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bioRxiv - Neuroscience 2023Quote: ... GSH and GSSG levels were measured by a green fluorescence assay kit (Abcam, ab205811).
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bioRxiv - Developmental Biology 2024Quote: ... were incubated with the cell tracking dye Cytopainter Green (1:500, cat# ab138891, Abcam) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 uL hydrogel ...
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bioRxiv - Physiology 2021Quote: Carbonyl groups inserted into proteins by oxidative reactions were evaluated with protein carbonyl assay kit (Abcam). Briefly ...
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bioRxiv - Microbiology 2022Quote: The level of oxidized protein was measured with the Oxyblot kit (Protein Carbonyl Assay Kit, Abcam) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Target proteins were normalized to total cellular/housekeeping proteins: anti-α-actinin (Abcam, ab18061, 1:3000), anti-β-actin (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Protein-protein interactions between Galectin-3 and FcRn were studied using Co-Immunoprecipitation Kit (ab206996, Abcam) (see Supplementary methods).