Labshake search
Citations for abcam :
451 - 500 of 504 citations for Polybrominated Diphenyl Ether Surrogate Spiking Stock 10X Solution 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
-
bioRxiv - Neuroscience 2022Quote: ... retinas were incubated overnight at 4°C in the same solution containing a rabbit-anti-choline acetyltransferase (ChAT) monoclonal antibody (1:1000, Abcam ab178850, RRID:AB_2721842) and a guinea pig-anti-NeuN polyclonal antibody (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... For AMPA receptor-mediated miniature excitatory postsynaptic currents (mEPSCs) the aforementioned bath solution contained additionally 10 μM D-APV (Abcam, Cat# ab120003), 10 μM bicuculline methiodide (BMI ...
-
bioRxiv - Plant Biology 2023Quote: Immunoblotting was performed with antibodies diluted in the blocking solution (5% nonfat milk in TBS with 0.1% [v/v] Tween) at the following dilutions: α-GFP antibody (ab290, Abcam, Cambridge, UK), 1:8,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... and subsequently incubated for 1h at RT with the secondary antibody diluted in the blocking buffer solution: Donkey anti Rabbit 647 (1:1000, AB150075, ABCAM, Cambridge, UK); Donkey anti Mouse 594 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing chicken anti-GFP polyclonal antibody (1:1000; Abcam, Cambridge, MA) with gentle agitation at 4°C for 18–22 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... membranes were washed three times and incubated at 4°C overnight in 2% skim milk solution (1g skim milk powder, 50mL TBST) with the primary antibody (GFAP; #ab7260, Abcam, Cambridge, UK) and housekeeper protein (GAPDH ...
-
bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated ON at 4°C with the following primary antibodies in blocking solution: rabbit-anti-DYRK1A (Abcam, 1:100), mouse anti-DYRK1A (Abnova ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam, Cambridge, MA) for 90 minutes ...
-
bioRxiv - Physiology 2024Quote: ... Tissues were then incubated in a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) for two days at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
-
bioRxiv - Cancer Biology 2021Quote: ... with the following antibodies and conditions (dilution, heat-mediated antigen retrieval solution, retrieval time): anti-ERG (Abcam, clone EPR386, 1/100, H1, 30min); anti-p53 (Cell Signaling Technologies ...
-
bioRxiv - Cell Biology 2019Quote: ... the sections were incubated with blocking solution (Beyotime, P0102) for one hour at room temperature and incubated with primary antibody against BrdU (Abcam, ab6326, 1:100) overnight at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Tissues were blocked overnight at 4°C in 20v/v% goat serum in PBS and then incubated with primary antibody solution (CD31, Abcam-ab28364, 1:50) overnight followed by goat anti-Rabbit Alexa Fluor 488® (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... The membrane was then incubated overnight on a shaker at 4 °C in blocking solution amended with the primary antibody [anti-Lamin B1 (Abcam; ab16048; 1:1000), anti-GAPDH (Abcam ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were then transferred to primary antibody solution (anti-Chick GFP, Sigma, 1:1000 in PBS-T; rabbit anti-glutaminase (Abcam #ab156876, 1:1000); chicken anti-GFP (Abcam #ab13970 ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were blocked with 5% BSA and then incubated with the antibody solution (anti-pMLKL 1:200, Abcam; anti-Iba1 1:700, Wako), overnight at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min with primary antibodies diluted in blocking solution as follows: mouse anti-Pyruvate dehydrogenase E2/E3 (PDHE2/E3, ab110333, Abcam, 1 μgmL−1); mouse anti-clathrin heavy chain (X22 ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
-
bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113; 1:1000 Goat-α-rabbit AlexaFluor555, abcam ab150090) and washed again in PBS (4×10 min) ...
-
bioRxiv - Bioengineering 2020Quote: ... and PBS (twice, 5 min each) and left for 1 hour in block solution containing 10% goat serum (ab7481, Abcam, Cambridge, United Kingdom), 0.25% Triton-X 100 (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
-
bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in Inoue blocking solution (Inoue and Wittbrodt, 2011) (5 % Normal Goat Serum (NGS) (Abcam, cat# ab7481, lot# GR325285-5), 2% BSA (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... The sections were treated with PBS solution before being incubated with peroxidase-conjugated goat anti-rabbit IgG-HRP (Abcam, ab205718, dilution, 1:1000) for 20 minutes at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
-
bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... After washing with PBS a 2.5% BSA solution was applied for 1 hour and then incubated with primary anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903) and left over-night at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated at 4°C for 72 hours in blocking solution (5% bovine serum albumin, 0.2% Triton-X) containing primary antibodies CD45 antibody (Abcam, ab10558, 1:400 dilution), CD11b (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing goat anti-mouse IgM Alexa Fluor 488 (Abcam, ab150121, 1:500) antibody and goat anti-mouse IgG H&L Alexa Fluor 594 (Abcam ...
-
bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
-
bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
-
bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
-
bioRxiv - Bioengineering 2022Quote: ... The following primary antibodies were prepared in 1% BSA in PBS solutions at the following dilutions: rabbit anti-megalin (Abcam #ab76969, 1:500 dilution), mouse anti-aquaporin-1 (Santa Cruz #sc-32737 ...
-
bioRxiv - Neuroscience 2021Quote: ... for 1 h at room temperature and then with primary antibodies diluted in the blocking solution overnight (anti-PV primary antibody, ab11427, Abcam, Cambridge, UK; 1:1000) at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... The lower part of the membrane was blocked with 3% BSA in phosphate-buffered saline (PBS) solution followed by incubation with rabbit polyclonal anti-GFP (Abcam, UK; #ab290, 1:1,000) primary antibody at RT for 1 h for eGFP detection (30 kDa) ...
-
bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
-
bioRxiv - Neuroscience 2023Quote: ... and the washed pellet was resuspended in 250 ul of the following antibody solution: recombinant Alexa Fluor® 647 anti-ERG (1:100 dilution; ab196149, Abcam, Massachusetts, United States) in 0.5% BSA + 0.1% Triton in PBS ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
-
bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
-
bioRxiv - Neuroscience 2022Quote: ... the slices were incubated in the solution of primary antibodies: anti-glial fibrillar acidic protein (GFAP) rabbit polyclonal antibody (Abcam, Cambridge, UK, catalog number ab7260) and anti-neuronal nuclear protein (NeuN ...
-
bioRxiv - Plant Biology 2023Quote: ... and then was first incubated with rabbit anti-AKIN10 antibody (1:500 dilution) followed by incubation with Veriblot IP detection reagent (Abcam, 1:500 in blocking solution). Protein bands were detected with SupersignalTM west pico chemiluminescent substrate (Thermofisher ...