Rat Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit citations for abcam

Quote: ... and anti-rat MOMA1 (Abcam #ab53443) primary antibodies overnight at 4°C followed by incubation with anti-rabbit Alexa 488 and anti-rat Alexa 555 secondary antibodies in 2% BSA ...

Extracellular RNA induce neutrophil recruitment via endothelial TLR3 during venous thrombosis after vascular injury

Maria Y. Najem, et al., bioRxiv - Physiology 2024

Quote: ... a rat anti-MOMA-2 (Abcam) and rabbit anti-mouse CitH3 (Abcam ...

Chromatin regulator Kdm6b is required for the establishment and maintenance of neural stem cells in mouse hippocampus

Eugene Gil, et al., bioRxiv - Neuroscience 2024

Quote: ... rat anti-BrdU (1:500, Abcam), mouse anti-NeuN (1:500 ...

BMP9 and BMP10 coordinate liver cellular crosstalk to maintain liver health

Dianyuan Zhao, et al., bioRxiv - Cell Biology 2024

Quote: ... donkey anti-rat AF488 (ab150153, Abcam), donkey anti-rat AF594 (A21209 ...

A neuron, microglia, and astrocyte triple coculture model to study Alzheimer disease

Celia Luchena, et al., bioRxiv - Neuroscience 2021

Quote: Levels of anti-inflammatory cytokine Transforming growth factor-β1 (TGF-β1) in supernatants were measured by an ELISA kit (ab119558, abcam, UK), following manufacturer’s instructions ...

The SARS-CoV-2 Spike protein disrupts human cardiac pericytes function through CD147-receptor-mediated signalling: a potential non-infective mechanism of COVID-19 microvascular disease

Elisa Avolio, et al., bioRxiv - Cell Biology 2021

Quote: The presence of S protein in COVID-19 patients’ serum was evaluated using the COVID-19 Spike Protein ELISA Kit from Abcam (ab274342), according to manufacturer’s instructions ...

Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus

Federico Cocozza, et al., bioRxiv - Microbiology 2020

Quote: Quantification of the amount of human ACE2 in the different EV and fractions was done using the human ACE2 ELISA kit (Abcam, ab235649) following manufacturer’s instructions.

Effects of Platycodon grandiflorum on Gut Microbiota and Immune System of Immunosuppressed Mouse

So Yun Jhang, et al., bioRxiv - Microbiology 2021

Quote: ... Serum concentrations of immunoglobulin A (IgA) and immuno-globulin M (IgM) were measured using enzyme-linked immunosorbent assays kits (ELISA; Abcam, USA), according to the manufacturer’s instructions ...

A facile method of mapping HIV-1 neutralizing epitopes using chemically masked cysteines and deep sequencing

Rohini Datta, et al., bioRxiv - Molecular Biology 2020

Quote: ... and virus replication was quantitated by measuring the p24 antigen released in culture supernatants at different time points using HIV-1 p24 SimpleStep ELISA kit® (Abcam). Serial dilutions of HIV-1 p24 protein (strain 92418 ...

Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance

Xiaopeng Tang, et al., bioRxiv - Immunology 2020

Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...

Tumor-educated monocyte-dendritic progenitors promote a metastatic switch

Ksenia Magidey-Klein, et al., bioRxiv - Cancer Biology 2020

Quote: ... IL-6 levels in TCM of met-low and met-high melanoma and breast carcinoma tumors were quantified using a specific enzyme-linked immunosorbent assay (ELISA) kit (ab46100, Abcam, US). The ELISA experiments were performed with 5-8 mice per group ...

A receptor-independent signaling pathway for BDNF

Julia Fath, et al., bioRxiv - Cell Biology 2022

Quote: ... Amphiregulin concentrations in HT-22 cell fractions were determined by using a Human Amphiregulin ELISA kit (ab222504; detection range: 18 – 600 pg/mL; Abcam, Netherlands). Protein concentrations were measured using RC DC Protein Assay (5000120 ...

An optimized Tet-On system for conditional control of gene expression in sea urchins

Jian Ming Khor, Charles A. Ettensohn, bioRxiv - Developmental Biology 2022

Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...

Hunting Drosophila viruses from wild populations: a novel isolation approach and characterization of viruses

Gaspar Bruner-Montero, et al., bioRxiv - Evolutionary Biology 2022

Quote: One hundred µl of the cell culture was transferred into a 96-well microplate provided by the In-Cell ELISA kit (Abcam, ab111541), and treated following the manufacturer’s recommendations ...

Chronic stress exposure alters the gut barrier: sex-specific effects on microbiota and jejunum tight junctions

Ellen Doney, et al., bioRxiv - Neuroscience 2022

Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...

Botulinum neurotoxin A modulates the axonal release of pathological tau in hippocampal neurons

Chiara Panzi, et al., bioRxiv - Neuroscience 2023

Quote: Conditioned media from the somatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer’s instructions ...

2-Thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses

Kentaro Uemura, et al., bioRxiv - Microbiology 2022

Quote: ... and nuclear DNA-encoded succinate dehydrogenase A (SDH-A) using the MitoBiogenesis In-Cell Enzyme-linked immunosorbent assay (ELISA) Kit (Colorimetric, Abcam, ab110217) following the manufacturer’s instructions ...

Conserved YKL-40 changes in mice and humans after postoperative delirium

Jennifer David-Bercholz, et al., bioRxiv - Neuroscience 2022

Quote: ELISA kits were used to quantitate the mouse plasma levels of YKL-40 (1:40 dilution, #AB238262; ABCAM, Cambridge, MA, USA), IL-6 (1:4-1:2 dilution ...

Regulation of VEGFR2 and AKT signaling by Musashi-2 in lung cancer

Igor Bychkov, et al., bioRxiv - Cell Biology 2023

Quote: The concentration of VEGF-A in human lung cancer cell lines was determined by a VEGF Human ELISA kit (Abcam, #ab100662). ELISA assays were performed according to the manufacturer manual ...

Gene Deletion of the PACAP/VIP Receptor, VPAC2R, Alters Glycemic Responses During Metabolic and Psychogenic Stress in Adult Female Mice

Elena V. Kozlova, et al., bioRxiv - Physiology 2023

Quote: The same plasma samples were diluted 10-fold and corticosterone (CORT) was measured using a commercial competitive ELISA kit (Abcam, AB108821). The assay had a sensitivity of 0.30 ng/ml in a standard range of 0.39 ng/ml to 100 ng/ml ...

PARP inhibitors enhance reovirus-mediated cell killing through the death-inducing signaling complex (DISC) with an associated NF-κB-regulated immune response

Joan Kyula-Currie, et al., bioRxiv - Cancer Biology 2023

Quote: ... The pellet was centrifuged once again and re-suspended in nuclear extraction buffer from the kit to generate the nuclear fraction and samples used for the NF-κB p65 transcription ELISA based assay (Abcam, ab133112) according to the manufacturer’s instructions.

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