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Citations for abcam :
201 - 250 of 289 citations for RNase Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies with respective dilutions were used for target detection: 1:10 000 anti-SFPQ (Abcam), 1:5000 anti-FUS (ProteinTech) ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was performed using mainly goat anti-rabbit IgG H&L (DyLight® 488) (1:500, Abcam ab96883), Cy3-donkey anti guinea pig IgG H+L (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Endogenous peroxidase and protein blocking steps were performed using reagents provided in the ABC detection kit (Abcam, ab64261). Sections were incubated overnight with primary antibodies at 4°C (Table S2) ...
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bioRxiv - Bioengineering 2024Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG as detection antibody were purchased from Abcam (Cambridge, UK). Capture antigens PDIA6 (ab101048) ...
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bioRxiv - Microbiology 2024Quote: Exponentially growing Leptospira (2×109) were used to determine ATP concentration using the luminescent ATP detection assay (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Levels of GSH and GSSG were measured using the GSH/GSSG Ratio Detection Assay Kit II (Abcam catalog # ab205811). NADPH and NADP+ were measured using the NADP/NADPH assay kit (Abcam catalog # 176724).
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bioRxiv - Immunology 2021Quote: ... The histological detection of ferric iron in the spleen was performed using an iron staining kit (ab 150674, Abcam).
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bioRxiv - Neuroscience 2020Quote: ... 7um sections of adipose tissues was performed for detection of UCP1 (RRID:AB_2241462, 1:500, Abcam, Cambridge, UK. Cat. #10983). Alexa 488 (2.5µg.mL ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with the fluorescent labelling reagent Fluo-4 AM (at 1μM in D-MEM) for the detection of intracellular calcium (GR3332801, Abcam) for 1 hour at 35°C ...
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection for BrdU was done with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam), together with mouse anti ssDNA (clone16-19 ...
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bioRxiv - Immunology 2020Quote: ... cells were activated as above for and then fixed and permeabilized followed by detection with pPyk2 Y402 (Abcam, ab4800) and secondary detection with goat anti-rabbit IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... respectively) were measured in GSC 8.11 and GSC 6.27 using a GSH/GSSG Ratio Detection Assay Kit (Abcam, ab138881) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in milk or BSA prior to detection with specific antibodies: 1:1000 ACE2 rabbit (Abcam, Ab108209), 1:5000 GAPDH rabbit (Abcam ...
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bioRxiv - Physiology 2022Quote: Skeletal muscle GSH and GSSG was measured using the fluorometric GSH/GSSG Ratio Detection Assay Kit II (Abcam 205811). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... 48h and 72h with 1 µM of (-)-ILV or 0.1% DMSO and apoptotic cells were analyzed by using Annexin V FITC apoptosis detection kit (Abcam). Following treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were lysed by 5% Total glutathione content was measured using an Intracellular glutathione (GSH) Detection Assay Kit (Abcam) as described in the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Metabolites were extracted and ATP concentration (mM/embryo) measured using a luminescent ATP Detection Assay kit (Abcam, catalog # ab113849), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... after which a Novolink Polymer detection system44 was used with an Abcam anti-NF-κB antibody (Abcam, Cambridge, UK) at a 1 in 1500 dilution ...
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bioRxiv - Immunology 2023Quote: A TUNEL assay to determine apoptosis was performed using an in situ Cell Death Detection kit (Abcam, Cambridge, UK). For this assay ...
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bioRxiv - Cell Biology 2023Quote: ... The efficiency of depletion was assayed by measuring intracellular ATP levels using Luminescent ATP Detection Assay Kit (Abcam ab113849) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Microbiology 2023Quote: ... The amount of histamine released into the culture supernatant was determined using a histamine detection kit (Abcam, Cambridge, MA) as described previously [19] ...
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bioRxiv - Cancer Biology 2023Quote: ... and specific band detection was achieved by the use of rabbit monoclonal anti-4E-BP1 (clone Y329, 1:2,000, ab32024, Abcam) and rabbit polyclonal anti-p-4E- BP1 antibody (1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... cAMP levels in the cells were determined in a plate reader by using fluorescence-based cAMP detection kit (Abcam) according to the manufacturer’s recommendations and normalized to the total protein present in the samples as determined by a BCA protein estimation kit (Pierce) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 1X Binding buffer with Annexin V and propidium iodide (PI) with a Annexin V-FITC Apoptosis Detection Kit (Abcam), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... adherent and floating cells were collected and labeled with the Annexin V-Cy3 Apoptosis Detection Kit (Abcam, Cambridge, UK, #ab14143) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Cells treated with 0.1% DMSO or 5 μM SGC-0946 were stained with Annexin V-FITC and DAPI following the protocol of the Annexin V-FITC Apoptosis Detection Kit (Abcam). Fluorescence was detected by FACS using the CyAn ADP Analyzer (Beckman Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: For the detection of BpOmpW-specific IgG in human plasma the same protocol was applied using anti-human IgG antibody (ab6858, abcam) in place of anti-mouse IgG antibodies.
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bioRxiv - Bioengineering 2021Quote: ... Spleen and brain tissues were formalin-fixed and paraffin-embedded blocks were used for immunofluorescence detection of GFP with anti GFP antibody (ab290 Abcam) or rabbit monoclonal anti-Iba1 antibody (ab178846 ...
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bioRxiv - Bioengineering 2021Quote: ... stained with 10 µl of AnnexinV-FITC and propidium iodide (PI) following manufacturer’s instructions (Annexin-V Apoptosis Staining Detection Kit, ab14084, Abcam, USA). Metabolic activity tests were performed using an alamarBlue cell viability assay (BUF012 ...
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bioRxiv - Plant Biology 2021Quote: Quantitative measurement of reactive oxygen species was carried out using DCFDA cellular ROS detection assay kit (Abcam, product number AB113851), and the assay was carried out following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... for 2 hours after which the cells were washed and stained with 1x Apoxin Green Indicator and 1x 7-AAD (Apoptosis/Necrosis detection kit, Abcam) for 45 min ...
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bioRxiv - Plant Biology 2019Quote: ... Protein detection was performed using a monoclonal rabbit anti-VEGF primary antibody (1:1000 dilution, ab52917, Abcam plc, Cambridge, UK) and a goat anti-rabbit HRP secondary antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissue sections were developed using the rabbit-specific HRP/DAB (ABC) Detection IHC Kit or Mouse on Mouse Polymer IHC Kit (Abcam). After haematoxylin counterstaining ...
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bioRxiv - Cell Biology 2019Quote: ... was used at 1:3500 dilution for imaging on the Li-Cor Odyssey detection system and goat anti-rabbit HRP (Abcam) was used for X-ray detection.
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bioRxiv - Immunology 2020Quote: ... The aerosol samples (suspended in 0.1% BSA) were examined for amylase activity (#ab102523, Abcam; detection limit of 0.2 umol/l) and found to contain <1 umol/l amylase suggesting minimal contamination by saliva ...
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bioRxiv - Bioengineering 2019Quote: ... The same protocol was applied for Strep-tag detection using a 1:1000 dilution of the anti-Strep-tag II antibody (ab76949, abcam) and a 1:10000 dilution of ‘Immun-Star Goat Anti-rabbit-HRP conjugate’ (170-5046 ...
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bioRxiv - Immunology 2021Quote: ... and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-ferret polyclonal IgG detection antibody (Abcam, Cambridge, UK) at a 1:4,000 dilution for a 90 min incubation at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... 7′-Dichlorofluorescin diacetate (DCFDA) was used to measure intracellular ROS using the DCFDA Cellular ROS Detection Assay Kit (ab113851, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... for detection (brown signals) of collagen type I (1:100 anti-human collagen I alpha-1 1200-1450 fragment (ab233080, Abcam) compatible to pig elastin [45] ...
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bioRxiv - Neuroscience 2022Quote: ... Streptavidin Peroxidase and DAB immunohistochemistry kits (Polyvalent HRP/DAB detection kit for Mouse and Rabbit Specific HRP/DAB, Abcam®) were used following manufacture instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The same protocol was used for β-actin detection using a rabbit polyclonal primary antibody (ab8227, Abcam, 1:1000 dilution). As noted by the manufacturer ...
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...
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bioRxiv - Cell Biology 2023Quote: ... pT320-pp1 (1:1000 for the detection of the endogenous protein, 1:30000 for the kinase assay, rabbit, Abcam ab62334), pY15-Cdk1 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated with the polymer rabbit detection system (DCS) and phH3 staining was detected after incubating the slides with DAB substrate kit (Abcam) for 10 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative detection of IgM/IgG in exosomes was performed according to the manufacturer’s instructions (IgM/IgG cow ELISA kit, Abcam, UK). The absorbance was determined on a plate reader using the contents of each well at 450 nm ...